Effect of EphA7 silencing on proliferation, invasion and apoptosis in human laryngeal cancer cell lines Hep-2 and AMC-HN-8

Cellular Physiology and Biochemistry

Volumn 36, Issue 2, 2015, Pages 435-445

  Xiang, Cheng ^a   Lv, Yuanjing ^a   Wei, Yanjie ^a   Wei, Jing ^a   Miao, Susheng ^a   Mao, Xionghui ^a   Gu, Xin ^a   Song, Kaibin ^a   Jia, Shenshan ^a  

^a HARBIN MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; EphA7; Invasion; Laryngeal squamous cell carcinoma; Proliferation; siRNA

Indexed keywords

CASPASE 3; EPHRIN RECEPTOR A7; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PROTEIN BAX; PROTEIN BCL 2; PROTEIN KINASE B; SMALL INTERFERING RNA;

AMC HN 8 CELL LINE; APOPTOSIS; ARTICLE; CELL GROWTH; CELL INVASION; CELL MIGRATION; CELL PROLIFERATION; CLINICAL ARTICLE; CONTROLLED STUDY; DOWN REGULATION; GENE OVEREXPRESSION; GENE SILENCING; HEP 2 CELL LINE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LARYNGEAL CANCER CELL LINE; MTT ASSAY; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN FUNCTION; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; TISSUE SECTION; TUNEL ASSAY; UPREGULATION; WESTERN BLOTTING; CARCINOMA, SQUAMOUS CELL; CELL CYCLE; GENE EXPRESSION REGULATION; GENETICS; LARYNGEAL NEOPLASMS; LARYNX; METABOLISM; PATHOLOGY; PREVENTION AND CONTROL; RNA INTERFERENCE; RNAI THERAPEUTICS; TUMOR CELL LINE; TUMOR INVASION;

APOPTOSIS; CARCINOMA, SQUAMOUS CELL; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; LARYNGEAL NEOPLASMS; LARYNX; NEOPLASM INVASIVENESS; RECEPTOR, EPHA7; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNAI THERAPEUTICS; UP-REGULATION;

EID: 84929674094     PISSN: 10158987     EISSN: 14219778     Source Type: Journal    
DOI: 10.1159/000430110     Document Type: Article

References (29)

