Quantitative evaluation of bias in PCR amplification and next-generation sequencing derived from metabarcoding samples

Analytical and Bioanalytical Chemistry

Volumn 407, Issue 7, 2015, Pages 1841-1848

  Pawluczyk, Marta ^a   Weiss, Julia ^a   Links, Matthew G ^b   Egaña Aranguren, Mikel ^c,d   Wilkinson, Mark D ^c   Egea Cortines, Marcos ^a  

^a TECHNICAL UNIVERSITY OF CARTAGENA   (Spain)

^b UNIVERSITY OF SASKATCHEWAN   (Canada)

^c ETSI MontesTechnical University of Madrid   (Spain)

^d UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

Author keywords

Cq value; Ion torrent; Metabarcoding; Next generation sequencing; PCR efficiency

Indexed keywords

DNA; DNA SEQUENCES; GENE ENCODING; POINT CONTACTS;

CQ VALUE; IDENTIFICATION PROCESS; METABARCODING; NEXT-GENERATION SEQUENCING; PCR AMPLIFICATION; PCR EFFICIENCY; QUANTITATIVE EVALUATION; SPECIFIC PRIMERS;

POLYMERASE CHAIN REACTION;

GENETICS; HIGH THROUGHPUT SEQUENCING; PLANT; POLYMERASE CHAIN REACTION; PROCEDURES;

HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; PLANTS; POLYMERASE CHAIN REACTION;

EID: 84928592031     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-8435-y     Document Type: Article

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