Comparison of nasopharyngeal aspirate with flocked swab for PCR-detection of respiratory viruses in children

APMIS

Volumn 123, Issue 6, 2015, Pages 473-477

  Tunsjø, Hege Smith ^a   Berg, Are Stuitz ^a   Inchley, Christopher Steven ^a   Røberg, Irmelin Kittelsen ^a   Leegaard, Truls Michael ^a,b  

^a AKERSHUS UNIVERSITY HOSPITAL   (Norway)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

Aspirate; Flocked swab; PCR; Respiratory viruses

Indexed keywords

ARTICLE; CHILD; HUMAN; HUMAN METAPNEUMOVIRUS; HUMAN PARAINFLUENZA VIRUS 1; HUMAN PARAINFLUENZA VIRUS 2; HUMAN PARAINFLUENZA VIRUS 3; HUMAN PARAINFLUENZA VIRUS 4; HUMAN RESPIRATORY SYNCYTIAL VIRUS A; INFLUENZA VIRUS A; INFLUENZA VIRUS A H1N1; INFLUENZA VIRUS B; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NASOPHARYNGEAL ASPIRATION; NASOPHARYNGEAL FLOCKED SWAB; NONHUMAN; NOSE SMEAR; PRIORITY JOURNAL; RESPIRATORY VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS LOAD; COMPARATIVE STUDY; HUMAN RESPIRATORY SYNCYTIAL VIRUS; INFANT; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; NASOPHARYNX; PRESCHOOL CHILD; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; VIROLOGY;

CHILD, PRESCHOOL; HUMANS; INFANT; NASOPHARYNX; REAL-TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL VIRUSES; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIRAL LOAD;

EID: 84929607547     PISSN: 09034641     EISSN: 16000463     Source Type: Journal    
DOI: 10.1111/apm.12375     Document Type: Article

References (16)

1. Bryce J, Boschi-Pinto C, Shibuya K, Black RE. The WHO Child Health Epidemiology Reference Group WHO estimates of the causes of death in children. Lancet 2005;365:1147-52.
2. Nair H, Nokes DJ, Gessner BD, Dherani M, Madhi SA, Singleton RJ, et al. Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis. Lancet 2010;1:375.
3. DeByle C, Bilkow L, Miernyk K, Chikoyak L, Hummel KB, Hennessy T, et al. Comparison of nasopharyngeal flocked swabs and nasopharyngeal wash collection methods for respiratory virus detection in hospitalized children using ral-time polymerase chain reaction. J Virol Methods 2012;185:89-93.
4. Meerhoff TJ, Houben ML, Coenjaerts FEJ, Kimpen JLL, Hofland RW, Schellevis FS, et al. Detection of multiple respiratory pathogens during primary respiratory infection: nasal swab vs nasopharyngeal aspirate using real-time polymerase reaction. EJCMID 2010;29:365-71.
5. Chan KH, Peiris JSM, Lim W, Nicholls JM, Chiu SS. Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children. J Clin Virol 2008;42:65-9.
6. Abu-Diab A, Azzeh M, Ghneim R, Ghneim R, Zoughbi M, Turkuman S, et al. Comparison between pernasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children. J Clin Microbiol 2008;46:2414-7.
7. Ward CL, Dempsey MH, Ring CJA, Kempson RE, Zhang L, Gor D, et al. Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. J Clin Virol 2004;29:179-88.
8. Schulze M, Nitsche A, Schweiger B, Biere B. Diagnostic approach for the differentiation of the pandemic influenza A (H1N1) virus from recent human influenza viruses by realtimePCR. PLoS ONE 2010;5:e9966.
9. Gunson RN, Collins TC, Carman WF. Real-time RT-PCR detection of 12 respiratory viral infection in four triplex reactions. J Clin Virol 2005;33:341-4.
10. Brittain-Long R, Nord S, Olofsson S, Westin J, Anderson LM, Lindh M. Multiplex real-time PCR for detection of respiratory tract infections. J Clin Virol 2008;41:53-6.
11. Tiveljung-Lindell A, RotzeŽn-Ostlund M, Gupta S, Ullstrand R, Grillner L, Zweygberg-Wirgart B, et al. Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses. J Med Virol 2009;81:167-75.
12. Gunson RN, Carman WF. During the summer 2009 outbreak of 'swine flu' in Scotland what respiratory pathogens were diagnosed as H1N1/2009? BMC Infect Dis 2011;11:192.
13. Hu A, Colella M, Tam JS, Rappaport R, Cheng S-M. Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR. J Clin Microbiol 2003;41:149-54.
14. Maertzdorf J, Wang CK, Brown JB, Quinto JD, Chu M, de Graaf M, et al. Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages. J Clin Microbiol 2004;42:981-6.
15. Warisa M, Österbacka R, Lahtib E, Vuorinena T, Ruuskanenb O, Peltolab V. Comparison of sampling methods for the detection of human rhinovirus RNA. J Clin Virol 2013;58:200-4.
16. Jafri HS, Wu X, Makari D, Henrickson KJ. Distribution of respiratory syncytial virus subtypes A and B among infants presenting to the emergency department with lower respiratory tract infection or apnea. Pediatr Infect Dis J 2013;32:335-40.