Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR Veterinary Research

BMC Research Notes

Volumn 8, Issue 1, 2015, Pages

  Wareth, Gamal ^a,b,c   Melzer, Falk ^a   Tomaso, Herbert ^a   Roesler, Uwe ^b   Neubauer, Heinrich ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

^c Benha University   (Egypt)

Author keywords

Brucella abortus; Cross species transmission; Real time PCR; Small ruminants

Indexed keywords

ANIMALIA; BOS; BRUCELLA; BRUCELLA MELITENSIS BIOVAR ABORTUS; BUBALUS; CAMELIDAE; CAPRA HIRCUS; OVIS ARIES;

VIRUS DNA;

ABORTION, VETERINARY; ANIMAL; BOVINE; BRUCELLA ABORTUS; BRUCELLOSIS; BRUCELLOSIS, BOVINE; BUFFALO; EGYPT; FEMALE; GENETICS; GOAT; GOAT DISEASES; MICROBIOLOGY; PATHOGENICITY; PREDICTIVE VALUE; PREGNANCY; REAL TIME POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASES; TRANSMISSION; VETERINARY;

ABORTION, VETERINARY; ANIMALS; BRUCELLA ABORTUS; BRUCELLOSIS; BRUCELLOSIS, BOVINE; BUFFALOES; CATTLE; DNA, VIRAL; EGYPT; FEMALE; GOAT DISEASES; GOATS; PREDICTIVE VALUE OF TESTS; PREGNANCY; REAL-TIME POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASES;

EID: 84930156500     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/s13104-015-1173-1     Document Type: Article

References (31)