1. Choong N, Vokes E: Expanding role of the medical oncologist in the management of head and neck cancer. CA Cancer J Clin 2008;58:32-53.
2. Li F, Liu Y, Kan X, Li Y, Liu M, Lu JG: Elevated expression of integrin alphav and beta5 subunit in laryngeal squamous-cell carcinoma associated with lymphatic metastasis and angiogenesis. Pathol Res Pract 2013;209:105-109.
3. Weller P, Bankfalvi A, Gu X, Dominas N, Lehnerdt GF, Zeidler R, Lang S, Brandau S, Dumitru CA: The role of tumour foxp3 as prognostic marker in different subtypes of head and neck cancer. Eur J Cancer 2014;50:1291-1300.
4. Luo HN, Wang ZH, Sheng Y, Zhang Q, Yan J, Hou J, Zhu K, Cheng Y, Xu YL, Zhang XH, Xu M, Ren XY: Mir-139 targets cxcr4 and inhibits the proliferation and metastasis of laryngeal squamous carcinoma cells. Med Oncol 2014;31:789.
5. Janes PW, Griesshaber B, Atapattu L, Nievergall E, Hii LL, Mensinga A, Chheang C, Day BW, Boyd AW, Bastiaens PI, Jorgensen C, Pawson T, Lackmann M: Eph receptor function is modulated by heterooligomerization of a and b type eph receptors. J Cell Biol 2011;195:1033-1045.
6. Aitsebaomo J, Portbury AL, Schisler JC, Patterson C: Brothers and sisters: Molecular insights into arterialvenous heterogeneity. Circ Res 2008;103:929-939.
7. Steinecke A, Gampe C, Zimmer G, Rudolph J, Bolz J: Epha/ephrin a reverse signaling promotes the migration of cortical interneurons from the medial ganglionic eminence. Development 2014;141:460-471.
8. Reissenweber B, Mosch B, Pietzsch J: Experimental hypoxia does not influence gene expression and protein synthesis of eph receptors and ephrin ligands in human melanoma cells in vitro. Melanoma Res 2013;23:85-95.
9. Jang BG, Lee BL, Kim WH: Distribution of lgr5+ cells and associated implications during the early stage of gastric tumorigenesis. PloS One 2013;8:e82390.
10. Yu G, Gao Y, Ni C, Chen Y, Pan J, Wang X, Ding Z, Wang J: Reduced expression of ephb2 is significantly associated with nodal metastasis in chinese patients with gastric cancer. J Cancer Res Clin Oncol 2011;137:73-80.
11. Chukkapalli S, Amessou M, Dilly AK, Dekhil H, Zhao J, Liu Q, Bejna A, Thomas RD, Bandyopadhyay S, Bismar TA, Neill D, Azoulay L, Batist G, Kandouz M: Role of the ephb2 receptor in autophagy, apoptosis and invasion in human breast cancer cells. Exp Cell Res 2014;320:233-246.
12. Batson J, Maccarthy-Morrogh L, Archer A, Tanton H, Nobes CD: Epha receptors regulate prostate cancer cell dissemination through vav2-rhoa mediated cell-cell repulsion. Biol Open 2014;3:453-462.
13. Brantley-Sieders DM, Dunaway CM, Rao M, Short S, Hwang Y, Gao Y, Li D, Jiang A, Shyr Y, Wu JY, Chen J: Angiocrine factors modulate tumor proliferation and motility through epha2 repression of slit2 tumor suppressor function in endothelium. Cancer Res 2011;71:976-987.
14. Miyazaki T, Kato H, Kimura H, Inose T, Faried A, Sohda M, Nakajima M, Fukai Y, Masuda N, Manda R, Fukuchi M, Tsukada K, Kuwano H: Evaluation of tumor malignancy in esophageal squamous cell carcinoma using different characteristic factors. Anticancer Res 2005;25:4005-4011.
15. Huusko P, Ponciano-Jackson D, Wolf M, Kiefer JA, Azorsa DO, Tuzmen S, Weaver D, Robbins C, Moses T, Allinen M, Hautaniemi S, Chen Y, Elkahloun A, Basik M, Bova GS, Bubendorf L, Lugli A, Sauter G, Schleutker J, Ozcelik H, Elowe S, Pawson T, Trent JM, Carpten JD, Kallioniemi OP, Mousses S: Nonsense-mediated decay microarray analysis identifies mutations of ephb2 in human prostate cancer. Nat Genet 2004;36:979-983.
16. Davalos V, Dopeso H, Velho S, Ferreira AM, Cirnes L, Diaz-Chico N, Bilbao C, Ramirez R, Rodriguez G, Falcon O, Leon L, Niessen RC, Keller G, Dallenbach-Hellweg G, Espin E, Armengol M, Plaja A, Perucho M, Imai K, Yamamoto H, Gebert JF, Diaz-Chico JC, Hofstra RM, Woerner SM, Seruca R, Schwartz S Jr, Arango D: High ephb2 mutation rate in gastric but not endometrial tumors with microsatellite instability. Oncogene 2007;26:308-311.
17. Taneja R, Thisse B, Rijli FM, Thisse C, Bouillet P, Dolle P, Chambon P: The expression pattern of the mouse receptor tyrosine kinase gene mdk1 is conserved through evolution and requires hoxa-2 for rhombomerespecific expression in mouse embryos. Dev Biol 1996;177:397-412.
18. Stanic K, Vera A, Gonzalez M, Recabal A, Astuya A, Torrejon M, Montecinos H, Caprile T: Complementary expression of epha7 and sco-spondin during posterior commissure development. Front Neuroanat 2014;8:49.
19. Lee H, Park E, Kim Y, Park S: Ephrina5-epha7 complex induces apoptotic cell death via tnfr1. Mol Cells 2013;35:450-455.
20. Oricchio E, Wendel HG: Mining the cancer genome uncovers therapeutic activity of epha7 against lymphoma. Cell Cycle 2012;11:1076-1080.
21. Tu Y, Wan L, Fan Y, Wang K, Bu L, Huang T, Cheng Z, Shen B: Ischemic postconditioning-mediated mirna-21 protects against cardiac ischemia/reperfusion injury via pten/akt pathway. PloS One 2013;8:e75872.
22. Han G, Wang Y, Bi W, Jia J, Wang W: Microrna-124 functions as a tumor suppressor and indicates prognosis in human osteosarcoma. Exp Ther Med 2015;9:679-684.
23. Sefton M, Nieto MA: Multiple roles of eph-like kinases and their ligands during development. Cell Tissue Res 1997;290:243-250.
24. Hafner C, Schmitz G, Meyer S, Bataille F, Hau P, Langmann T, Dietmaier W, Landthaler M, Vogt T: Differential gene expression of eph receptors and ephrins in benign human tissues and cancers. Clin Chem 2004;50:490-499.
25. Wang J, Li G, Ma H, Bao Y, Wang X, Zhou H, Sheng Z, Sugimura H, Jin J, Zhou X: Differential expression of epha7 receptor tyrosine kinase in gastric carcinoma. Hum Pathol 2007;38:1649-1656.
26. Wang LF, Fokas E, Juricko J, You A, Rose F, Pagenstecher A, Engenhart-Cabillic R, An HX: Increased expression of epha7 correlates with adverse outcome in primary and recurrent glioblastoma multiforme patients. BMC Cancer 2008;8:79.
27. Surawska H, Ma PC, Salgia R: The role of ephrins and eph receptors in cancer. Cytokine Growth Factor Rev 2004;15:419-433.
28. Garcia-Ceca J, Alfaro D, Montero-Herradon S, Zapata AG: Eph/ephrinb signalling is involved in the survival of thymic epithelial cells. Immunol Cell Biol 2013;91:130-138.
29. Figueroa JD, Benton RL, Velazquez I, Torrado AI, Ortiz CM, Hernandez CM, Diaz JJ, Magnuson DS, Whittemore SR, Miranda JD: Inhibition of epha7 up-regulation after spinal cord injury reduces apoptosis and promotes locomotor recovery. J Neurosci Res 2006;84:1438-1451.