Graphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells

Nature Communications

Volumn 6, Issue , 2015, Pages

  Wojcik, Michal ^a   Hauser, Margaret ^a   Li, Wan ^a   Moon, Seonah ^a   Xu, Ke ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; GRAPHENE; GRAPHITE;

ANIMAL; CORRELATION; CYTOLOGY; ELECTRON MICROSCOPY; FLUORESCENCE SPECTROSCOPY; SAMPLING;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL FUNCTION; CELL STRUCTURE; CELLS; CONTROLLED STUDY; CORRELATED SUPER RESOLUTION MICROSCOPY; CYTOSKELETON; ELECTRON MICROSCOPY; FEMALE; HUMAN; HUMAN CELL; MICROSCOPY; NONHUMAN; SINGLE CELL ANALYSIS; THERMAL CONDUCTIVITY; VACUUM; WET CELL; WHOLE CELL; ANIMAL; CHLOROCEBUS AETHIOPS; COS CELL LINE; FLUORESCENCE IMAGING; HELA CELL LINE; PROCEDURES; RAMAN SPECTROMETRY; SCANNING ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALIA;

ACTIN CYTOSKELETON; ANIMALS; CELLS; CERCOPITHECUS AETHIOPS; COS CELLS; GRAPHITE; HELA CELLS; HUMANS; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; OPTICAL IMAGING; SPECTRUM ANALYSIS, RAMAN;

EID: 84931275463     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms8384     Document Type: Article

References (36)

1. Small, J. V., Rottner, K., Hahne, P., Anderson, K. I. Visualising the actin cytoskeleton. Microsc. Res. Tech. 47, 3-17 (1999).
2. Svitkina, T. Electron microscopic analysis of the leading edge in migrating cells. Methods Cell Biol. 79, 295-319 (2007).
3. Schatten, H., Pawley, J. B. (eds) Biological Low-Voltage Scanning Electron Microscopy. (Springer, 2007).
4. Watanabe, S. et al. Protein localization in electron micrographs using fluorescence nanoscopy. Nat. Methods 8, 80-84 (2011).
5. Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642-1645 (2006).
6. Kopek, B. G., Shtengel, G., Xu, C. S., Clayton, D. A., Hess, H. F. Correlative 3D super resolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes. Proc. Natl Acad. Sci. USA 109, 6136-6141 (2012).
7. Sochacki, K. A., Shtengel, G., van Engelenburg, S. B., Hess, H. F., Taraska, J. W. Correlative super-resolution fluorescence and metal-replica transmission electron microscopy. Nat. Methods 11, 305-308 (2014).
8. Kourkoutis, L. F., Plitzko, J. M., Baumeister, W. Electronmicroscopy of biological materials at the nanometer scale. Annu. Rev. Mater. Res. 42, 33-58 (2012).
9. de Jonge, N., Ross, F. M. Electron microscopy of specimens in liquid. Nature Nanotechnol. 6, 695-704 (2011).
10. Thiberge, S. et al. Scanning electron microscopy of cells and tissues under fully hydrated conditions. Proc. Natl Acad. Sci. USA 101, 3346-3351 (2004).
11. de Jonge, N., Peckys, D. B., Kremers, G. J., Piston, D. W. Electron microscopy of whole cells in liquid with nanometer resolution. Proc. Natl Acad. Sci. USA 106, 2159-2164 (2009).
12. Nishiyama, H. et al. Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film. J. Struct. Biol. 169, 438-449 (2010).
13. Peckys, D. B., Mazur, P., Gould, K. L., de Jonge, N. Fully hydrated yeast cells imaged with electron microscopy. Biophys. J. 100, 2522-2529 (2011).
14. Ring, E. A., Peckys, D. B., Dukes, M. J., Baudoin, J. P., de Jonge, N. Silicon nitride windows for electron microscopy of whole cells. J. Microsc. 243, 273-283 (2011).
15. Geim, A. K., Novoselov, K. S. The rise of graphene. Nat. Mater 6, 183-191 (2007).
16. Bunch, J. S. et al. Impermeable atomic membranes from graphene sheets. Nano Lett. 8, 2458-2462 (2008).
17. Stolyarova, E. et al. Observation of graphene bubbles and effective mass transport under graphene films. Nano Lett. 9, 332-337 (2009).
18. Chen, S. et al. Oxidation resistance of graphene-coated Cu and Cu/Ni alloy. ACS Nano 5, 1321-1327 (2011).
19. Berry, V. Impermeability of graphene and its applications. Carbon N. Y. 62, 1-10 (2013).
20. Xu, K., Cao, P. G., Heath, J. R. Graphene visualizes the first water adlayers on mica at ambient conditions. Science 329, 1188-1191 (2010).
21. Cao, P. G., Xu, K., Varghese, J. O., Heath, J. R. Atomic force microscopy characterization of room-temperature adlayers of small organic molecules through graphene templating. J. Am. Chem. Soc. 133, 2334-2337 (2011).
22. Cao, P. G., Varghese, J. O., Xu, K., Heath, J. R. Visualizing local doping effects of individual water clusters on gold(111)-supported graphene. Nano Lett. 12, 1459-1463 (2012).
23. Meyer, J. C., Girit, C. O., Crommie, M. F., Zettl, A. Imaging and dynamics of light atoms and molecules on graphene. Nature 454, 319-322 (2008).
24. Nair, R. R. et al. Graphene as a transparent conductive support for studying biological molecules by transmission electron microscopy. Appl. Phys. Lett. 97, 153102 (2010).
25. Yuk, J. M. et al. High-resolution EM of colloidal nanocrystal growth using graphene liquid cells. Science 336, 61-64 (2012).
26. Chen, Q. et al. 3D motion of DNA-Au nanoconjugates in graphene liquid cell electron microscopy. Nano Lett. 13, 4556-4561 (2013).
27. Wang, C. H., Qiao, Q., Shokuhfar, T., Klie, R. F. High-resolution electron microscopy and spectroscopy of ferritin in biocompatible graphene liquid cells and graphene sandwiches. Adv. Mater. 26, 3410-3414 (2014).
28. Mohanty, N., Fahrenholtz, M., Nagaraja, A., Boyle, D., Berry, V. Impermeable graphenic encasement of bacteria. Nano Lett. 11, 1270-1275 (2011).
29. Li, Y. F. et al. Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites. Proc. Natl Acad. Sci. USA 110, 12295-12300 (2013).
30. Ferrari, A. C. et al. Raman spectrum of graphene and graphene layers. Phys. Rev. Lett. 97, 187401 (2006).
31. Xu, K., Babcock, H. P., Zhuang, X. Dual-objective STORM reveals threedimensional filament organization in the actin cytoskeleton. Nat. Methods 9, 185-188 (2012).
32. Rust, M. J., Bates, M., Zhuang, X. W. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3, 793-795 (2006).
33. Huang, B., Wang, W. Q., Bates, M., Zhuang, X. W. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science 319, 810-813 (2008).
34. Dempsey, G. T., Vaughan, J. C., Chen, K. H., Bates, M., Zhuang, X. Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging. Nat. Methods 8, 1027-1036 (2011).
35. Li, X. S. et al. Large-area synthesis of high-quality and uniform graphene films on copper foils. Science 324, 1312-1314 (2009).
36. Nair, R. R. et al. Fine structure constant defines visual transparency of graphene. Science 320, 1308-1308 (2008).