Functional expression and characterization of recombinant NADPHP450 reductase from Malassezia globosa

Journal of Microbiology and Biotechnology

Volumn 22, Issue 1, 2012, Pages 141-146

  Lee, Hwayoun ^a   Park, Hyoung Goo ^a   Lim, Young Ran ^a   Lee, Im Soon ^a   Kim, Beom Joon ^b   Seong, Cheul Hun ^b   Chun, Young Jin ^b   Kim, Donghak ^a  

^a Konkuk University   (South Korea)

^b Chung Ang University   (South Korea)

Author keywords

7 ethoxyresorufin, coumarin; Cytochrome P450; Malassezia globosa; NADPH; Reductase

Indexed keywords

COUMARIN; CYTOCHROME C; NITROBLUE TETRAZOLIUM; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; MALASSEZIA GLOBOSA; NONHUMAN; OXIDATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SEQUENCE ALIGNMENT; SKIN DISEASE;

FUNGI; MALASSEZIA GLOBOSA;

EID: 84930481876     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1107.07058     Document Type: Article

References (20)

1. Cafarchia, C. and D. Otranto. 2008. The pathogenesis of Malassezia yeasts. Parassitologia 50: 65-67.
2. Gemmer, C. M., Y. M. DeAngelis, B. Theelen, T. Boekhout, and T. L. Dawson Jr. 2002. Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to dandruff microbiology. J. Clin. Microbiol. 40: 3350-3357.
3. Guengerich, F. P. 2001. Analysis and characterization of enzymes and nucleic acids, pp. 1625-1687. In A. W. Hayes (ed.). Principles and Methods of Toxicology, 4 Ed. Taylor & Francis, Philadelphia.
4. Guengerich, F. P., M. V. Martin, C. D. Sohl, and Q. Cheng. 2009. Measurement of cytochrome P450 and NADPHcytochrome P450 reductase. Nat. Protoc. 4: 1245-1251.
5. Guillot, J., S. Hadina, and E. Gueho. 2008. The genus Malassezia: Old facts and new concepts. Parassitologia 50: 77-79.
6. Han, S., C.-Y. Eun, J.-S. Han, Y.-J. Chun, D.-H. Kim, C. H. Yun, and D. Kim. 2009. Self-sufficient catalytic system of human cytochrome P450 4A11 and NADPH-P450 reductase. Biomol. Ther. 72: 156-161.
7. Janik, M. P. and M. P. Heffernam. 2008. Yeast Infections: Candidiasis, pityriasis versicolor, pp. 1822-1830. In K. Wolff, et al. (eds.). Fitzpatrick's Dermatology in General Medicine, 7th Ed. McGraw-Hill, New York.
8. Kim, D. and F. P. Guengerich. 2004. Selection of human cytochrome P450 1A2 mutants with enhanced catalytic activity for heterocyclic amine N-hydroxylation. Biochemistry 43: 981-988.
9. Kim, D., Y. R. Lim, S. O. Ohk, B. J. Kim, and Y. J. Chun. 2011. Functional expression and characterization of CYP51 from dandruff-causing Malassezia globosa. FEMS Yeast Res. 11: 80-87.
10. Kim, D., Z. L. Wu, and F. P. Guengerich. 2005. Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis. J. Biol. Chem. 280: 40319-40327.
11. Kim, D., E. T. Yukl, P. Moenne-Loccoz, and P. R. Ortiz de Montellano. 2006. Fungal heme oxygenases: Functional expression and characterization of Hmx1 from Saccharomyces cerevisiae and CaHmx1 from Candida albicans. Biochemistry 45: 14772-14780.
12. Lamb, D. C., Y. Kim, L. V. Yermalitskaya, V. N. Yermalitsky, G. I. Lepesheva, S. L. Kelly, et al. 2006. A second FMN binding site in yeast NADPH-cytochrome P450 reductase suggests a mechanism of electron transfer by diflavin reductases. Structure 14: 51-61.
13. Lee, E. C., S. O. Ohk, B. Y. Suh, N. H. Park, B. J. Kim, D. Kim, and Y.-J. Chun. 2010. Cloning and expression in Pichia pastoris of a new cytochrome P450 gene from a Dandruffcausing Malassezia globosa. Toxicol. Res. 26: 47-52.
14. Lee, Y. W., H. J. Byun, B. J. Kim, D. H. Kim, Y. Y. Lim, J. W. Lee, et al. 2011. Distribution of Malassezia species on the scalp in Korean seborrheic dermatitis patients. Ann. Dermatol. 23: 156-161.
15. Notredame, C., D. G. Higgins, and J. Heringa. 2000. T-Coffee: A novel method for fast and accurate multiple sequence alignment. J. Mol. Biol. 302: 205-217.
16. Paine, M. J. I., N. S. Scrutton, A. W. Munro, A. Gutierrez, G. C. K. Roberts, and C. R. Wolf. 2005. Electron transfer partners of cytochrome P450, pp. 115-148. In P. R. Ortiz de Montellano (ed.). Cytochrome P450: Structure, Mechanism, and Biochemistry, 3 Ed. Plenum Press, New York.
17. Park, H. G., Y. R. Lim, C. Y. Eun, S. Han, J. S. Han, K. S. Cho, et al. 2010. Candida albicans NADPH-P450 reductase: Expression, purification, and characterization of recombinant protein. Biochem. Biophys. Res. Commun. 396: 534-538.
18. Vermilion, J. L., D. P. Ballou, V. Massey, and M. J. Coon. 1981. Separate roles for FMN and FAD in catalysis by liver microsomal NADPH-cytochrome P-450 reductase. J. Biol. Chem. 256: 266-277.
19. Xu, J., C. W. Saunders, P. Hu, R. A. Grant, T. Boekhout, E. E. Kuramae, et al. 2007. Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens. Proc. Natl. Acad. Sci. USA 104: 18730-18735.
20. Zisova, L. G. 2009. Malassezia species and seborrheic dermatitis. Folia Med. (Plovdiv) 51: 23-33.