Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope

Science Translational Medicine

Volumn 7, Issue 286, 2015, Pages

  D'Ambrosio, Michael V ^a   Bakalar, Matthew ^a   Bennuru, Sasisekhar ^b   Reber, Clay ^a   Skandarajah, Arunan ^a   Nilsson, Lina ^a   Switz, Neil ^a,c   Kamgno, Joseph ^d,e   Pion, Sébastien ^d,f   Boussinesq, Michel ^f   Nutman, Thomas B ^b   Fletcher, Daniel A ^a,c  

^a University of California   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d Filariasis and Other Tropical Diseases Research Centre   (Cameroon)

^e UNIVERSITY OF YAOUNDÉ I   (Cameroon)

^f INSTITUT DE RECHERCHE POUR LE DÉVELOPPEMENT   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOMATION; BLOOD SAMPLING; BLOOD SMEAR; CAMEROON; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; HUMAN; IMAGE ANALYSIS; LOIASIS; MICROSCOPE; MOBILE PHONE; MOBILE PHONE MICROSCOPE; MOTION ANALYSIS SYSTEM; NONHUMAN; PARASITE IDENTIFICATION; POINT OF CARE TESTING; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; ALGORITHM; ANIMAL; CENTRAL AFRICA; ELEPHANTIASIS, FILARIAL; IMAGE PROCESSING; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; LOA; MOTION; ONCHOCERCIASIS; POINT OF CARE SYSTEM; PROCEDURES; REPRODUCIBILITY; VIDEO MICROSCOPY;

AFRICA, CENTRAL; ALGORITHMS; ANIMALS; AUTOMATION; CAMEROON; CELL PHONES; ELEPHANTIASIS, FILARIAL; FALSE NEGATIVE REACTIONS; IMAGE PROCESSING, COMPUTER-ASSISTED; LOA; MICROSCOPY, VIDEO; MOTION; ONCHOCERCIASIS; POINT-OF-CARE SYSTEMS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84928975448     PISSN: 19466234     EISSN: 19466242     Source Type: Journal    
DOI: 10.1126/scitranslmed.aaa3480     Document Type: Article

References (21)

1. M. Boussinesq, Loiasis. Ann. Trop. Med. Parasitol. 100, 715-731 (2006).
2. W. G. Metzger, B. Mordmüller, Loa loa-Does it deserve to be neglected? Lancet Infect. Dis. 14, 353-357 (2014).
3. M.-G. Basáñez, S. D. S. Pion, T. S. Churcher, L. P. Breitling, M. P. Little, M. Boussinesq, River blindness: A success story under threat? PLOS Med. 3, e371 (2006).
4. L. M. Zeldenryk, M. Gray, R. Speare, S. Gordon, W. Melrose, The emerging story of disability associated with lymphatic filariasis: A critical review. PLOS Negl. Trop. Dis. 5, e1366 (2011).
5. J. Gardon, N. Gardon-Wendel, Demanga-Ngangue, J. Kamgno, J. P. Chippaux, M. Boussinesq, Serious reactions after mass treatment of onchocerciasis with ivermectin in an area endemic for Loa loa infection. Lancet 350, 18-22 (1997).
6. M. Boussinesq, J. Gardon, J. Kamgno, S. D. Pion, N. Gardon-Wendel, J. P. Chippaux, Relationships between the prevalence and intensity of Loa loa infection in the Central province of Cameroon. Ann. Trop. Med. Parasitol. 95, 495-507 (2001).
7. J. D. Turner, N. Tendongfor, M. Esum, K. L. Johnston, R. S. Langley, L. Ford, B. Faragher, S. Specht, S. Mand, A. Hoerauf, P. Enyong, S. Wanji, M. J. Taylor, Macrofilaricidal activity after doxycycline only treatment of Onchocerca volvulus in an area of Loa loa co-endemicity: A randomized controlled trial. PLOS Negl. Trop. Dis. 4, e660 (2010).
8. P. Brouqui, P. E. Fournier, D. Raoult, Doxycycline and eradication of microfilaremia in patients with loiasis. Emerg. Infect. Dis. 7 (Suppl. 3), 604-605 (2001).
9. M. J. Taylor, W. H. Makunde, H. F. McGarry, J. D. Turner, S. Mand, A. Hoerauf, Macrofilaricidal activity after doxycycline treatment of Wuchereria bancrofti: A double-blind, randomised placebo-controlled trial. Lancet 365, 2116-2121 (2005).
10. M. Boussinesq, J. Gardon, N. Gardon-Wendel, J.-P. Chippaux, Clinical picture, epidemiology and outcome of Loa-associated serious adverse events related to mass ivermectin treatment of onchocerciasis in Cameroon. Filaria J. 2 (Suppl. 1), S4 (2003).
11. A. H. Moody, P. L. Chiodini, Methods for the detection of blood parasites. Clin. Lab. Haematol. 22, 189-201 (2000).
12. D. L. Fink, J. Kamgno, T. B. Nutman, Rapid molecular assays for specific detection and quantitation of Loa loa microfilaremia. PLOS Negl. Trop. Dis. 5, e1299 (2011).
13. B. Storey, C. Marcellino, M. Miller, M. Maclean, E. Mostafa, S. Howell, J. Sakanari, A. Wolstenholme, R. Kaplan, Utilization of computer processed high definition video imaging for measuring motility of microscopic nematode stages on a quantitative scale: "The Worminator." Int. J. Parasitol. Drugs Drug Resist. 4, 233-243 (2014).
14. N. A. Switz, M. V. D'Ambrosio, D. A. Fletcher, Low-cost mobile phone microscopy with a reversed mobile phone camera lens. PLOS One 9, e95330 (2014).
15. R. E. Miles, On the homogeneous planar Poisson point process. Math. Biosci. 6, 85-127 (1970).
16. D. N. Breslauer, R. N. Maamari, N. A. Switz, W. A. Lam, D. A. Fletcher, Mobile phone based clinical microscopy for global health applications. PLOS One 4, e6320 (2009).
17. D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, A. Ozcan, Lensfree microscopy on a cellphone. Lab Chip 10, 1787-1792 (2010).
18. I. I. Bogoch, J. R. Andrews, B. Speich, J. Utzinger, S. M. Ame, S. M. Ali, J. Keiser, Mobile phone microscopy for the diagnosis of soil-transmitted helminth infections: A proof-of-concept study. Am. J. Trop. Med. Hyg. 88, 626-629 (2013).
19. A. Skandarajah, C. D. Reber, N. A. Switz, D. A. Fletcher, Quantitative imaging with a mobile phone microscope. PLOS One 9, e96906 (2014).
20. U.S. Food and Drug Administration, Examples of MMAs the FDA has cleared or approved (2014); www.fda.gov/MedicalDevices/ProductsandMedicalProcedures/ConnectedHealth/MobileMedicalApplications/ucm368784.htm
21. G. Farnebäck, Two-frame motion estimation based on polynomial expansion, in Image Analysis, J. Bigun, T. Gustavsson, Eds. (Springer, Berlin, 2003), pp. 363-370; http://link.springer.com/chapter/10.1007/3-540-45103-X-50.