Golgi isolation

Cold Spring Harbor Protocols

Volumn 2015, Issue 6, 2015, Pages 562-567

  Tang, Danming ^a   Wang, Yanzhuang ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA N ACETYLGLUCOSAMINYLGLYCOPEPTIDE BETA 1,4 GALACTOSYLTRANSFERASE; BETA N ACETYLHEXOSAMINIDASE; LIPID; N ACETYLLACTOSAMINE SYNTHASE; PROTEIN;

ANIMAL TISSUE; ARTICLE; CELL SPECIFICITY; ELECTRON MICROSCOPY; FEMALE; GOLGI COMPLEX; ISOLATION PROCEDURE; LIVER HOMOGENATE; MATHEMATICAL PARAMETERS; NONHUMAN; PROTEOMICS; RAT; REFRACTOMETER; SCINTILLATION; ANIMAL; CELL FRACTIONATION; DENSITY GRADIENT CENTRIFUGATION; ENZYMOLOGY; LIVER; METABOLISM; PROCEDURES; SPRAGUE DAWLEY RAT; ULTRASTRUCTURE;

RATTUS;

ANIMALS; CELL FRACTIONATION; CENTRIFUGATION, DENSITY GRADIENT; FEMALE; GOLGI APPARATUS; LIPIDS; LIVER; N-ACETYLLACTOSAMINE SYNTHASE; PROTEINS; RATS; RATS, SPRAGUE-DAWLEY;

EID: 84930244977     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot075911     Document Type: Article

References (18)

1. Balch WE, Dunphy WG, Braell WA, Rothman JE. 1984. Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine. Cell 39: 405–416.
2. Bell AW, Ward MA, Blackstock WP, Freeman HN, Choudhary JS, Lewis AP, Chotai D, Fazel A, Gushue JN, Paiement J, et al. 2001. Proteomics characterization of abundant Golgi membrane proteins. J Biol Chem 276: 5152–5165.
3. Chen JL, Xu W, Stamnes M. 2005. In vitro reconstitution of ARF-regulated cytoskeletal dynamics on Golgi membranes. Methods Enzymol 404: 345–358.
4. Chen X, Simon ES, Xiang Y, Kachman M, Andrews PC, Wang Y. 2010. Quantitative proteomics analysis of cell cycle regulated Golgi disassembly and reassembly. J Biol Chem 285: 7197–7207.
5. Chen X, Andrews PC, Wang Y. 2012. Quantitative analysis of liver Golgi proteome in the cell cycle. Methods Mol Biol 909: 125–140.
6. Fleischer B, Fleischer S, Ozawa H. 1969. Isolation and characterization of Golgi membranes from bovine liver. J Cell Biol 43: 59–79.
7. Fullerton AT, Bau MY, Conrad PA, Bloom GS. 1998. In vitro reconstitution of microtubule plus end-directed, GTPγS-sensitive motility of Golgi membranes. Mol Biol Cell 9: 2699–2714.
8. Gilchrist A, Au CE, Hiding J, Bell AW, Fernandez-Rodriguez J, Lesimple S, Nagaya H, Roy L, Gosline SJ, Hallett M, et al. 2006. Quantitative proteomics analysis of the secretory pathway. Cell 127: 1265–1281.
9. Hino Y, Asano A, Sato R, Shimizu S. 1978. Biochemical studies of rat liver Golgi apparatus. I. Isolation and preliminary characterization. J Biochem Tokyo 83: 909–923.
10. Hui N, Nakamura N, Slusarewicz P, Warren G. 1998. Purification of rat liver Golgi stacks. In Cell biology: A laboratory handbook (ed. Celis J), pp. 46–55. Academic, San Diego.
11. Landegren U. 1984. Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens. J Immunol Methods 67: 379–388.
12. Leelavathi DE, Estes LW, Feingold DS, Lombardi B. 1970. Isolation of a Golgi-rich fraction from rat liver. Biochim Biophys Acta 211: 124–138.
13. Misteli T, Warren G. 1995. Mitotic disassembly of the Golgi apparatus in vivo. J Cell Sci 108: 2715–2727.
14. Rabouille C, Misteli T, Watson R, Warren G. 1995. Reassembly of Golgi stacks from mitotic Golgi fragments in a cell-free system. J Cell Biol 129: 605–618.
15. Sottocasa GL, Kuylenstierna B, Ernster L, Bergstrand A. 1967. An electrontransport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol 32: 415–438.
16. Tang D, Xiang Y, Wang Y. 2010. Reconstitution of the cell cycle–regulated Golgi disassembly and reassembly in a cell-free system. Nat Protoc 5: 758–772.
17. Wang Y. 2008. Golgi: Methods for preparation. eLS doi: 10.1002/9780470015902.a0002590.pub2.
18. Wang Y, Taguchi T, Warren G. 2006. Purification of rat liver golgi stacks. In Cell biology: A laboratory handbook, 3rd ed (ed. Celis J), pp. 33–39. Elsevier Science (USA), San Diego.