A refined technique for extraction of extracellular matrices from bacterial biofilms and its applicability

Microbial Biotechnology

Volumn 8, Issue 3, 2015, Pages 392-403

  Chiba, Akio ^a   Sugimoto, Shinya ^a   Sato, Fumiya ^a   Hori, Seiji ^a   Mizunoe, Yoshimitsu ^a  

^a JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; DETERGENT; DNA; EXTRACELLULAR DNA; INORGANIC SALT; LITHIUM CHLORIDE; POLYSACCHARIDE; PROTEIN; SODIUM CHLORIDE; UNCLASSIFIED DRUG; BIOPOLYMER;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOFILM; CYTOTOXICITY ASSAY; ESCHERICHIA COLI; EXTRACELLULAR MATRIX; EXTRACTION; NONHUMAN; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; CHEMICAL ANALYSIS; CHEMISTRY; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGICAL EXAMINATION; PHYSIOLOGY; PROCEDURES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

BACTERIOLOGICAL TECHNIQUES; BIOFILMS; BIOPOLYMERS; CHEMISTRY TECHNIQUES, ANALYTICAL; DETERGENTS; EXTRACELLULAR MATRIX; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; SALTS; SODIUM CHLORIDE;

EID: 84928008684     PISSN: 17517907     EISSN: 17517915     Source Type: Journal    
DOI: 10.1111/1751-7915.12155     Document Type: Article

References (33)

