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Volumn 114, Issue 19, 2015, Pages

Concentrating genomic length DNA in a microfabricated array

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA SEQUENCES; ELASTIC MODULI; GENES; MICROFABRICATION; MOLECULES; SHEAR FLOW; SHEAR STRAIN;

EID: 84930226812     PISSN: 00319007     EISSN: 10797114     Source Type: Journal    
DOI: 10.1103/PhysRevLett.114.198303     Document Type: Article
Times cited : (34)

References (40)
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    • See Supplemental Material at for details on the sample preparation, the parameters of the device, the statistics of bumping, and a description of the movies recorded.
    • See Supplemental Material at http://link.aps.org/supplemental/10.1103/PhysRevLett.114.198303 for details on the sample preparation, the parameters of the device, the statistics of bumping, and a description of the movies recorded.
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    • Staining with one YOYO-1 molecule per 10 base pairs gives a 12% extension of the contour length [29].
    • Staining with one YOYO-1 molecule per 10 base pairs gives a 12% extension of the contour length [29].
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    • The thermal blob size of DNA, within which self-avoidance is not felt, is [31] (Equation presented), where (Equation presented) is the excluded volume of a Kuhn segment [see below Eq. (3)]. As (Equation presented) is comparable to the radius of gyration (Equation presented), the freely jointed chain model gives a reasonable approximation for the size of the molecule.
    • The thermal blob size of DNA, within which self-avoidance is not felt, is [31] (Equation presented), where (Equation presented) is the excluded volume of a Kuhn segment [see below Eq. (3)]. As (Equation presented) is comparable to the radius of gyration (Equation presented), the freely jointed chain model gives a reasonable approximation for the size of the molecule.
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    • In Ref. [17] they use PEG-20000, not PEG-8000 as in the present study.
    • In Ref. [17] they use PEG-20000, not PEG-8000 as in the present study.
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    • In bulk the dominant state of DNA in the presence of condensing agents is rodlike or globular, but it can enter a toroidal state if the addition of condensing agents is carefully tuned. The toroidal state is also observed in single-molecule experiments with optical tweezers. For details, see Ref. [38] and references therein. A recent study with optical tweezers shows that PEG might also induce a toroidal state [39].
    • In bulk the dominant state of DNA in the presence of condensing agents is rodlike or globular, but it can enter a toroidal state if the addition of condensing agents is carefully tuned. The toroidal state is also observed in single-molecule experiments with optical tweezers. For details, see Ref. [38] and references therein. A recent study with optical tweezers shows that PEG might also induce a toroidal state [39].


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