Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting

BMC Infectious Diseases

Volumn 14, Issue 1, 2014, Pages

  Espírito Santo, Maria Cristina Carvalho ^a,e   Alvarado Mora, Mónica Viviana ^a   Dias Neto, Emmanuel ^a,b   Botelho Lima, Lívia Souza ^a   Moreira, João Paulo ^a   Amorim, Maria ^b   Pinto, Pedro Luiz Silva ^c   Heath, Ashley R ^d   Castilho, Vera Lúcia Pagliusi ^a   Gonçalves, Elenice Messias do Nascimento ^a   Luna, Expedito José de Albuquerque ^a   Carrilho, Flair José ^a   Pinho, João Renato Rebello ^a   Gryschek, Ronaldo Cesar Borges ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b A C CAMARGO CANCER CENTER   (Brazil)

^c INSTITUTO ADOLFO LUTZ   (Brazil)

^d Sigma Custom Products   (United States)

^e Discipline of Pediatrics School of Medicine at the University Center of Volta Redonda   (Brazil)

Author keywords

Brazil epidemiology; Laboratory diagnosis; Low endemicity areas; Schistosomiasis mansoni; TaqMan Real Time PCR system

Indexed keywords

DNA; HELMINTH DNA;

ADULT; ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; CROSS-SECTIONAL STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FECES ANALYSIS; FEMALE; HOFFMAN PONS AND JANER TECHNIQUE; HUMAN; INTERMETHOD COMPARISON; KATO KATZ TECHNIQUE; MAJOR CLINICAL STUDY; MALE; MICROBIOLOGICAL EXAMINATION; MIDDLE AGED; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION SYSTEM; PREDICTIVE VALUE; REAL TIME POLYMERASE CHAIN REACTION; SCHISTOSOMIASIS MANSONI; SENSITIVITY AND SPECIFICITY; YOUNG ADULT; ANIMAL; BLOOD; BRAZIL; ENDEMIC DISEASE; EVALUATION STUDY; FECES; GENETICS; MOLECULAR DIAGNOSIS; PARASITE LOAD; PARASITOLOGY; PREVALENCE; SCHISTOSOMA MANSONI;

ADULT; ANIMALS; BRAZIL; CROSS-SECTIONAL STUDIES; DNA, HELMINTH; ENDEMIC DISEASES; FECES; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; PARASITE LOAD; PREVALENCE; REAL-TIME POLYMERASE CHAIN REACTION; SCHISTOSOMA MANSONI; SCHISTOSOMIASIS MANSONI; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 84929507684     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/s12879-014-0558-4     Document Type: Article

References (46)

