Hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism - Case study of Faecalibacterium prausnitzii

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Prévoteau, Antonin ^a   Geirnaert, Annelies ^a   Arends, Jan B A ^a   Lannebère, Sylvain ^b   Van De Wiele, Tom ^a   Rabaey, Korneel ^a  

^a GHENT UNIVERSITY   (Belgium)

^b UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOSE; RIBOFLAVIN;

ANAEROBIC GROWTH; BACTERIAL COUNT; ELECTROCHEMISTRY; ELECTRODE; GRAM POSITIVE BACTERIUM; HYDRODYNAMICS; KINETICS; METABOLISM; PROCEDURES; REPRODUCIBILITY; TIME FACTOR;

ANAEROBIOSIS; COLONY COUNT, MICROBIAL; ELECTROCHEMISTRY; ELECTRODES; GLUCOSE; GRAM-POSITIVE BACTERIA; HYDRODYNAMICS; KINETICS; REPRODUCIBILITY OF RESULTS; RIBOFLAVIN; TIME FACTORS;

EID: 84934324856     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11484     Document Type: Article

References (45)

1. Myers, C. R. & Nealson, K. H. Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. Science 240, 1319-1321 (1988).
2. Guerin, T., Mondido, M., McClenn, B. & Peasley, B. Application of resazurin for estimating abundance of contaminant-degrading micro-organisms. Lett. Appl. Microbiol. 32, 340-345 (2001).
3. Liu, C., Sun, T., Xu, X. & Dong, S. Direct toxicity assessment of toxic chemicals with electrochemical method. Anal. Chim. Acta 641, 59-63 (2009).
4. Catterall, K. et al. A sensitive, rapid ferricyanide-mediated toxicity bioassay developed using Escherichia coli. Talanta 82, 751-757 (2010).
5. Yong, D., Liu, C., Yu, D. & Dong, S. A sensitive, rapid and inexpensive way to assay pesticide toxicity based on electrochemical biosensor. Talanta 84, 7-12 (2011).
6. Ertl, P., Robello, E., Battaglini, F. & Mikkelsen, S. R. Rapid Antibiotic Susceptibility Testing via Electrochemical Measurement of Ferricyanide Reduction by Escherichia coli and Clostridium sporogenes. Anal. Chem. 72, 4957-4964 (2000).
7. Jordan, M. A., Welsh, D. T. & Teasdale, P. R. Ubiquity of activated sludge ferricyanide-mediated BOD methods: A comparison of sludge seeds across wastewater treatment plants. Talanta 125, 293-300 (2014).
8. Jordan, M. A., Welsh, D. T., John, R., Catterall, K. & Teasdale, P. R. A sensitive ferricyanide-mediated biochemical oxygen demand assay for analysis of wastewater treatment plant influents and treated effluents. Water Res. 47, 841-849 (2013).
9. Pasco, N., Baronian, K., Jeffries, C. & Hay, J. Biochemical mediator demand - a novel rapid alternative for measuring biochemical oxygen demand. Appl. Microbiol. Biotechnol. 53, 613-618 (2000).
10. Ikeda, T. Bioelectrochemical studies based on enzyme-electrocatalysis. Electrochim. Acta 82, 158-164 (2012).
11. Heiskanen, A. et al. Bioelectrochemical probing of intracellular redox processes in living yeast cells-application of redox polymer wiring in a microfluidic environment. Anal. Bioanal. Chem. 405, 3847-3858 (2013).
12. Rawson, F. J., Downard, A. J. & Baronian, K. H. Electrochemical detection of intracellular and cell membrane redox systems in Saccharomyces cerevisiae. Sci. Rep. 4 (2014).
13. Rabinowitz, J. D., Vacchino, J. F., Beeson, C. & McConnell, H. M. Potentiometric Measurement of Intracellular Redox Activity. J. Am. Chem. Soc. 120, 2464-2473 (1998).
14. Thorne, R. J., Hu, H., Schneider, K. & Cameron, P. J. Trapping of redox-mediators at the surface of Chlorella vulgaris leads to error in measurements of cell reducing power. Phys. Chem. Chem. Phys. 16, 5810-5816 (2014).
15. Nagamine, K., Kaya, T., Yasukawa, T., Shiku, H. & Matsue, T. Application of microbial chip for amperometric detection of metabolic alteration in bacteria. Sens. Actuator B-Chem. 108, 676-682 (2005).
16. Parce, J. W. et al. Detection of cell-affecting agents with a silicon biosensor. Science 246, 243-247 (1989).
17. Shiku, H., Nagamine, K., Kaya, T., Yasukawa, T. & Matsue, T. Bioelectrochemistry 249-266 (John Wiley & Sons, Ltd, 2008).
18. Richardson, N. J., Gardner, S. & Rawson, D. M. A chemically mediated amperometric biosensor for monitoring eubacterial respiration. J. Appl. Bacteriol. 70, 422-426 (1991).
19. Yip, N.-C., Rawson, F. J., Tsang, C. W. & Mendes, P. M. Real-time electrocatalytic sensing of cellular respiration. Biosens. Bioelectron. 57, 303-309 (2014).
20. Heiskanen, A. et al. Mediator-assisted simultaneous probing of cytosolic and mitochondrial redox activity in living cells. Anal. Biochem. 384, 11-19 (2009).
21. Catterall, K. et al. The use of microorganisms with broad range substrate utilisation for the ferricyanide-mediated rapid determination of biochemical oxygen demand. Talanta 55, 1187-1194 (2001).
