Enhanced liver functions of HepG2 cells in the alginate/xyloglucan scaffold

Biotechnology Letters

Volumn 37, Issue 1, 2015, Pages 235-240

  Deng, Xiaojie ^a   Cao, Yu ^a   Yan, Hao ^a   Yang, Jihong ^a   Xiong, Guomei ^a   Yao, Hanchao ^a   Qi, Chao ^a  

^a Central China of Normal University   (China)

Author keywords

Albumin synthesis; Ammonia elimination; Encapsulation; HepG2 cell line; Liver tissue engineering; Xyloglucan

Indexed keywords

AMMONIA; CELL CULTURE; CELL PROLIFERATION; CELLS; CYTOLOGY; ENCAPSULATION; GENE EXPRESSION; POLYMERS; SCAFFOLDS (BIOLOGY); TISSUE; TISSUE ENGINEERING;

EPITHELIAL CADHERIN; EXTRACELLULAR MATRICES; HEPG2 CELL LINE; LIVER TISSUE ENGINEERING; LIVER-SPECIFIC FUNCTIONS; THREE DIMENSIONAL SCAFFOLD; THREE DIMENSIONAL SPACE; XYLOGLUCANS;

CELL ENGINEERING;

ALGINIC ACID; AMMONIA; GLUCAN; GLUCURONIC ACID; HEXURONIC ACID; XYLAN; XYLOGLUCAN;

CELL ADHESION; CELL PROLIFERATION; CELL SHAPE; CHEMISTRY; DRUG EFFECTS; HEPG2 CELL LINE; HUMAN; LIVER; METABOLISM; PROCEDURES; TISSUE ENGINEERING; TISSUE SCAFFOLD;

ALGINATES; AMMONIA; CELL ADHESION; CELL PROLIFERATION; CELL SHAPE; GLUCANS; GLUCURONIC ACID; HEP G2 CELLS; HEXURONIC ACIDS; HUMANS; LIVER; TISSUE ENGINEERING; TISSUE SCAFFOLDS; XYLANS;

EID: 84930905939     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-014-1663-6     Document Type: Article

References (14)

1. Bozza A et al (2014) Neural differentiation of pluripotent cells in 3D alginate-based cultures. Biomaterials 35(16):4636–4645
2. Cho CS, Hoshiba T, Harada I, Akaike T (2007) Regulation of hepatocyte behaviors by galactose-carrying polymers through receptor-mediated mechanism. React Funct Polym 67:1301–1310
3. Glicklis R, Shapiro L, Agbaria R, Merchuk JC, Cohen S (2000) Hepatocyte behavior within three-dimensional porous alginate scaffolds. Biotechnol Bioeng 67:344–353
4. Guo XL et al (2003) Morphology and metabolism of Ba-alginate-encapsulated hepatocytes with galactosylated chitosan and poly(vinyl alcohol) as extracellular matrices. J Biomat Sci-Polym E 14:551–565
5. Klöck G, Pfeffermann A, Ryser C, Gröhn P, Kuttler B, Hahn HJ, Zimmermann U (1997) Biocompatibility of mannuronic acid-rich alginates. Biomaterials 18:707–713
6. Knowles BB, Aden DP (1983) Human hepatoma derived cell line, process for preparation there of, and uses therefor. U.S. Patent 4393133, Jul. 12
7. Koide N et al (1990) Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp Cell Res 186:227–235
8. Liao YT, Liu CH, Yu J, Wu KC (2014) Liver cancer cells: targeting and prolonged-release drug carriers consisting of mesoporous silica nanoparticles and alginate microspheres. Int J Nanomed 5(9):2767–2778
9. Piechocki MP, Toti RM, Fernstrom MJ, Burk RD, Ruch RJ (2000) Liver cell-specific transcriptional regulation of connexin32. Biochim Biophys Acta 1491:107–122
10. Schardt C, Rotsch M, Erbil C, Göke R, Richter G, Havemann K (1993) Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp Cell Res 204:22–29
11. Seo SJ, Park IK, Yoo MK, Shirakawa M, Akaike T, Cho CS (2004) Xyloglucan as a synthetic extracellular matrix for hepatocyte attachment. J Biomat Sci-Polym E 15:1375–1387
12. Seo SJ, Akaike T, Choi YJ, Shirakawa M, Kang IK, Cho CS (2005) Alginate microcapsules prepared with xyloglucan as a synthetic extracellular matrix for hepatocyte attachment. Biomaterials 26:3607–3615
13. York WS, van Halbeek H, Darvill AG, Albersheim P (1990) Structural analysis of xyloglucan oligosaccharides by 1H-n.m.r. spectroscopy and fast-atom-bombardment mass spectrometry. Carbohyd Res 200:9–31
14. Yuan L, Yan YH (2007) Recent advances in degradable biomaterials in liver tissue engineering. Chem Intermed 1:1–4