Purification and proteomic analysis of lysosomal integral membrane proteins

Methods in Molecular Biology

Volumn 432, Issue , 2008, Pages 229-241

  Zhang, Huiwen ^a   Fan, Xiaolian ^a   Bagshaw, Rick ^a,b   Mahuran, Don J ^a,b   Callahan, John W ^a,c  

^a HOSPITAL FOR SICK CHILDREN RESEARCH INSTITUTE   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

Lysosomes; Percoll; Proteomics; Tritosomes; Tyloxapol

Indexed keywords

RATTUS;

EID: 84934435338     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-28-7_16     Document Type: Article

References (17)

1. De Duve, C. (1973) The lysosome in retrospect. In Lysosomes in Biology and Pathology (Dingle, J. T. and Fell, H. B., eds.), pp. 3-40, North-Holland, Amsterdam.
2. Huynh, C., Roth, D., Ward, D. M., Kaplan, J. and Andrews, N. W. (2004) Defective lysosomal exocytosis and plasma membrane repair in Chediak-Higashi/beige cells. Proc. Natl. Acad. Sci. U.S.A. 101, 16795-16800.
3. Reddy, A., Caler, E. V. and Andrews, N. W. (2001) Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes. Cell 106, 157-169.
4. Huizing, M., Anikster, Y. and Gahl, W. A. (2001) Hermansky-Pudlak syndrome and Chediak-Higashi syndrome: disorders of vesicle formation and trafficking. Thromb. Haemost. 86, 233-245.
5. Perou, C. M., Leslie, C. M., Green, W., Li, L., McVey-Ward, D. and Kaplan, J. (1997) The Beige/Chediak-Higashi syndrome gene encodes a widely expressed cytosolic protein. J. Biol. Chem. 272, 29790-29794.
6. Introne, W., Boissy, R. E. and Gahl, W. A. (1999) Clinical, molecular, and cell biological aspects of Chediak-Higashi syndrome. Mol. Genet. Metab. 68, 283-303.
7. Bagshaw, R. D., Mahuran, D. J. and Callahan, J. W. (2005) A proteomics analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle. Mol. Cell. Proteomics 4, 133-143.
8. Yamada, H., Hayashi, H. and Natori, Y. (1984) A simple procedure for the isolation of highly purified lysosomes from normal rat liver. J. Biochem. 95, 1155-1160.
9. Nagle, D. L., Karim, M. A., Woolf, E. A., Holmgren, L., Bork, P., Misumi, D. J., et al. (1996) Identification and mutation analysis of the complete gene for Chediak-Higashi syndrome. Nat. Genet. 14, 307-311.
10. Karim, M. A., Suzuki, K., Fukai, K., Oh, J., Nagle, D. L., Moore, K. J., et al. (2002) Apparent genotype-phenotype correlation in childhood, adolescent, and adult Chediak-Higashi syndrome. Am. J. Med. Genet. 108, 16-22.
11. Cornillon, S., Dubois, A., Bruckert, F., Lefkir, Y., Marchetti, A., Benghezal, M., et al. (2002) Two members of the beige/CHS (BEACH) family are involved at different stages in the organization of the endocytic pathway in Dictyostelium. J. Cell Sci. 115, 737-744.
12. Warnock, D. E., Fahy, E. and Taylor, S. W. (2004) Identification of protein associations in organelles, using mass spectrometry-based proteomics. Mass Spectrom. Rev. 23, 259-280.
13. Brunet, S., Thibault, P., Gagnon, E., Kearney, P., Bergeron, J. J. M. and Desjardins, M. (2003) Organelle proteomics: looking at less to see more. Trends Cell Biol. 13, 629-838.
14. Bell, A. W., Ward, M. A., Blackstock, W. P., Freeman, H. N. M., Choudary, J. S., Lewis, A. P., et al. (2001) Proteomics characterization of abundant Golgi membrane proteins. J. Biol. Chem. 276, 5152-5165.
15. Leighton, F., Poole, B., Beaufay, H., Baudhuin, P., Coffey, J. W., Fowler, S., et al. (1968) The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions. J. Cell Biol. 37, 482-513.
16. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
17. Mortz, E., Krogh, T. N., Vorum, H. and Gorg, A. (2001) Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics 1, 1359-1363.