Characterization of the dimer-monomer equilibrium of the papaya copper/zinc superoxide dismutase and its equilibrium shift by a single amino acid mutation

Journal of Agricultural and Food Chemistry

Volumn 47, Issue 7, 1999, Pages 2944-2949

  Lin, Chi Tsai ^a   Kuo, Ti Jung ^a   Shaw, Jei Fu ^b   Kao, Ming Ching ^c  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

^b INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

^c NATIONAL DEFENSE MEDICAL CENTER   (Taiwan)

Author keywords

Carica papaya L. cv. Tainong 2; Cu Zn SOD; Dimer monomer equilibrium shift; Papaya fruit; Superoxide dismutase

Indexed keywords

AMINO ACID; CARICA PAPAYA EXTRACT; COMPLEMENTARY DNA; COPPER ZINC SUPEROXIDE DISMUTASE; DIMER; MONOMER; PRIMER DNA; SUPEROXIDE DISMUTASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CHEMISTRY; DIMERIZATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYMOLOGY; ESCHERICHIA COLI; FRUIT; GENE EXPRESSION SYSTEM; GENETICS; METABOLISM; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; DIMERIZATION; DNA PRIMERS; FRUIT; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; SUPEROXIDE DISMUTASE;

EID: 0032877220     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf981355t     Document Type: Article

References (16)

1. Bannister, J. V.; Bannister, W. H.; Rotilio, G. Aspects of the structure, function, and application of superoxide dismutase. CRC Crit. Rev. Biochem. 1987, 22, 111-180.
2. Battistoni, A.; Folcarelli, S.; Gabbianelli, R.; Capo, C.; Rotilio, G. The Cu,Zn Superoxide dismutase from Escherichia coli retains monomeric structure at high protein concentration. Biochem. J. 1996, 320, 713-716.
3. Beauchamp, C.; Fridovich, I. Improved assays and an assay applicable to acrylamide gel. Anal. Biochem. 1971, 44, 276-287.
4. Bordo, D.; Djinovic-Carugo, K; Bolognesi, M. Conserved patterns in the Cu,Zn superoxide dismutase. J. Mol. Biol. 1994, 238, 366-368.
5. Carlsson, L. M.; Marklund, S. L.; Edlund, T. The rat extracellular superoxide dismutase dimer is converted to a tetramer by the exchange of a single amino acid. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 5219-5222.
6. Devereux, J.; Haberli, P.; Smithies, O. A comprehensive set of sequence analysis program for the VAX. Nucleic Acids Res. 1984, 12, 387-395.
7. Fridovich, I. Superoxide dismutases. Adv. Enzymol. 1986, 58, 61-97.
8. Kunkel, T. A.; Roberts, J. D.; Zakour, R. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987, 154, 367-382.
9. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
10. Lin, C. T.; Lin, M. T.; Chen, Y. T.; Shaw, J. F. Subunit interaction enhances enzyme activity and stability of sweet potato cytosolic Cu/Zn-superoxide dismutase purified by a His-tagged recombinant protein method. Plant Mol. Biol. 1995, 28, 303-311.
11. Lin, M. T.; Kuo, T. J.; Lin, C. T. Molecular cloning of a cDNA encoding copper/zinc superoxide dismutase from papaya fruit and overexpression in Escherichia coli. J. Agric. Food Chem. 1998, 46, 344-348.
12. Malinowski, D. P.; Fridovich, I. Bovine erythrocyte superoxide dismutase: diazo coupling, subunit interaction, and electrophoretic variants. Biochemistry 1979, 18, 137-144.
13. Nakano, M. Superoxide dismutase composition for periodontal use. U.S. Patent 4,842,846, 1989; pp 1-8.
14. Nakano, T.; Sato, M.; Takeuchi, M. Unique molecular properties of superoxide dismutase from teleost fish skin. FEBS Lett. 1995, 360, 197-201.
15. Rigo, A.; Terenzi, M.; Viglino, P.; Cocco, D.; Calabrese, L.; Rotilio, G. The binding of copper ions to copper-free bovine superoxide dismutase. Biochem. J. 1978, 169, 277-280.
16. Wilder, M. S.; Mass, A. Composition for preventing or alleviating skin irritation by formulations containing superoxide dismutase. U.S. Patent 4,957,740, 1990; pp 1-12.