Optimization of protoplast-to-plant system for Pelargonium x hortorum 'Alain' and genetic stability of the regenerated plants

Plant Science

Volumn 165, Issue 1, 2003, Pages 121-128

  Nassour, M ^a   Chasseriaux G ^a   Dorion, N ^a  

^a Institut National d'Horticulture   (France)

Author keywords

Mesophyll protoplasts; Mother plant culture; Nitrogen and calcium effects; Regeneration

Indexed keywords

CELLS; DNA; ENZYMES;

PLANT REGENERATION;

PLANTS (BOTANY);

PELARGONIUM; PELARGONIUM HORTORUM; PELARGONIUM X HORTORUM;

EID: 0038082040     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(03)00150-X     Document Type: Article

References (38)

1. K.B. Dunbar, C.T. Stephens, Plant regeneration from callus-derived protoplasts of Pelargonium x domesticum, Plant Cell Rep. 10 (1991) 417-420.
2. S.T. Nameth, M.L. Daughtry, G.W. Moorman, M.A. Sulzinski, Bacterial blight of geranium: a history of diagnostic challenges, Plant Dis. 83 (1999) 204-211.
3. J.F. Knauss, J. Tammen, Resistance of Pelargonium to Xanthomonas pelargonii, Pytopathology 57 (1967) 1178-1181.
4. L.C. Ewart, Utilization of flower germplasm, HortScience 16 (1981) 135-138.
5. C. Bigot, Multiplication végétative in vitro à partir de plantules d'hybrides F1 dc Pelargonium x hortorum Bailey, Act Horticulturae 131 (1983) 61-70.
6. B.N.S. Murthy, R.P. Singh, P.K. Saxena, Induction of high-frequency somatic embryogenesis in geranium (Pelargonium x hortorum Bailey cv Ringo Rose) cotyledonary cultures, Plant Cell Rep. 15 (1996) 423-426.
7. T. Kameya, Culture of protoplasts from chimeral plant tissue of nature, Jpn. J. Genet. 50 (1975) 417-420.
8. S.A. Yarrow, E.C. Coking, J.B. Power, Plant regeneration from culture cell-derived protoplasts of Pelargonium aridum, P x hortorum and P x pellatum, Plant Cell Rep. 6 (1987) 102-104.
9. M. Nassour, N. Dorion, Plant regeneration from protoplasts of micropropagated Pelargonium x hortorum 'Alain': effect of some environmental and medium factors on protoplast system efficiency, Plant Sci. 163 (2002) 169-176.
10. M.A. Revilla, S.J. Ochatt, S. Doughty, J.B. Power, A general strategy for the isolation mesophyll protoplasts from deciduous fruit and nut tree species, Plant Sci. 50 (1987) 133-137.
11. M. Nakano, M. Mii, Protoplast culture and plant regeneration of several species in the genus Dianthus, Plant Cell Rep. 11 (1992) 225-228.
12. M.M. Chang, W.H. Loescher, Effect of preconditioning and isolation conditions on potato (Solarium tuberosum L. cv. Russet Burbank) protoplast yield for shoot regeneration and electroporation, Plant Sci. 73 (1991) 103-109.
13. R.V. Sairam, N. Seetharama, P.S. Devi, A. Verma, U.R. Murthy, I. Potrykus, Culture and regeneration of mesophyll-derived protoplasts of sorghum (Sorghum bicolor L. Moench), Plant Cell Rep. 18 (1999) 972-977.
14. G. Patnaik, D. Wilson, E.C. Cocking, Importance of enzyme purification for increased plating efficiency and plant regeneration from single protoplasts of Petunia parodii, Z. Pflanzenphysiol. Bd. 102 (1981) 199-205.
15. J. Prakash, M.J. Foxe, Use of cellulase 'onozuka' RS and pectolyase Y-23 in isolation leaf mesophyll protoplasts from two recalcitrant potato cultivars, Ir. J. Agric. Res. 23 (1984) 99-103.
16. H. David, E. Jarlet, A. David, Effects of nitrogen source, calcium concentration and osmotic stress on protoplasts and protoplast-derived cell cultures of Pinus pinaster cotyledons, Physiol. Plant 61 (1984) 477-482.
17. J.P. Bourgin, C. Missonier, Culture of haploid mesophyll protoplasts from Nicotiana alata, Z. Pflanzenphysiol. Bd. 87 (1978) 55-64.
