Splint ligation of RNA with T4 DNA ligase

Methods in Molecular Biology

Volumn 941, Issue , 2012, Pages 257-269

  Kershaw, Christopher J ^a   O'Keefe, Raymond T ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

in vitro transcription; RNA ligation; RNA modification; RNA purification; T4 DNA ligase; T7 RNA polymerase

Indexed keywords

POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RNA LIGASE;

ARTICLE; CONTROLLED STUDY; DNA RNA HYBRIDIZATION; DNA TEMPLATE; HYBRIDIZATION; IN VITRO STUDY; PH; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN RNA BINDING; RNA DEGRADATION; RNA PURIFICATION;

EID: 84934434985     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-113-4_19     Document Type: Article

References (12)

1. Moore MJ, Sharp PA. Site-specific modification of pre-mRNA: the 2-hydroxyl groups at the splice sites. Science. 1992;256:992-7.
2. OKeefe RT, Newman AJ. Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO. 1998;17:565-74.
3. Juzumiene DI, Wollenzien P. Arrangement of the central pseudoknot region of 16S rRNA in the 30S ribosomal subunit determined by sitedirected 4-thiouridine crosslinking. RNA. 2001;7:71-84.
4. OKeefe RT, Norman C, Newman AJ. The invariant U5 snRNA loop 1 sequence is dispensable for thefirst catalytic step of pre-mRNA splicing in yeast. Cell. 1996;86:679-89.
5. Dix I, Russel CS, OKeefe RT, Newman AJ, Beggs JD. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA. 1998;4:1675-86.
6. Alvi RK, Lund M, OKeefe RT. ATP-dependent interaction of yeast U5 snRNA loop1 with the 5splice site. RNA. 2001;7:1013-23.
7. McGrail JC, Tatum E, OKeefe RT. Mutation in the U2 snRNA in fluences exon interactions of the U5 snRNA loop 1 during pre-mRNA splicing. EMBO. 2006;25:3813-22.
8. McGrail JC, OKeefe RT. The U1, U2 and U5 snRNAs crosslink to the 5exon during yeast pre-mRNA splicing. Nucleic Acids Res. 2008;36:814-25.
9. Tzakos AG, Easton LE, Lukavsky PJ. Perparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies. Nat Protoc. 2007;2:2139-47.
10. Kleppe K, van de Sande JH, Khorana HG. Polynucleotide ligase-catalyzed joining of deoxyribo-oligonucleotides on ribopolynucleotide templates and of ribo-oligonucleotides on deoxyribopolynucleotide templates. Proc Natl Acad Sci USA. 1970;67:68-73.
11. Fareed GC, Wilt EM, Richardson CC. Enzymatic breakage and joining of deoxyribonucleic acid. J Biol Chem. 1971;246: 925-32.
12. Sano H, Feix G. Ribonucleic acid ligase activity of deoxyribonucleic acid ligase from phage T4 infacted Escherichia coli . Biochemistry. 1974;13: 5110-5.