Confocal imaging of the microtubule cytoskeleton in C. elegans embryos and germ cells

Methods in Molecular Biology

Volumn 1075, Issue , 2014, Pages 257-272

  O'Connell, Kevin F ^a   Golden, Andy ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

C. elegans; Centrosomes; GFP; Immunofluorescence; Microtubules

Indexed keywords

FORMALDEHYDE; METHANOL;

ARTICLE; CAENORHABDITIS ELEGANS; CELL DIVISION; CELL POLARITY; CONFOCAL MICROSCOPY; CYTOSKELETON; EMBRYO; GERM CELL; GONAD; HERMAPHRODITISM; IMAGING; MICROTUBULE; NONHUMAN; OPTICAL RESOLUTION; PRIORITY JOURNAL; ROOM TEMPERATURE; SPERMATOGENESIS; STAINING; SURFACE PROPERTY;

CAENORHABDITIS ELEGANS;

ANIMALS; CAENORHABDITIS ELEGANS; CELL DIVISION; CELL POLARITY; CYTOSKELETON; GERM CELLS; MICROSCOPY, CONFOCAL; MICROTUBULES;

EID: 84934435199     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-847-8_13     Document Type: Article

References (16)

1. 2004 Centrosomes in Development and Disease. Edited by Erich A. Nigg copyright 2004 Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim ISBN 3-527-30980-2
2. Gard DL, Cha BJ, Schroeder MM (1995) Confocal immunofl uorescence microscopy of microtubules, microtubule-associated proteins, and microtubule-organizing centers during amphibian oogenesis and early development. Curr Top Dev Biol 31:383-431
3. Bowerman B (2001) Cytokinesis in the C. elegans embryo: regulating contractile forces and a late role for the central spindle. Cell Struct Funct 26:603-607
4. Goldstein B (2000) Embryonic polarity: a role for microtubules. Curr Biol 10:R820-R822
5. Gonczy P (2002) Mechanisms of spindle positioning: focus on fl ies and worms. Trends Cell Biol 12:332-339
6. Crittenden SL, Kimble J (1999) Confocal methods for Caenorhabditis elegans Methods Mol Biol 122:141-151
7. Miller DM, Shakes DC (1995) Immunofl uorescence microscopy. Methods Cell Biol 48:365-394
8. Aroian RV, Field C, Pruliere G, Kenyon C, Alberts BM (1997) Isolation of actin-associated proteins from Caenorhabditis elegans oocytes and their localization in the early embryo. EMBO J 16:1541-1549
9. Seydoux G, Dunn MA (1997) Transcriptionally repressed germ cells lack a subpopulation of phosphorylated RNA polymerase II in early embryos of Caenorhabditis elegans and Drosophila melanogaster Development 124: 2191-2201
10. Schedl T (1997) Developmental genetics of the germ line. In: Riddle DL, Blumenthal T, Meyer BJ, Priess JR (eds) C. elegans II. Cold Spring Harbor Laboratory Press, Plainview, NY, pp 241-269
11. L'Hernault SW (1997) Spermatogenesis. In: Riddle DL, Blumenthal T, Meyer BJ, Priess JR (eds) C. ELEGANS II. Cold Spring Harbor Laboratory Press, Plainview, NY, pp 271-294
12. Albertson DG, Thomson JN (1993) Segregation of holocentric chromosomes at meiosis in the nematode, Caenorhabditis elegans Chromosome Res 1:15-26
13. Verbrugghe KJ, White JG (2007) Cortical centralspindlin and G alpha have parallel roles in furrow initiation in early C. elegans embryos. J Cell Biol 120:1772-1778
14. O'Connell KF, Maxwell KN, White JG (2000) The spd-2 gene is required for polarization of the anteroposterior axis and formation of the sperm asters in the Caenorhabditis elegans zygote. Dev Biol 222:55-70
15. Golden A, Sadler PL, Wallenfang MR, Schumacher JM, Hamill DR, Bates G, Bowerman B, Seydoux G, Shakes DC (2000) Metaphase to anaphase (mat) transitiondefective mutants in Caenorhabditis elegans. J Cell Biol 151:1469-1482
16. L'Hernault SW, Roberts TM (1995) Cell biology of nematode sperm. Methods Cell Biol 48:273-301