Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo-and heteromeric complexes among hyaluronan synthases HAS1 HAS2, and HAS3

Journal of Biological Chemistry

Volumn 290, Issue 18, 2015, Pages 11479-11490

  Bart, Geneviève ^a   Vico, Nuria Ortega ^b   Hassinen, Antti ^b   Pujol, Francois M ^b   Deen, Ashik Jawahar ^a   Ruusala, Aino ^c   Tammi, Raija H ^a   Squire, Anthony ^d   Heldin, Paraskevi ^c   Kellokumpu, Sakari ^b   Tammi, Markku I ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

^b UNIVERSITY OF OULU   (Finland)

^c LUDWIG INSTITUTE FOR CANCER RESEARCH   (Sweden)

^d UNIVERSITY HOSPITAL ESSEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COBALT COMPOUNDS; ENERGY TRANSFER; ENZYMES; FLUORESCENCE; HYALURONIC ACID;

CO-IMMUNOPRECIPITATION EXPERIMENTS; ENZYME COMPLEXES; FLUORESCENCE RESONANCE ENERGY TRANSFER; GLYCOSYLTRANSFERASES; HETEROMERIC COMPLEXES; HYALURONAN SYNTHASES; HYALURONAN SYNTHESIS; SPECIFIC EFFECTS;

SYNTHESIS (CHEMICAL);

HYALURONAN SYNTHASE 1; HYALURONAN SYNTHASE 1 HYALURONAN SYNTHASE 2 COMPLEX; HYALURONAN SYNTHASE 2; HYALURONAN SYNTHASE 2 HYALURONAN SYNTHASE 2 COMPLEX; HYALURONAN SYNTHASE 2 HYALURONAN SYNTHASE 3 COMPLEX; HYALURONAN SYNTHASE 3; HYALURONIC ACID; ISOENZYME; MULTIENZYME COMPLEX; UNCLASSIFIED DRUG; GLUCURONOSYLTRANSFERASE; HAS1 PROTEIN, HUMAN; HAS2 PROTEIN, HUMAN; HAS3 PROTEIN, HUMAN; PEPTIDE FRAGMENT;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; BLEACHING; CARBOHYDRATE SYNTHESIS; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME LOCALIZATION; FLOW CYTOMETRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GOLGI COMPLEX; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROXIMITY LIGATION ASSAY; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CHLOROCEBUS AETHIOPS; COS 1 CELL LINE; METABOLISM; PROTEIN MULTIMERIZATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN TERTIARY STRUCTURE;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; GLUCURONOSYLTRANSFERASE; HUMANS; HYALURONIC ACID; ISOENZYMES; MICE; PEPTIDE FRAGMENTS; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY;

EID: 84929500835     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M115.640581     Document Type: Article

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