Expression and efficient purification of tag-cleaved active recombinant human insulin-like growth factor-II from Escherichia coli

Biotechnology and Bioprocess Engineering

Volumn 20, Issue 2, 2015, Pages 234-241

  Li, Hongbo ^a,b   Hui, Xiaoyan ^c   Li, Peng ^b   Xu, Aimin ^b,c   Li, Shiwu ^d   Jin, Shouguang ^d   Wu, Donghai ^b  

^a HUAIHUA UNIVERSITY   (China)

^b GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

^c UNIVERSITY OF HONG KONG   (Hong Kong)

^d UNIVERSITY OF FLORIDA   (United States)

Author keywords

enterokinase; Escherichia coli; insulin like growth factor II; protein expression and purification; recombinant protein

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; CELL DEATH; CELL PROLIFERATION; CELL SIGNALING; CLONING; ESCHERICHIA COLI; INSULIN; PURIFICATION;

BACTERIAL CULTURES; BACTERIAL PROTEIN; ENTEROKINASE; FUSION PROTEINS; INSULIN LIKE GROWTH FACTOR II; PROLIFERATION AND APOPTOSIS; PROTEIN EXPRESSIONS; SIGNALING PATHWAYS;

RECOMBINANT PROTEINS;

BACTERIAL PROTEIN; COMPLEMENTARY DNA; DNA FRAGMENT; ENTEROPEPTIDASE; HYBRID PROTEIN; PROTEIN KINASE B; RECOMBINANT ENZYME; RECOMBINANT SOMATOMEDIN B; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL PROLIFERATION; ESCHERICHIA COLI; HUMAN; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT PLASMID; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84929460406     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-014-0562-y     Document Type: Article

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