Analysis of ssDNA gaps and DSBs in genetically unstable yeast cultures

Methods in Molecular Biology

Volumn 1170, Issue , 2014, Pages 501-515

  Peng, Jie ^a   Raghuraman, M K ^b   Feng, Wenyi ^a  

^a State University of New York Upstate Medical University: College of Medicine   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Chromosome fragile sites; DNA replication checkpoint; Double strand break; Hydroxyurea; Origins of replication; Replication stress; Single stranded DNA

Indexed keywords

CHECKPOINT KINASE 1; CHECKPOINT KINASE 2; HYDROXYUREA; PROTEIN KINASE; SINGLE STRANDED DNA;

ARTICLE; DNA DAMAGE; DNA REPLICATION; DOUBLE STRANDED DNA BREAK; FUNGAL CELL CULTURE; FUNGAL GENETICS; GENE MUTATION; GENOMIC INSTABILITY; LETHALITY; MICROARRAY ANALYSIS; MUTANT; NONHUMAN; PRIORITY JOURNAL;

EID: 84934436458     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-888-2_27     Document Type: Article

References (4)

1. Sogo JM, Lopes M, Foiani M (2002) Fork reversal and ssDNA accumulation at stalled replication forks owing to checkpoint defects. Science 297(5581):599-602
2. Feng W, Collingwood D, Boeck ME, Fox LA, Alvino GM, Fangman WL, Raghuraman MK, Brewer BJ (2006) Genomic mapping of singlestranded DNA in hydroxyurea-challenged yeasts identifies origins of replication. Nat Cell Biol 8(2):148-155
3. Feng W, Di Rienzi SC, Raghuraman MK, Brewer BJ (2011) Replication stress-induced chromosome breakage is correlated with replication fork progression and is preceded by single-stranded DNA formation. G3(Bethesda) 1(5):327-335
4. Di Rienzi SC, Lindstrom KC, Mann T, Noble WS, Raghuraman MK, Brewer BJ (2012) Maintaining replication origins in the face of genomic change. Genome Res 22(10):1940-1952