Bacterial cytoplasmic display platform Retained Display (ReD) identifies stable human germline antibody frameworks

Biotechnology Journal

Volumn 10, Issue 5, 2015, Pages 783-789

  Beasley, Matthew D ^a   Niven, Keith P ^a   Winnall, Wendy R ^b   Kiefel, Ben R ^a  

^a Affinity BIO Pty Ltd   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Bacterial display; Intrabodies; Phage display; Retained display; Single chain Fv

Indexed keywords

CELL MEMBRANES; CELLS; CHAINS; CYTOLOGY; DIAGNOSIS; ESCHERICHIA COLI; MOBILE SECURITY; PROTEINS; SCAFFOLDS (BIOLOGY);

BACTERIAL CYTOPLASM; CELLULAR MEMBRANES; EMERGING INFECTIOUS DISEASE; HIGH MOLECULAR WEIGHT; INTRABODIES; PHAGE DISPLAY; SINGLE-CHAIN FV; SURFACE DISPLAYS;

ANTIBODIES;

CYTOPLASM PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; AUTOANTIGEN; CDR1 PROTEIN, HUMAN; CDR2 PROTEIN, HUMAN; NERVE PROTEIN;

ANTIBODY ISOLATION; ANTIBODY LIBRARY; ANTIBODY SCREENING; ANTIGEN BINDING; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL PERMEABILIZATION; CYTOPLASM; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; GAMMA LIGHT CHAIN; GENE SEQUENCE; HUMAN; KAPPA LIGHT CHAIN; LIGHT CHAIN; MOLECULAR WEIGHT; MULTIGENE FAMILY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; RETAINED DISPLAY; BIOSYNTHESIS; GENETICS; METABOLISM; MOLECULAR CLONING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

AUTOANTIGENS; CLONING, MOLECULAR; CYTOPLASM; ESCHERICHIA COLI; HUMANS; NERVE TISSUE PROTEINS; SINGLE-CHAIN ANTIBODIES;

EID: 84928829297     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400560     Document Type: Article

References (24)

1. Biocca, S., Ruberti, F., Tafani, M., Pierandrei-Amaldi, P., Cattaneo, A., Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria. Biotechnology (N Y) 1995, 13, 1110-1115.
2. Martineau, P., Jones, P., Winter, G., Expression of an antibody fragment at high levels in the bacterial cytoplasm. J. Mol. Biol. 1998, 280, 117-127.
3. Auf der Maur, A., Escher, D., Barberis, A., Antigen-independent selection of stable intracellular single-chain antibodies. FEBS Lett. 2001, 508, 407-412.
4. Koch, H., Grafe, N., Schiess, R., Pluckthun, A., Direct selection of antibodies from complex libraries with the protein fragment complementation assay. J. Mol. Biol. 2006, 357, 427-441.
5. Tse, E., Lobato, M. N., Forster, A., Tanaka, T. et al., Intracellular antibody capture technology: Application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein. J. Mol. Biol. 2002, 317, 85-94.
6. Visintin, M., Tse, E., Axelson, H., Rabbitts, T. H., Cattaneo, A., Selection of antibodies for intracellular function using a two-hybrid in vivo system. Proc. Natl. Acad. Sci. U. S. A. 1999, 96, 11723-11728.
7. Jermutus, L., Honegger, A., Schwesinger, F., Hanes, J., Pluckthun, A., Tailoring in vitro evolution for protein affinity or stability. Proc. Natl. Acad. Sci. U. S. A. 2001, 98, 75-80.
8. Monsellier, E., Bedouelle, H., Improving the stability of an antibody variable fragment by a combination of knowledge-based approaches: validation and mechanisms. J. Mol. Biol. 2006, 362, 580-593.
9. Proba, K., Worn, A., Honegger, A., Pluckthun, A., Antibody scFv fragments without disulfide bonds made by molecular evolution. J. Mol. Biol. 1998, 275, 245-253.
10. Tissot, K., Ewert, S., Auf der Maur, A., Barberis, A., Escher, D., Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same. US Patent 8853362, 2002.
11. Visintin, M., Settanni, G., Maritan, A., Graziosi, S. et al., The intracellular antibody capture technology (IACT): Towards a consensus sequence for intracellular antibodies. J. Mol. Biol. 2002, 317, 73-83.
12. Labischinski, H., Goodell, E. W., Goodell, A., Hochberg, M. L., Direct proof of a "more-than-single-layered" peptidoglycan architecture of Escherichia coli W7: A neutron small-angle scattering study. J. Bacteriol. 1991, 173, 751-756.
13. Demchick, P., Koch, A. L., The permeability of the wall fabric of Escherichia coli and Bacillus subtilis. J. Bacteriol. 1996, 178, 768-773.
14. Siemering, K. R., Golbik, R., Sever, R., Haseloff, J., Mutations that suppress the thermosensitivity of green fluorescent protein. Curr. Biol. 1996, 6, 1653-1663.
15. Chen, I., Gotschlich, E. C., ComE, a competence protein from Neisseria gonorrhoeae with DNA-binding activity. J. Bacteriol. 2001, 183, 3160-3168.
16. Briers, Y., Schmelcher, M., Loessner, M. J., Hendrix, J. et al., The high-affinity peptidoglycan binding domain of Pseudomonas phage endolysin KZ144. Biochem. Biophys. Res. Commun. 2009, 383, 187-191.
17. Improta, S., Politou, A. S., Pastore, A., Immunoglobulin-like modules from titin I-band: Extensible components of muscle elasticity. Structure 1996, 4, 323-337.
18. Hust, M., Dubel, S., Human antibody gene libraries., in: Kontermann, R., Dubel, S. (Eds.), Antibody engineering, Springer-Verlag, Berlin 2010, pp. 65-84.
19. Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 2003, 278, 34167-34171.
20. Juillerat, A., Gronemeyer, T., Keppler, A., Gendreizig, S. et al., Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo. Chem. Biol. 2003, 10, 313-317.
21. Rothbauer, U., Zolghadr, K., Muyldermans, S., Schepers, A. et al., A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteins. Mol. Cell. Proteomics 2008, 7, 282-289.
22. Pluckthun, A., Mono- and bivalent antibody fragments produced in Escherichia coli: Engineering, folding and antigen binding. Immunol. Rev. 1992, 130, 151-188.
23. Stocks, M., Intrabodies as drug discovery tools and therapeutics. Curr. Opin. Chem. Biol. 2005, 9, 359-365.
24. Kortt, A. A., Dolezal, O., Power, B. E., Hudson, P. J., Dimeric and trimeric antibodies: high avidity scFvs for cancer targeting. Biomol. Eng. 2001, 18, 95-108.