Type a influenza virus detection and quantitation by real-time RT-PCR

Methods in Molecular Biology

Volumn 436, Issue , 2008, Pages 19-26

  Spackman, Erica ^a   Suarez, David L ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Avian influenza virus detection; Hydrolysis probe; Real time RT PCR; RNA; Virus quantitation

Indexed keywords

AVES; AVIAN INFLUENZA VIRUS; ORTHOMYXOVIRIDAE;

EID: 84934435117     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-279-3_4     Document Type: Article

References (8)

1. Spackman, E., Senne, D. A., Myers, T. J., Bulaga, L. L., Garber, L. P., Perdue, M. L., Lohman, K., Daum, L. T., and Suarez, D. L. (2002) Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J. Clin. Microbiol. 40, 3256-3260.
2. Spackman, E. and Suarez, D. L. (2005) Use of a novel virus inactivation method for a multi-center avian influenza real-time reverse transcriptase- polymerase chain reaction proficiency study. J. Vet. Diag. Invest. 17, 76-80.
3. Akey, B. L. (2003) Low-pathogenicity H7N2 avian influenza outbreak in Virgnia during 2002. Avian Dis. 47, 1099-1103.
4. Cattoli, G., Drago, A., Maniero, S., Toffan, A., Bertoli, E., Fassina, S., Terregino, C., Robbi, C., Vicenzoni, G., and Capua, I. (2004) Comparison of three rapid detection systems for type A influenza virus on tracheal swabs of experimentally and naturally infected birds. Avian Pathol. 33, 432-437.
5. Lee, C. W. and Suarez, D. L. (2004) Application of real-time RT-PCR for the quantitation and competitive replication study of H5 and H7 subtype avian influenza virus. J. Virol. Meth. 119, 151-158.
6. Holland, P. M., Abramson, R. D., Watson, R., and Gelfand, D. H. (1991) Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88, 7276-7280.
7. Wittwer, C. T., Herrmann, M. G., Moss, A. A., and Rasmussen, R. P. (1997) Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22, 130-131, 134-138.
8. Das, A., Spackman, E., Senne, D., Pedersen, J., and Suarez, D. L. (2006) Development of an internal positive control for rapid diagnosis of avian influenza virus infections by real-time reverse transcription-PCR with lyophilized eeagents. J. Clin. Microbiol. 44, 3065-3073.