1. Archer, N.K., Mazaitis, M.J., Costerton, J.W., Leid, J.G., Powers, M.E., and Shirtliff, M.E. (2011) Staphylococcus aureus biofilms: properties, regulation, and roles in human disease. Virulence 2: 445-459.
2. Bae, T., and Schneewind, O. (2006) Allelic replacement in Staphylococcus aureus with inducible counter-selection. Plasmid 55: 58-63.
3. Chapman, M.R., Robinson, L.S., Pinkner, J.S., Roth, R., Heuser, J., Hammar, M., etal. (2002) Role of Escherichia coli curli operons in directing amyloid fiber formation. Science 295: 851-855.
4. Costerton, J.W., Stewart, P.S., and Greenberg, E.P. (1999) Bacterial biofilms: a common cause of persistent infections. Science 284: 1318-1322.
5. Dalton, H.M., and March, P.E. (1998) Molecular genetics of bacterial attachment and biofouling. Curr Opin Biotechnol 9: 252-255.
6. Davies, D. (2003) Understanding biofilm resistance to antibacterial agents. Nat Rev Drug Discov 2: 114-122.
7. Del Pozo, J.L., and Patel, R. (2009) Clinical practice. Infection associated with prosthetic joints. N Engl J Med 361: 787-794.
8. Dunne, W.M., Jr (2002) Bacterial adhesion: seen any good biofilms lately? Clin Microbiol Rev 15: 155-166.
9. Dunne, W.M., Jr, and Burd, E.M. (1992) The effects of magnesium, calcium, EDTA, and pH on the in vitro adhesion of Staphylococcus epidermidis to plastic. Microbiol Immunol 36: 1019-1027.
10. Flemming, H.C., and Wingender, J. (2010) The biofilm matrix. Nat Rev Microbiol 8: 623-633.
11. Flemming, H.C., Neu, T.R., and Wozniak, D.J. (2007) The EPS matrix: the 'house of biofilm cells. J Bacteriol 189: 7945-7947.
12. Forsgren, A., and Sjöquist, J. (1966) 'Protein A' from S.aureus: I. Pseudo-immune reaction with human γ-globulin. J Immunol 97: 822-827.
13. Frølund, B., Palmgren, R., Keiding, K., and Nielsen, P.H. (1996) Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res 30: 1749-1758.
14. Horton, R.M., Hunt, H.D., Ho, S.N., Pullen, J.K., and Pease, L.R. (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77: 61-68.
15. Hussain, M., von Eiff, C., Sinha, B., Joost, I., Herrmann, M., Peters, G., and Becker, K. (2008) eap gene as novel target for specific identification of Staphylococcus aureus. J Clin Microbiol 46: 470-476.
16. Jucker, B.A., Harms, H., and Zehnder, A.J. (1996) Adhesion of the positively charged bacterium Stenotrophomonas (Xanthomonas) maltophilia 70401 to glass and Teflon. J Bacteriol 178: 5472-5479.
17. Kaplan, J.B., Velliyagounder, K., Ragunath, C., Rohde, H., Mack, D., Knobloch, J.K., and Ramasubbu, N. (2004) Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms. J Bacteriol 186: 8213-8220.
18. Kikuchi, T., Mizunoe, Y., Takade, A., Naito, S., and Yoshida, S. (2005) Curli fibers are required for development of biofilm architecture in Escherichia coli K-12 and enhance bacterial adherence to human uroepithelial cells. Microbiol Immunol 49: 875-884.
19. Liang, O.D., Ascencio, F., Fransson, L.A., and Wadström, T. (1992) Binding of heparan sulfate to Staphylococcus aureus. Infect Immun 60: 899-906.
20. Loferer, H., Hammar, M., and Normark, S. (1997) Availability of the fibre subunit CsgA and the nucleator protein CsgB during assembly of fibronectin-binding curli is limited by the intracellular concentration of the novel lipoprotein CsgG. Mol Microbiol 26: 11-23.
21. Maeyama, R., Mizunoe, Y., Anderson, J.M., Tanaka, M., and Matsuda, T. (2004) Confocal imaging of biofilm formation process using fluoroprobed Escherichia coli and fluoro-stained exopolysaccharide. J Biomed Mater Res A 70: 274-282.
22. Mann, E.E., Rice, K.C., Boles, B.R., Endres, J.L., Ranjit, D., Chandramohan, L., etal. (2009) Modulation of eDNA release and degradation affects Staphylococcus aureus biofilm maturation. PLoS ONE 4: e5822.
23. Mazmanian, S.K., Ton-That, H., and Schneewind, O. (2001) Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus. Mol Microbiol 40: 1049-1057.
24. O'Gara, J.P. (2007) ica and beyond: biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus. FEMS Microbiol Lett 270: 179-188.
25. O'Neill, E., Pozzi, C., Houston, P., Humphreys, H., Robinson, D.A., Loughman, A., etal. (2008) A novel Staphylococcus aureus biofilm phenotype mediated by the fibronectin-binding proteins, FnBPA and FnBPB. J Bacteriol 190: 3835-3850.
26. Palma, M., Haggar, A., and Flock, J.I. (1999) Adherence of Staphylococcus aureus is enhanced by an endogenous secreted protein with broad binding activity. J Bacteriol 181: 2840-2845.
27. Romero, D., Aguilar, C., Losick, R., and Kolter, R. (2010) Amyloid fibers provide structural integrity to Bacillus subtilis biofilms. Proc Natl Acad Sci USA 107: 2230-2234.
28. Sugimoto, S., Iwamoto, T., Takada, K., Okuda, K., Tajima, A., Iwase, T., and Mizunoe, Y. (2013) Staphylococcus epidermidis Esp degrades specific proteins associated with Staphylococcus aureus biofilm formation and host-pathogen interaction. J Bacteriol 195: 1645-1655.
29. Tabouret, M., de Rycke, J., and Dubray, G. (1992) Analysis of surface proteins of Listeria in relation to species, serovar and pathogenicity. J Gen Microbiol 138: 743-753.
30. Tapia, J.M., Muñoz, J.A., González, F., Blázquez, M.L., Malki, M., and Ballester, A. (2009) Extraction of extracellular polymeric substances from the acidophilic bacterium Acidiphilium 3.2Sup(5). Water Sci Technol 59: 1959-1967.
31. Thwaites, G.E., Edgeworth, J.D., Gkrania-Klotsas, E., Kirby, A., Tilley, R., Török, M.E., etal. (2011) Clinical management of Staphylococcus aureus bacteraemia. Lancet Infect Dis 11: 208-222.
32. Whitchurch, C.B., Tolker-Nielsen, T., Ragas, P.C., and Mattick, J.S. (2002) Extracellular DNA required for bacterial biofilm formation. Science 295: 1487.
33. Wu, J., and Xi, C. (2009) Evaluation of different methods for extracting extracellular DNA from the biofilm matrix. Appl Environ Microbiol 75: 5390-5395.