18. C. Bellini, M.C. Chupeau, M. Gervais, G. Vastra, Y. chupeau, Importance of myo-inositol, calcium, and ammonium for the viability and division of tomato (Lycopersicon esculentum) protoplasts, Plant Cell Tissue Org. Cult. 23 (1990) 27-37.
19. A. Grapin, C. Huppel, C. Marche, N. Dorion, First results in Pelargonium shoot apices cryopreservation, Acta Hortic. 560 (2002) 157-160.
20. T. Murashige, F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant 15 (1962) 473-497.
21. G. Morel, R.H. Wetmore, Fern callus tissue culture, Ann. J. Bot. 38 (1951) 141-143.
22. G.M.L. Kroes, R.P. Baayen, W. Lange, Histology of root rot of flax seedings (Linum usitatissimum) infected by Fusarium oxysporum f. sp. lini, Eur. J. Plant Pathol. 104 (1998) 725-736.
23. E.M. Frearson, J.B. Power, E.C. Cocking, The isolation, culture and regeneration of Petunia leaf protoplasts, Dev. Biol. 33 (1973) 130-137.
24. D. Marie, S.C. Brown, A cytometric exercise in plant DNA histograms, with 2C values for 70 species, Biol. Cell. 78 (1993) 41-51.
25. Q. Yang, S. Laliberté, Cell division and microcolony formation in protoplasts from mature material of hybrid larch (Larix x eurolepis Henry), Plant Sci. 117 (1996) 159-165.
26. M.A.L. Smith, J.P. Palta, B.H. McCown, Comparative anatomy and physiology of microcultured, seedling, and greenhouse-grown Asian white bireh, J. Am. Soc. Hort. Sci. 111 (1986) 437-442.
27. D. Yue, A. Gosselin, Y. Desjardins, Effects of forced ventilation at different relative humidities on growth, photosynthesis and transpiration of geranium plantlets in vitro, Can. J. Plant Sci. 73 (1993) 249-256.
28. A.W. Metwally, B.E. Struckmeyer, G.E. Beck, Effcct of three soil moisture regimes on the growth and anatomy of Pelargonium hortorum, J. Am. Soc. Hort. Sci. 95 (1970) 803-808.
29. K.K. Kartha, M.R. Michayluk, K.N. Kao, O.L. Gamborg, F. Constabel, Callus formation and plant regeneration from mesophyll protoplasts of rape plants (Brassica napus L. cv. Zephyr), Plant Sci. 3 (1974) 265-271.
30. F. Jullien, F. Diemer, M. Colson, O. Faure, An optimising protocol for protoplast regeneration of three peppermint cultivars (Mentha x piperita), Plant Cell Tissue Org. Cult. 54 (1998) 153-159.
31. N. Dorion, N. Wies, A. Burteaux, C. Bigot, Protoplast and leaf explant culture of Lycopersicom cheesmanii and salt tolerance of protoplast-derived calli, Plant Cell Tissue Org. Cult. 56 (1999) 9-16.
32. E.F. Berrios, L. Gentzbittel, H. Serieys, G. Alibert, A. Sarrafi, Influence of genotype and gelling agents on in vitro regeneration by organogenesis in sunflower, Plant Cell Tissue Org. Cult. 59 (1999) 65-69.
33. M. Caboche, Nitrogen, carbohydrate and zinc requirements for the efficient induction of shoot morphogenesis from protoplast-derived colonies of Nicotiana plumbaginifolia, Plant Cell Tissue Org. Cult. 8 (1987) 197-206.
34. S. Oka, K. Ohyama, Plant regeneration from leaf mesophyll protoplasts of Broussonetia kazinoki Sieb (paper mulberry), J. Plant Physiol. 119 (1985) 455-460.
35. N. Dorion, H. Ben Jouira, P. Danthu, C. Bigot, Regeneration of plants from protoplasts of Ulmus species (Elms), Biotechnol. Agric. Forest 29 (1994) 172-190.
36. C.H. Bornman, T.C. Vogelman, Effect of rigidity of gel medium on benzyladenine-induced adventitious bud formation and vitrification in vitro in Picea abies, Physiol. Plant 61 (1984) 505-512.
37. H. Saito, M. Nakano, Plant regeneration from suspension cultures of Hosta sieboldiana, Plant Cell Tissue Org. Cult. 71 (2002) 23-28.
38. A.L. Moyne, F. Souq, L.H. Yean, S.C. Brown, M. Boulay, B.S. Sangwan-Norreel, Relationship between cell ploidy and regeneration capacity of long term Rosa hybrida cultures, Plant Sci. 93 (1993) 159-168.