Autotetraploid plant regeneration by indirect somatic embryogenesis from leaf mesophyll protoplasts of diploid Gentiana decumbens L.f.

In Vitro Cellular and Developmental Biology - Plant

Volumn 51, Issue 3, 2015, Pages 350-359

  Tomiczak, Karolina ^a   Mikuła, Anna ^a   Sliwinska, Elwira ^b   Rybczyński, Jan J ^a  

^a POLISH ACADEMY OF SCIENCES   (Poland)

^b UNIVERSITY OF TECHNOLOGY AND LIFE SCIENCES   (Poland)

Author keywords

Chromosome number; Flow cytometry; Gentian; Protoplast culture; Somaclonal variation

Indexed keywords

GENTIANA; GENTIANA DECUMBENS;

EID: 84931562995     PISSN: 10545476     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11627-015-9674-0     Document Type: Article

References (48)

1. Bergounioux C, Perennes C, Brown SC, Sarda C, Gadal P (1988) Relation between protoplast division, cell-cycle stage and nuclear chromatin structure. Protoplasma 142:127–136
2. Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice, a revised edition. Studies in plant science 5. Elsevier Science BV, Amsterdam
3. Cai Y, Liu Y, Liu Z, Zhang F, Fe X, Xia G (2009) High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim. In Vitro Cell Dev Biol Plant 45:730–739
4. Chen Z, Hsiao K-C, Bornman CH (1994) Ploidy and division efficiency of petiolar protoplasts of Brassica napus. Hereditas 120:41–46
5. Davey MR, Anthony P, Power JB, Lowe KC (2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv 23:131–171
6. Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87:81–89
7. Dhooghe E, Van Laere K, Eeckhaut T, Leus L, Van Huylenbroeck J (2011) Mitotic chromosome doubling of plant tissues in vitro. Plant Cell Tissue Organ Cult 104:359–373
8. Dungerdorj D, Tsetsegmaa S, Purevsuren S, Bayasgalan B (2006) On the phytochemical investigation of the genus Gentiana used for the treatment of liver diseases in Mongolian Traditional Medicine. Postępy Fitoterapii 2:71–74
9. Elmaghrabi A, Ochatt S (2006) Isoenzymes and flow cytometry for the assessment of true-to-typeness of calluses and cell suspensions of barrel medic prior to regeneration. Plant Cell Tissue Organ Cult 85:31–43
10. Fiuk A, Rybczyński JJ (2007) The effect of several factors on somatic embryogenesis and plant regeneration in protoplast cultures of Gentiana kurroo (Royle). Plant Cell Tissue Organ Cult 91:263–271
11. Fiuk A, Rybczyński JJ (2008a) Factors influencing efficiency of somatic embryogenesis of Gentiana kurroo (Royle) cell suspension. Plant Biotechnol Rep 2:33–39
12. Fiuk A, Rybczyński JJ (2008b) Genotype and plant growth regulator-dependent response of somatic embryogenesis from Gentiana spp. leaf explants. In Vitro Cell Dev Biol Plant 44:90–99
13. Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33:130–137
14. Ho TN, Liu SW (1990) The infrageneric classification of Gentiana (Gentianaceae). Bull Br Mus Nat Hist (Bot) 20:169–192
15. Ho TN, Pringle JS (1995) Gentianaceae. Flora China 16:1–139
16. Hosokawa K, Nakano M, Oikawa Y, Yamamura S (1996) Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana. Plant Cell Rep 15:578–581
17. Jomori H, Takahata Y, Kaizuma N (1995) Plant regeneration from leaf-derived calli of gentians and their protoplast culture. Acta Horticult 392:81–86
18. Karp A (1994) Origins, causes and uses of variation in plant tissue cultures. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Kluwer Academic Publishers, Dordrecht, pp 139–152
19. Kletter C, Glasl S, Thalhammer T, Narantuya S (2008) Traditional Mongolian Medicine—a potential for drug discovery. Sci Pharm 76:49–63
20. Köhlein F (1991) Gentians. Timber Press, Portland
21. Konieczny R, Sliwinska E, Pilarska M, Tuleja M (2012) Morphohistological and flow cytometric analyses of somatic embryogenesis in Trifolium nigrescens Viv. Plant Cell Tissue Organ Cult 109:131–141
22. Kunitake H, Nakashima T, Mori K, Tanaka M, Mii M (1995) Plant regeneration from mesophyll protoplasts of lisianthus (Eustoma grandiflorum) by adding activated charcoal into protoplast culture medium. Plant Cell Tissue Organ Cult 43:59–65
23. Larkin PJ (1976) Purification and viability determinations of plant protoplasts. Planta 128:213–216
24. Lindsay GC, Hopping ME, O’Brien IEW (1994) Detection of protoplast-derived DNA tetraploid Lisianthus (Eustoma grandiflorum) by leaf and flower characteristics and by flow cytometry. Plant Cell Tissue Organ Cult 38:53–55
