Osteoclast culture and resorption assays

Methods in Molecular Biology

Volumn 455, Issue , 2008, Pages 19-35

  Bradley, Elizabeth W ^a   Oursler, Merry Jo ^a  

^a MAYO CLINIC   (United States)

Author keywords

CD11b; CD14; Co culture; Osteoclast; RANKL; Resorption assay

Indexed keywords

EID: 84934437301     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-104-8_2     Document Type: Article

References (16)

1. Suda, T., et al. (1997) Regulation of osteoclast function. J Bone Miner Res 12, 869-879.
2. Udagawa, N., et al. (1990) Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells. Proc Natl Acad Sci U S A 87, 7260-7264.
3. Arai, F., et al. (1999) Commitment and differentiation of osteoclast precursor cells by the sequential expression of c-Fms and receptor activator of nuclear factor kappaB (RANK) receptors. J Exp Med 190, 1741-1754.
4. Wong, B. R., et al. (1997) TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells. J Biol Chem 272, 25190-25194.
5. Simonet, W. S., et al. (1997) Osteoprotegerin: a novel secreted protein involved in the regulation of bone density. Cell 89, 309-319.
6. Yasuda, H., et al. (1998) Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci U S A 95, 3597-3602.
7. Lacey, D. L., et al. (1998) Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 93, 165-176.
8. Udagawa, N., et al. (1989) The bone marrow-derived stromal cell lines MC3T3-G2/PA6 and ST2 support osteoclast-like cell differentiation in cocultures with mouse spleen cells. Endocrinology 125, 1805-1813.
9. Takahashi, N., et al. (1988) Osteoblastic cells are involved in osteoclast formation. Endocrinology 123, 2600-2602.
10. Raschke, W. C., et al. (1978) Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15, 261-267.
11. Sells Galvin, R. J., et al. (1999) TGF-beta enhances osteoclast differentiation in hematopoietic cell cultures stimulated with RANKL and M-CSF. Biochem Biophys Res Commun 265, 233-239.
12. Ragab, A. A., et al. (1998) Osteoclast differentiation requires ascorbic acid. J Bone Miner Res 13, 970-977.
13. Chambers, T. J., et al. (1984) Resorption of bone by isolated rabbit osteoclasts. J Cell Sci 66, 383-399.
14. Boyde, A., et al. (1986) Scanning electron microscopy in bone pathology: review of methods, potential and applications. Scan Electron Microsc Pt 4, 1537-1554.
15. Onyia, J. E., et al. (2004) Novel and selective small molecule stimulators of osteoprotegerin expression inhibit bone resorption. J Pharmacol Exp Ther 309, 369-379.
16. Fukayama, S., et al. (1988) Human parathyroid hormone (PTH)-related protein and human PTH: comparative biological activities on human bone cells and bone resorption. Endocrinology 123, 2841-2848.