22. Ikeda, T., Kurosaki, T., Takayama, K., Kano, K. & Miki, K. Measurements of Oxidoreductase-like Activity of Intact Bacterial Cells by an Amperometric Method Using a Membrane-Coated Electrode. Anal. Chem. 68 (1996).
23. Bard, A. J. & Faulkner, L. R. Electrochemical Methods: Fundamentals and Applications (2nd ed.) (New York, Wiley, 2001).
24. Ertl, P., Unterladstaetter, B., Bayer, K. & Mikkelsen, S. R. Ferricyanide reduction by Escherichia coli: kinetics, mechanism, and application to the optimization of recombinant fermentations. Anal. Chem. 72, 4949-4956 (2000).
25. Torimura, M., Miki, A., Wadano, A., Kano, K. & Ikeda, T. Electrochemical investigation of cyanobacteria Synechococcus sp. PCC7942-catalyzed photoreduction of exogenous quinones and photoelectrochemical oxidation of water. J. Electroanal. Chem. 496, 21-28 (2001).
26. Kasuno, M. et al. Characterization of the photoinduced electron transfer reaction from the photosynthetic system in Rhodobacter sphaeroides to an exogenous electron acceptor. J. Electroanal. Chem. 636, 101-106 (2009).
27. Duncan, S. H., Hold, G. L., Harmsen, H. J., Stewart, C. S. & Flint, H. J. Growth requirements and fermentation products of Fusobacterium prausnitzii, and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 52, 2141-2146 (2002).
28. Heinken, A. et al. Functional Metabolic Map of Faecalibacterium prausnitzii, a Beneficial Human Gut Microbe. J. Bacteriol. 196, 3289-3302 (2014).
29. Sokol, H. et al. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proc. Natl. Acad. Sci. U.S.A. 105, 16731-16736 (2008).
30. Khan, M. T. et al. The gut anaerobe Faecalibacterium prausnitzii uses an extracellular electron shuttle to grow at oxic-anoxic interphases. ISME Journal 6, 1578-1585 (2012).
31. Gutierrez, C. N. & Blevins, R. D. The Cannabinoids: Chemical, Pharmacologic, and Therapeutic Aspects 327-341 (Academic Press, 1984).
32. Bruggeman, Y. E. et al. Monoclonal antibodies against two electron reduced riboflavin and a quantification of affinity constants for this oxygen-sensitive molecule. Eur. J. Biochem. 234, 245-250 (1995).
33. Miquel, S. et al. Ecology and metabolism of the beneficial intestinal commensal bacterium Faecalibacterium prausnitzii. Gut Microbes 5, 146-151 (2014).
34. Ikeda, T. & Kano, K. Bioelectrocatalysis-based application of quinoproteins and quinoprotein-containing bacterial cells in biosensors and biofuel cells. Biochim. Biophys. Acta 1647, 121-126 (2003).
35. Ikeda, T. & Kano, K. An electrochemical approach to the studies of biological redox reactions and their applications to biosensors, bioreactors, and biofuel cells. J. Biosci. Bioeng. 92, 9-18 (2001).
36. Tatsumi, H., Kano, K. & Ikeda, T. Kinetic Analysis of Fast Hydrogenase Reaction of Desulfovibrio vulgaris Cells in the Presence of Exogenous Electron Acceptors. J. Phys. Chem. B. 104, 12079-12083 (2000).
37. Tatsumi, H., Takagi, K., Fujita, M., Kano, K. & Ikeda, T. Electrochemical Study of Reversible Hydrogenase Reaction of Desulfovibrio vulgaris Cells with Methyl Viologen as an Electron Carrier. Anal. Chem. 71, 1753-1759 (1999).
38. Perez, F. G., Mascini, M., Tothill, I. E. & Turner, A. P. F. Immunomagnetic Separation with Mediated Flow Injection Analysis Amperometric Detection of Viable Escherichia coli O157. Anal. Chem. 70, 2380-2386 (1998).
39. Kondo, T. & Ikeda, T. Rapid detection of substrate-oxidizing activity of hiochi bacteria using benzoquinone-mediated amperometric method. J. Biosci. Bioeng. 90, 217-219 (2000).
40. Lindqvist, R. Estimation of Staphylococcus aureus Growth Parameters from Turbidity Data: Characterization of Strain Variation and Comparison of Methods. Appl. Environ. Microbiol. 72, 4862-4870 (2006).
41. Mayhew, S. G. Studies on flavin binding in flavodoxins. Biochim. Biophys. Acta 235, 289-302 (1971).
42. Rosenbaum, M. A. et al. Shewanella oneidensis in a lactate-fed pure-culture and a glucose-fed co-culture with Lactococcus lactis with an electrode as electron acceptor. Bioresour. Technol. 102, 2623-2628 (2011).
43. Geirnaert, A. et al. Butyricicoccus pullicaecorum, a butyrate producer with probiotic potential, is intrinsically tolerant to stomach and small intestine conditions. Anaerobe 30, 70-74 (2014).
44. Miyazaki, K., Martin, J. C., Marinsek-Logar, R. & Flint, H. J. Degradation and utilization of xylans by the rumen anaerobe Prevotella bryantii (formerly P. ruminicola subsp. brevis) B14. Anaerobe 3, 373-381 (1997).
45. Van Nevel, S., Koetzsch, S., Weilenmann, H. U., Boon, N. & Hammes, F. Routine bacterial analysis with automated flow cytometry. J. Microbiol. Methods 94, 73-76 (2013).