1. Chitsulo L, Engels D, Montresor A, Savioli L: The global status of schistosomiasis and its control. Acta Trop 2000, 77:41-51.
2. WHO: Media Centre. Schistosomiasis. Fact sheet no 115. Geneva: World Health Organization; 2012.
3. Brasil. Ministério da Saúde. Secretaria de Vigilância a Saúde: Sistema de Informação do Programa de Controle da Esquistossomose. Sistema de Informação de Agravos em Saúde/2012. Casos confirmados de Esquistossomose, 1995 a 2011. Brasília (DF). Brasil: Grandes Regiões e Unidades Federadas; 2012.
4. Bergquist NR: Schistosomiasis: from risk assessment to control. Trends Parasitol 2002, 18:309-314.
5. Pordeus LC, Aguiar LR, Quinino LRM, Barbosa CS: A ocorrência das formas aguda e crônica da esquistossomose mansônica no Brasil no período de 1997 a 2006: uma revisão de literatura. Epidemiol Serv Saúde 2008, 17:163-175.
6. WHO: Report of Informal Consultation on Schistosomiasis in low Transmission Areas: Control Strategies and Criteria for Elimination, London, 10-13 April 2000. Geneva: World Health Organization; 2001.
7. de Janeiro R: Secretaria de Estado de Saúde e Defesa Civil. Rio de Janeiro: Governo do Estado do Rio de Janeiro. Caderno de Informações em Saúde do Estado do Rio de Janeiro; 2009.
8. Rio de Janeiro. Secretaria Municipal de Saúde: Coordenadoria de Epidemiologia. Barra Mansa. Rio de Janeiro: Documentos retirados do SINANNET/07-05-2008; 2008.
9. Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde: Conselho de Desenvolvimento Social. Brasília (DF): Programa Especial de Controle da Esquistossomose no Brasil; 1976.
10. Ruiz-Tiben E, Hillyer GV, Knight WB, De Rios GI, Woodall JP: Intensity of infection with Schistosoma mansoni: its relationship to the sensitivity and specificity of serologic tests. Am J Trop Med Hyg 1979, 28:230-236.
11. Ebrahim A, El-Morshedy H, Omer E, El-Daly S, Barakat R: Evaluation of the Kato-Katz thick smear and formol ether sedimentation techniques for quantitative diagnosis of Schistosoma mansoni infection. Am J Trop Med Hyg 1997, 57:706-708.
12. Cavalcanti MG, Silva LF, Peralta RH, Barreto MG, Peralta JM: Schistosomiasis in areas of low endemicity: a new era in diagnosis. Trends Parasitol 2013, 29:75-82.
13. de Noya BAA, Guevara RR, Colmenares C, Losada S, Noya O: Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control. Mem Inst Oswaldo Cruz 2006, 101:29-35.
14. Saiki R, Gelfand D, Stoffel S, Scharf S, Higuchi R, Horn G, Mullis K, Erlich H: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988, 239:487-491.
15. Kirkwood B, Sterne JAC: Essential Medical Statistics. 2nd edition. Massachusetts: Blackwell Publishing; 2003.
16. HSMO. Her Majesty's Stationery Office. Office of Public Sector Information (OPSI): Geneva Conventions (Amendment) Act 1995 (c. 27). London; 1995.
17. Kato K, Miura M: Comparative examinations. Jpn J Parasitol 1954, 3:35.
18. Katz N, Chaves A, Pellegrino J: A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 1972, 14:397-400.
19. Hamburger J, Turetski T, Kapeller I, Deresiewicz R: Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by a species-specific probe. Mol Biochem Parasitol 1991, 44:73-80.
20. Espírito-Santo MCC, Alvarado-Mora MV, Pinto PL, Botelho LS, Dias-Neto E, Chieffi PP, Carrilho FJ, Pinho JR, Gryschek RCB: Early Detection of Schistosoma Mansoni Infection by Real Time PCR in Hamster Model-is a Serum Sample Better Than Feces? In Em: XVIII International Congress for Tropical Medicine and Malaria, 2012, Rio de Janeiro. Anais, v. II; 2012:662-663.
21. Espírito-Santo MCC, Alvarado-Mora MV, Pinto PL, Carrilho FJ, Pinho JR, Gryschek RCB: Two sequential PCR amplifications for detection of Schistosoma mansoni in fecal samples with low parasite load. Rev Inst Med Trop Sao Paulo 2012, 54:245-248.
22. Espírito-Santo MCC, Alvarado-Mora MV, Pinto PLS, Carrilho FJ, Pinho JR, Gryschek RCB: Padronização de métodos de extração de DNA para a detecção do Schistosoma mansoni para áreas de baixa endemicidade. In XLVII Congresso da Sociedade Brasileira de Medicina Tropical; 2011, 85. Natal, BR: Anais do XLVII Congresso da Sociedade Brasileira de Medicina Tropical; 2011:41-116.
23. Espírito-Santo MC, Alvarado-Mora MV, Pinto PL, de Brito T, Botelho-Lima L, Heath AR, Amorim MG, Dias-Neto E, Chieffi PP, Pinho JR, Carrilho FJ, Albuquerque Luna EJ, Gryschek RC: Detection of Schistosoma mansoni infection by TaqMan® Real-Time PCR in a hamster model. Exp Parasitol 2014, 143:83-89.
24. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162:156-159.
25. Pontes LA, Dias-Neto E, Rabello A: Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces. Am J Trop Med Hyg 2002, 66:157-162.
26. Doenhoff MJ, Chiodini PL, Hamilton JV: Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies? Trends Parasitol 2004, 20:35-39.
27. Sorgho H, Bahgat M, Poda JN, Song W, Kirsten C, Doenhoff MJ, Zongo I, Ouedraogo JB, Ruppel A: Serodiagnosis of Schistosoma mansoni infections in an endemic area of Burkina Faso: performance of several immunological tests with different parasite antigens. Acta Trop 2005, 93:169-180.
28. Simpson AJ, Dame JB, Lewis FA, McCutchan TF: The arrangement of ribosomal RNA genes in Schistosoma mansoni. Identification of polymorphic structural variants. Eur J Biochem 1984, 139:41-45.
29. van Keulen H, Loverde PT, Bobek LA, Rekosh DM: Organization of the ribosomal RNA genes in Schistosoma mansoni. Mol Biochem Parasitol 1985, 15:215-230.
30. Spotila LD, Rekosh DM, LoVerde PT: Polymorphic repeated DNA element in the genome of Schistosoma mansoni. Mol Biochem Parasitol 1991, 48:117-120.
31. Enk MJ, Lustosa Lima AC, Drummond SC, Schall VT, Coelho PMZ: The impact of the number of fecal samples on the prevalence, the infection intensity and the distribution of the infection with Schistosoma mansoni among a population in an area of low transmission. Acta Trop 2008, 108:222-228.
32. Wichmann D, Poppert S, Thien HV, Clerinx J, Dieckmann S, Jensenius M, Philippe Parola GD, Richter J, Schunk M, Stich A, Zanger P, Burchard GD, Egbert Tannich E: Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis. BMC Infect Dis 2013, 13:55.
33. Gomes AL, Melo FL, Werkhauser RP, Abath FG: Development of a real time polymerase chain reaction for quantitation of Schistosoma mansoni DNA. Mem Inst Oswaldo Cruz 2006, 101:133-136.
34. ten Hove RJ, Verweij JJ, Vereecken K, Polman K, Dieye L, van Lieshout L: Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in fecal samples collected in northern Senegal. Trans R Soc Trop Med Hyg 2008, 102:179-185.
35. Pontes LA, Oliveira MC, Katz N, Dias-Neto E, Rabello A: Comparison of a polymerase chain reaction and the Kato-Katz technique for diagnosing infection with Schistosoma mansoni. Am J Trop Med Hyg 2003, 68:652-656.
36. Gomes LI, Marques LH, Enk MJ, Coelho PM, Rabello A: Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human fecal samples. Mem Inst Oswaldo Cruz 2009, 104:1194-1196.
37. Teesdale CH, Fahringer K, Chistulo L: Egg count variation and sensitivity of a thin smear technique for the diagnosis of Schistosoma mansoni. Trans R Soc Trop Med Hyg 1985, 79:369-373.
38. Engels D, Sinzinkayo E, Gryseels B: Day-to-day egg count fluctuation in Schistosoma mansoni infection and its operational implications. Am J Trop Med Hyg 1996, 54:319-324.
39. Zhou L, Tang J, Zhao Y, Gong R, Lu X, Gong L, Wang Y: A highly sensitive TaqMan real-time PCR assay for early detection of Schistosoma species. Acta Trop 2011, 120:88-94.
40. Oliveira LM, Santos HL, Gonçalves MM, Barreto MG, Peralta JM: Evaluation of polymerase chain reaction as an additional tool for the diagnosis of low-intensity Schistosoma mansoni infection. Diagn Microbiol Infect Dis 2010, 68:416-421.
41. Carneiro TR, Pinheiro MC, de Oliveira SM, Hanemann AL, Queiroz JA, Bezerra FS: Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area. Rev Soc Bras Med Trop 2012, 45:510-513.
42. Wichmann D, Panning M, Quack T, Kramme S, Burchard GD, Grevelding C, Drosten C: Diagnosing schistosomiasis by detection of cell-free parasite DNA in human plasma. PLoS Negl Trop Dis 2009, 3:e422.
43. Cnops L, Tannich E, Polman K, Clerinx J, Van Esbroeck M: Schistosoma real-time PCR as diagnostic tool for international travellers and migrants. Trop Med Int Health 2012, 17:1208-1216.
44. Enk MJ, Oliveira E, Silva G, Rodrigues NB: Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area. PLoS One 2012, 7:e38947.
45. Zhang YY, Luo JP, Liu YM, Wang QZ, Chen JH, Xu MX, Xu JM, Wu J, Tu XM, Wu GL, Zhang ZS, Wu HW: Evaluation of Kato-Katz examination method in three areas with low-level endemicity of schistosomiasis japonica in China: a Bayesian modeling approach. Acta Trop 2009, 112:16-22.
46. Teles HMS: Estudo parasitológico da transmissão e dos impactos da profilaxia da esquistossomose mansônica no município de Bananal. Estado de São Paulo: Brasil. Tese. Universidade Estadual de Campinas, Instituto de Biologia; 2001.