1. Aparicio ED (2013) Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev sci tech Off int Epiz 32(1):53-60
2. Corbel M. Brinley M.W. Genus Brucella 1984 N.R. Krieg J.G. Holt (eds)
3. Foster G, Osterman B, Godfroid J, Jacques I, Cloeckaert A (2007) Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol 57:2688-2693
4. Scholz H, Hubalek Z, Sedlácek I, Vergnaud G, Tomaso H, Al Dahouk S et al (2008) Brucella microti sp. nov. isolated from the common vole Microtus arvalis. Int J Syst Evol Microbiol 58:375-382
5. Scholz H, Nöckler K, Gollner C (2010) Brucella inopinata sp. nov., isolated from a breast implant infection. Int J Syst Evol Microbiol 60:801-808
6. Whatmore A, Davison N, Cloeckaert A, Al Dahouk S, Zygmunt M, Brew S et al (2014) Brucella papionis sp. nov. isolated from baboons (Papio spp.). Int J Syst Evol Microbiol 64(Pt 12):4120-4128. doi: 10.1099/ijs.0.065482-0 (Epub 2014 Sep 21)
7. Holt HR, Eltholth MM, Hegazy YM, El-Tras WF, Tayel AA, Guitian J (2011) Brucella spp. infection in large ruminants in an endemic area of Egypt: cross-sectional study investigating seroprevalence, risk factors and livestock owner's knowledge, attitudes and practices (KAPs). BMC Public Health 11:341. doi: 10.1186/1471-2458-11-341
8. Samaha H, Al-Rowaily M, Khoudair RM, Ashour HM (2008) Multicenter study of brucellosis in Egypt. Emerg Infect Dis 14(12):1916-1918
9. El-Tras W, Tayel A, Eltholth M, Guitian J (2010) Brucella infection in fresh water fish: evidence for natural infection of Nile catfish, Clarias gariepinus, with Brucella melitensis. Vet Microbiol 141:321-325
10. Refai M (2002) Incidence and control of brucellosis in the Near East region. Vet Microbiol 90:81-110
11. El-Seedy F, Radwan A, El-Shabrawy M (2000) Serological and bacteriological investigations on Brucella infection in one humped camels (Camelus dromedarius) in Egypt. Vet Med J Giza 48:83-89
12. Hamdy MER, Amin AS (2002) Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Vet J 163:299-305
13. Wareth G, Hikal A, Refai M, Melzer F, Roesler U, Neubauer H (2014) Animal brucellosis in Egypt. J Infect Dev Ctries 8(11):1365-1373. doi: 10.3855/jidc.4872
14. Probert WS, Schrader KN, Khuong NY, Bystrom SL, Graves MH (2004) Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J Clin Microbiol 42(3):1290-1293
15. Bercovich Z (1998) Maintenance of Brucella Abortus-free herds: a review with emphasis on the epidemiology and the problems in diagnosing brucellosis in areas of low prevalence. Vet Q 20(3):81-88
16. Poester FP, Nielsen K, Samartino LE, Yu WL (2010) Diagnosis of brucellosis. Open Vet Sci 4:46-60
17. Marianelli C, Martucciello A, Tarantino M, Vecchio R, Iovane G, Galiero G (2008) Evaluation of molecular methods for the detection of Brucella species in water buffalo milk. J Dairy Sci 91:3779-3786
18. Junqueira JD, Rosinha G, Carvalho C, Oliveira C, Sanches C, Lima-Ribeiro A (2013) Detection of Brucella spp. DNA in the semen of seronegative bulls by polymerase chain reaction. Transbound Emerg Dis 60(4):376-377
19. Martirosyan A, Moreno E, Gorvel J-P (2011) An evolutionary strategy for a stealthy intracellular Brucella pathogen. Immunol Rev 240(1):211-234
20. Gorham SL, Enright FM, Snider TG 3rd, Roberts ED (1986) Morphologic lesions in Brucella abortus infected ovine fetuses. Vet Pathol 23(3):331-332
21. Kreeger T, Cook W, Edwards W, Cornish T (2004) Brucellosis in captive Rocky Mountain bighorn sheep (Ovis canadensis) caused by Brucella abortus biovar 4. J Wildl Dis 40(2):311-315
22. Ocholi R, Kwaga J, Ajogi I, Bale J (2005) Abortion due to Brucella abortus in sheep in Nigeria. Rev Sci Tech 24(3):973-979
23. Okoh A (1980) Abortion in sheep near Kano, Nigeria. Trop Anim Health Prod 12(1):11-14
24. Behroozikhah A, Bagheri NR, Amiri K, Bahonar A (2012) Identification at biovar level of Brucella isolates causing abortion in small ruminants of Iran. J Pathog. doi: 10.1155/2012/357235
25. Fosgate GT, Diptee MD, Ramnanan A, Adesiyun AA (2011) Brucellosis in domestic water buffalo (Bubalus bubalis) of Trinidad and Tobago with comparative epidemiology to cattle. Trop Anim Health Prod 43(8):1479-1486
26. Gomo C, de Garine-Wichatitsky M, Caron A, Pfukenyi DM (2012) Survey of brucellosis at the wildlife-livestock interface on the Zimbabwean side of the great Limpopo transfrontier conservation area. Trop Anim Health Prod 44(1):77-85
27. Salem A, Hosein H (1990) Brucella strains prevalent in Egypt. Assiut Vet Med J 22:160-163
28. Amin A, Hamdy M, Ibrahim A (2001) Detection of Brucella melitensis in semen using the polymerase chain reaction assay. Vet Microbiol 83:37-44
29. Samaha H, Mohamed TR, Khoudair RM, Ashour HM (2009) Serodiagnosis of brucellosis in cattle and humans in Egypt. Immunobiology 214:223-226
30. Wareth G, Melzer F, Elschner MC, Neubauer H, Roesler U (2014) Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR. J Infect Dev Ctries 8(10):1339-1343. doi: 10.3855/jidc.4847
31. Richomme C, Gauthier D, Fromont E (2006) Contact rates and exposure to inter-species disease transmission in mountain ungulates. Epidemiol Infect 134(1):21-30