Mechanism of pyranopterin ring formation in molybdenum cofactor biosynthesis

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 20, 2015, Pages 6347-6352

  Hover, Bradley M ^a   Tonthat, Nam K ^a   Schumacher, Maria A ^a   Yokoyama, Kenichi ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

CPMP synthase; Enzymatic rearrangement; Molybdenum cofactor; Pterin biosynthesis; Radical SAM enzyme

Indexed keywords

ESSENTIAL AMINO ACID; GUANOSINE TRIPHOSPHATE; MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN A; MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN C; PROTEIN; PROTEIN VARIANT; PTERIN DERIVATIVE; PYRANOPTERIN; UNCLASSIFIED DRUG; CARBON; COENZYME; ESCHERICHIA COLI PROTEIN; HYDROLASE; METALLOPROTEIN; MOAC PROTEIN, E COLI; MOLYBDENUM COFACTOR; PTERIDINE DERIVATIVE; S-ADENOSYLMETHIONINE ENZYME MOAA, STAPHYLOCOCCUS AUREUS;

ARTICLE; BIOSYNTHESIS; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; REACTION ANALYSIS; X RAY CRYSTALLOGRAPHY; CHEMICAL STRUCTURE; CHEMISTRY; COENZYME; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR MODEL; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SITE DIRECTED MUTAGENESIS;

CARBON ISOTOPES; COENZYMES; ESCHERICHIA COLI PROTEINS; HYDROLASES; MAGNETIC RESONANCE SPECTROSCOPY; METALLOPROTEINS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PTERIDINES; PTERINS;

EID: 84929441317     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1500697112     Document Type: Article

References (8)

1. Mendel RR, Schwarz G (2011) Molybdenum cofactor biosynthesis in plants and humans. Coord Chem Rev 255:1145-1158.
2. Leimkühler S, Wuebbens MM, Rajagopalan KV (2011) The history of the discovery of the molybdenum cofactor and novel aspects of its biosynthesis in bacteria. Coord Chem Rev 255(9-10):1129-1144.
3. Wuebbens MM, Rajagopalan KV (1993) Structural characterization of a molybdopterin precursor. J Biol Chem 268(18):13493-13498.
4. Wuebbens MM, Rajagopalan KV (1995) Investigation of the early steps of molybdopterin biosynthesis in Escherichia coli through the use of in vivo labeling studies. J Biol Chem 270(3):1082-1087.
5. Reiss J, et al. (1998) Mutations in a polycistronic nuclear gene associated with molybdenum cofactor deficiency. Nat Genet 20(1):51-53.
6. Hänzelmann P, Schindelin H (2004) Crystal structure of the S-adenosylmethionine-dependent enzyme MoaA and its implications for molybdenum cofactor deficiency in humans. Proc Natl Acad Sci USA 101(35):12870-12875.
7. Frey PA, Hegeman AD, Ruzicka FJ (2008) The radical SAM superfamily. Crit Rev Biochem Mol Biol 43(1):63-88.
8. Mehta AP, Abdelwahed SH, Xu H, Begley TP (2014) Molybdopterin biosynthesis: Trapping of intermediates for the MoaA-catalyzed reaction using 2′-deoxyGTP and 2′-chloroGTP as substrate analogues. J Am Chem Soc 136(30):10609-10614.