Methods for creating mutations in C. elegans that extend lifespan

Methods in Molecular Biology

Volumn 1048, Issue , 2013, Pages 65-75

  Wang, Dayong ^a   Cao, Min ^b   Dinh, Jessica ^b   Dong, Yuqing ^b  

^a MEDICAL SCHOOL OF SOUTHEAST UNIVERSITY   (China)

^b CLEMSON UNIVERSITY   (United States)

Author keywords

Caenorhabditis elegans; Forward genetics; Lifespan; Mutagenesis; Mutation; Outcross; Screen

Indexed keywords

ETHYLNITROSOUREA; MESYLIC ACID ETHYL ESTER; TRIMETHYLPSORALEN;

ARTICLE; CAENORHABDITIS ELEGANS; CELL SURVIVAL; CHEMICAL MUTAGENESIS; CONTROLLED STUDY; DNA DAMAGE; GENETIC SCREENING; HOMOZYGOSITY; INDUCED MUTATION; LIFE EXTENSION; MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROGENY;

ANIMALIA; CAENORHABDITIS ELEGANS;

AGING; ANIMALS; CAENORHABDITIS ELEGANS; GENES, HELMINTH; LIFE EXPECTANCY; LONGEVITY; MUTAGENESIS; MUTAGENS; MUTATION;

EID: 84934436273     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-556-9_6     Document Type: Article

References (25)

1. Luca D, d'Alessandro E, Bonacci S, Giraldi G (2011) Aging populations: The health and quality of life of the elderly. Clin Ter 162: E13-e18
2. Fontana L (2009) Modulating human aging and age-Associated diseases. Biochim Biophys Acta 1790:1133-1138
3. Pizza V, Agresta A, D'Acunto CW, Festa M, Capasso A (2011) Neuroinflamm-Aging and neurodegenerative diseases: An overview. CNS Neurol Disord Drug Targets 10:621-634
4. Helfand SL, Rogina B (2003) Genetics of aging in the fruit fly, Drosophila melanogaster. Annu Rev Genet 37:329-348
5. Kaeberlein M, Burtner CR, Kennedy BK (2007) Recent developments in yeast aging. PLoS Genet 3:e84
6. Kenyon CJ (2010) The genetics of ageing. Nature 464:504-512
7. Klass MR (1983) A method for the isolation of longevity mutants in the nematode Caenorhabditis elegans and initial results. Mech Ageing Dev 22:279-286
8. Grishok A (2005) RNAi mechanisms in Caenorhabditis elegans. FEBS Lett 579:5932-5939
9. Riddle DL, Blumenthal T, Meyer BJ et al (eds) (1997) Section II, induced mutations. C. elegans II, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
10. Flibotte S, Edgley ML, Chaudhry I, Taylor J, Neil SE, Rogula A, Zapf R, Hirst M, Butterfield Y, Jones SJ et al (2010) Whole-genome profiling of mutagenesis in Caenorhabditis elegans. Genetics 185:431-441
11. Anderson P (1995) Mutagenesis. Methods Cell Biol 48:31-58
12. De Stasio E, Lephoto C, Azuma L, Holst C, Stanislaus D, Uttam J (1997) Characterization of revertants of unc-93(e1500) in Caenorhabditis elegans induced by N-ethyl-N-nitrosourea. Genetics 147:597-608
13. De Stasio EA, Dorman S (2001) Optimization of ENU mutagenesis of Caenorhabditis elegans. Mutat Res 495:81-88
14. Jansen G, Hazendonk E, Thijssen KL, Plasterk RH (1997) Reverse genetics by chemical mutagenesis in Caenorhabditis elegans. Nat Genet 17:119-121
15. Barstead RJ, Moerman DG (2006) C. elegans deletion mutant screening. Methods Mol Biol 351:51-58
16. Byerly L, Cassada RC, Russell RL (1976) The life cycle of the nematode Caenorhabditis elegans. I. Wild-Type growth and reproduction. Dev Biol 51:23-33
17. Lesa GM (2006) Isolation of Caenorhabditis elegans gene knockouts by PCR screening of chemically mutagenized libraries. Nat Protoc 1:2231-2240
18. Hosono R (1978) Sterilization and growth inhibition of Caenorhabditis elegans by 5-fluorodeoxyuridine. Exp Gerontol 13:369-374
19. Mitchell DH, Stiles JW, Santelli J, Sanadi DR (1979) Synchronous growth and aging of Caenorhabditis elegans in the presence of fluorodeoxyuridine. J Gerontol 34:28-36
20. Gandhi S, Santelli J, Mitchell DH, Stiles JW, Sanadi DR (1980) A simple method for maintaining large, aging populations of Caenorhabditis elegans. Mech Ageing Dev 12:137-150
21. Hosono R, Mitsui Y, Sato Y, Aizawa S, Miwa J (1982) Life span of the wild and mutant nematode Caenorhabditis elegans. Effects of sex, sterilization, and temperature. Exp Gerontol 17:163-172
22. Aitlhadj L, Sturzenbaum SR (2010) The use of FUdR can cause prolonged longevity in mutant nematodes. Mech Ageing Dev 131:364-365
23. Dillin A, Hsu AL, Arantes-Oliveira N, Lehrer- Graiwer J, Hsin H, Fraser AG, Kamath RS, Ahringer J, Kenyon C (2002) Rates of behavior and aging specified by mitochondrial function during development. Science 298:2398-2401
24. Hansen M, Hsu AL, Dillin A, Kenyon C (2005) New genes tied to endocrine, metabolic, and dietary regulation of lifespan from a Caenorhabditis elegans genomic RNAi screen. PLoS Genet 1:119-128
25. Lambie EJ (2011) Mapping mutations in C. elegans. Methods Cell Biol 106:3-22