25. Lukaszewska E, Virden R, Sliwinska E (2012) Hormonal control of endoreduplication in sugar beet (Beta vulgaris L.) seedlings growing in vitro. Plant Biol 14:216–222
26. Magnien E, Dalschaert X, Faraoni-Sciamanna P (1982) Transmission of a cytological heterogeneity from the leaf to the protoplasts in culture. Plant Sci Lett 25:291–303
27. Meng Y, Gao Y, Jia J (1996) Plant regeneration from protoplasts isolated from callus of Gentiana crassicaulis. Plant Cell Rep 16:88–91
28. Mikuła A, Rybczyński JJ (2001) Somatic embryogenesis of Gentiana genus I. The effect of the preculture treatment and primary explant origin on somatic embryogenesis of Gentiana cruciata (L.), G. pannonica (Scop.), and G. tibetica (King). Acta Physiol Plant 23:15–25
29. Mikuła A, Skierski J, Rybczyński JJ (2002) Somatic embryogenesis of Gentiana genus III. Characterization of three-year-old embryogenic suspensions of G. pannonica originated from various seedling explants. Acta Physiol Plant 24:311–322
30. Mishiba K, Yamane K, Nakatsuka T, Nakano Y, Yamamura S, Abe J, Kawamura H, Takahata Y, Nishihara M (2009) Genetic relationships in the genus Gentiana based on chloroplast DNA sequence data and nuclear DNA content. Breed Sci 59:119–127
31. Momčilović I, Grubišić D, Nešković M (1997) Micropropagation of four Gentiana species (G. lutea, G. cruciata, G. purpurea and G. acaulis). Plant Cell Tissue Organ Cult 49:141–144
32. Morgan ER, Hofmann BL, Grant JE (2003) Production of tetraploid Gentiana triflora var. japonica ‘Royal Blue’ plants. N Z J Crop Hortic Sci 31:65–68
33. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
34. Myagmar BE, Aniya Y (2000) Free radical scavenging action of medicinal herbs from Mongolia. Phytomedicine 7:221–229
35. Nakano M, Hosokawa K, Oomiya T, Yamamura S (1995) Plant regeneration from protoplasts of Gentiana by embedding protoplasts in gellan gum. Plant Cell Tissue Organ Cult 41:221–227
36. Nassour M, Chassériaux G, Dorion N (2003) Optimization of protoplast-to-plant system for Pelargonium × hortorum ‘Alain’ and genetic stability of the regenerated plants. Plant Sci 165:121–128
37. Nishihara M, Nakatsuka T, Hosokawa K, Yokoi T, Abe Y, Mishiba K, Yamamura S (2003) Dominant inheritance of white-flowered and herbicide-resistant traits in transgenic gentian plants. Plant Biotechnol 23:25–31
38. O’Brien IEW, Lindsay GC (1993) Protoplasts to plants of Gentianaceae. Regeneration of lisianthus (Eustoma grandiflorum) is affected by calcium ion preconditioning, osmolarity and pH of the culture media. Plant Cell Tissue Organ Cult 33:31–37
39. Ochatt SJ, Mousset-Déclas C, Rancillac M (2000) Fertile pea plants regenerate from protoplasts when calluses have not undergone endoreduplication. Plant Sci 156:177–183
40. Qureshi RA, Gilani SA, Ashraf M (2007) Ethnobotanical studies with special reference to plant phenology at Sudhan Gali and Ganga Chotti Hills (District Bagh, A.K.). EJEAFChe 6:2207–2215
41. Rybczyński JJ, Davey M, Tomiczak K, Fiuk A, Mikuła A (2015) Systems of plant regeneration in gentian in vitro cultures. In: Rybczyński JJ, Davey M, Mikuła A (eds). The Gentianaceae. Biotechnology and application vol. 2. Springer (in press)
42. Sari N, Abak K, Pitrat M (1999) Comparison of ploidy level screening methods in watermelon: Citrullus lanatus (Thunb.) Matsum. and Nakai. Sci Hortic 82:265–277
43. Sheng X, Zhao Z, Yu H, Wang J, Xiaohui Z, Gu H (2011) Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis). Plant Cell Tissue Organ Cult 107:513–520
44. Sliwinska E, Zielinska E, Jedrzejczyk I (2005) Are seeds suitable for flow cytometric estimation of plant genome? Cytometry 64A:72–79
45. Takagi H, Sugawara S, Saito T, Tasaki H, Yuanxue L, Kaiyun G, Han D-S, Godo T, Nakano M (2011) Plant regeneration via direct and indirect adventitious shoot formation and chromosome doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder. Plant Biotechnol Rep 5:187–195
46. Takahata Y, Jomori H (1989) Plant regeneration from mesophyll protoplasts of Gentiana (Gentiana scabra Bungei). Plant Tissue Cult Lett 6:19–21
47. Takahata Y, Jomori H, Miyano S, Kunitake H, Mii M (1995) Regeneration of plants from protoplasts of Gentiana species (Gentian). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol. 34. Plant protoplasts and genetic engineering VI. Springer, Berlin, pp 57–62
48. Thiem B, Sliwinska E (2003) Flow cytometry analysis of nuclear DNA content in cloudberry (Rubus chamaemorus L.) in vitro cultures. Plant Sci 164:129–134