Generation, expansion, and differentiation of human pluripotent stem cell (hPSC) derived neural progenitor cells (NPCs)

Methods in Molecular Biology

Volumn 1212, Issue , 2015, Pages 87-102

  Brafman, David A ^a  

^a NONE

Author keywords

Embryoid bodies; Human embryonic stem cell (hESC); Human pluripotent stem cell (hPSC); Neural progenitor cells (NPC); Neuronal differentiation

Indexed keywords

BETA TUBULIN; MICROTUBULE ASSOCIATED PROTEIN 2; NESTIN; TRANSCRIPTION FACTOR SOX1; TRANSCRIPTION FACTOR SOX2;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL EXPANSION; CELL POPULATION; CELL SUSPENSION; CELL THERAPY; EMBRYO; EMBRYOID BODY; HUMAN; HUMAN CELL; MULTIPOTENT STEM CELL; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; CULTURE TECHNIQUE; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; METABOLISM; NERVE CELL;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; EMBRYOID BODIES; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; NEURAL STEM CELLS; NEURONS; PLURIPOTENT STEM CELLS;

EID: 84929648615     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651_2014_90     Document Type: Article

References (10)

1. Lunn JS, Sakowski SA, Hur J, Feldman EL (2011) Stem cell technology for neurodegenerative diseases. Ann Neurol 70:353–361
2. Yoo J, Kim HS, Hwang DY (2012) Stem cells as promising therapeutic options for neurological disorders. J Cell Biochem 114:743–753
3. Lindvall O, Kokaia Z (2006) Stem cells for the treatment of neurological disorders. Nature 441:1094–1096
4. Lindvall O, Kokaia Z (2010) Stem cells in human neurodegenerative disorders–time for clinical translation? J Clin Invest 120:29–40
5. Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 27:275–280
6. Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L (2008) Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev 22:152–165
7. Shin S, Mitalipova M, Noggle S, Tibbitts D, Venable A, Rao R, Stice SL (2006) Long-term proliferation of human embryonic stem cellderived neuroepithelial cells using defined adherent culture conditions. Stem Cells 24:125–138
8. Bretzner F, Gilbert F, Baylis F, Brownstone RM (2011) Target populations for first-inhuman embryonic stem cell research in spinal cord injury. Cell Stem Cell 8:468–475
9. Schwartz SD, Hubschman JP, Heilwell G, Franco-Cardenas V, Pan CK, Ostrick RM, Mickunas E, Gay R, Klimanskaya I, Lanza R (2012) Embryonic stem cell trials for macular degeneration: a preliminary report. Lancet 379:713–720
10. Marchetto MC, Winner B, Gage FH (2010) Pluripotent stem cells in neurodegenerative and neurodevelopmental diseases. Hum Mol Genet 19:R71–R76