Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum

BMC Biotechnology

Volumn 15, Issue 1, 2015, Pages

  Tuteja, Narendra ^a   Mishra, Panchanand ^b   Yadav, Sandep ^a   Tajrishi, Marjan ^a   Baral, Sudhir ^b   Sabat, Surendra Chandra ^b  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

^b INSTITUTE OF LIFE SCIENCES   (India)

Author keywords

CD spectroscopy; Heterologous expression; Homology modelling; Ni NTA purification; Peroxidase activity; Superoxide dismutase

Indexed keywords

CHARACTERIZATION; CIRCULAR DICHROISM SPECTROSCOPY; COPPER COMPOUNDS; ESCHERICHIA COLI; MOLECULAR OXYGEN; PLANTS (BOTANY); PURIFICATION; RECOMBINANT PROTEINS;

CD SPECTROSCOPY; HETEROLOGOUS EXPRESSION; HOMOLOGY MODELLING; PEROXIDASE ACTIVITIES; SUPER OXIDE DISMUTASE;

ENZYMES;

COMPLEMENTARY DNA; COPPER SULFATE; COPPER ZINC SUPEROXIDE DISMUTASE; PEROXIDASE; RECOMBINANT PROTEIN; ZINC SULFATE; PLANT PROTEIN; SUPEROXIDE DISMUTASE; THIOGLYCOSIDE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CHLOROPLAST; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; NONHUMAN; PEA; PLANT SEED; PROTEIN EXPRESSION; PROTEIN PURIFICATION; CHEMISTRY; CIRCULAR DICHROISM; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR MODEL; PH; TEMPERATURE;

CHLOROPLASTS; CIRCULAR DICHROISM; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; PEAS; PLANT PROTEINS; RECOMBINANT PROTEINS; SUPEROXIDE DISMUTASE; TEMPERATURE; THIOGALACTOSIDES;

EID: 84928747258     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-015-0117-0     Document Type: Article

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