Chronic and selective inhibition of basolateral membrane Na-K-ATPase uniquely regulates brush border membrane na absorption in intestinal epithelial cells

American Journal of Physiology - Cell Physiology

Volumn 308, Issue 8, 2015, Pages C650-C656

  Manoharan, Palanikumar ^a   Gayam, Swapna ^b   Arthur, Subha ^c   Palaniappan, Balasubramanian ^c   Singh, Soudamani ^c   Dick, Gregory M ^b   Sundaram, Uma ^c  

^a UNIVERSITY OF CINCINNATI   (United States)

^b WEST VIRGINIA UNIVERSITY   (United States)

^c MARSHALL UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Intestinal Inflammation; Na glucose cotransporter; Na k atpase; Ouabain; Sglt1

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); COTRANSPORTER; GLUCOSE; OUABAIN; PHOSPHATE; SMALL INTERFERING RNA; SODIUM; SODIUM GLUCOSE COTRANSPORTER 1; SODIUM PROTON EXCHANGE PROTEIN; ENZYME INHIBITOR; SLC5A1 PROTEIN, RAT; SODIUM-HYDROGEN EXCHANGER 3;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BASOLATERAL MEMBRANE; BINDING AFFINITY; BRUSH BORDER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; GENE SILENCING; INTESTINE EPITHELIUM CELL; INTESTINE VILLUS; MAMMAL CELL; NEW ZEALAND WHITE (RABBIT); NONHUMAN; PRIORITY JOURNAL; RAT; SODIUM ABSORPTION; SODIUM CELL LEVEL; SOLUTE; UPREGULATION; ANIMAL; ANTAGONISTS AND INHIBITORS; CELL LINE; COCCIDIOSIS; CYTOLOGY; DRUG EFFECTS; EIMERIA; ENZYME ACTIVATION; ENZYMOLOGY; GENETICS; IMMUNOLOGY; INTESTINE ABSORPTION; INTESTINE MUCOSA; MALE; METABOLISM; MICROVILLUS; PARASITOLOGY; PATHOGENICITY; PATHOLOGY; PHYSIOLOGY; RABBIT; RNA INTERFERENCE; SMALL INTESTINE; TRANSPORT AT THE CELLULAR LEVEL;

MAMMALIA; ORYCTOLAGUS CUNICULUS; STROPHANTHUS GRATUS;

ANIMALS; BIOLOGICAL TRANSPORT; CELL LINE; COCCIDIOSIS; EIMERIA; ENZYME ACTIVATION; ENZYME INHIBITORS; INTESTINAL ABSORPTION; INTESTINAL MUCOSA; INTESTINE, SMALL; MALE; MICROVILLI; OUABAIN; RABBITS; RATS; RNA INTERFERENCE; RNA, SMALL INTERFERING; SODIUM; SODIUM-GLUCOSE TRANSPORTER 1; SODIUM-HYDROGEN ANTIPORTER; SODIUM-POTASSIUM-EXCHANGING ATPASE;

EID: 84928008669     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00355.2014     Document Type: Article

References (30)

1. Allgayer H, Kruis W, Paumgartner G, Wiebecke B, Brown L, Erdmann E. Inverse relationship between colonic (Na++ K+)-ATPase activity and degree of mucosal inflammation in inflammatory bowel disease. Dig Dis Sci 33: 417–422, 1988.
2. Arthur S, Coon S, Kekuda R, Sundaram U. Regulation of sodium glucose co-transporter SGLT1 through altered glycosylation in the intestinal epithelial cells. Biochim Biophys Acta 1838: 1208–1214, 2014.
3. Arthur S, Saha P, Sundaram S, Kekuda R, Sundaram U. Regulation of sodium-glutamine cotransport in villus and crypt cells by glucocorticoids during chronic enteritis. Inflamm Bowel Dis 18: 2149–2157, 2012.
4. Barrenetxe J, Sanchez O, Barber A, Gascon S, Rodriguez-Yoldi MJ, Lostao MP. TNFα regulates sugar transporters in the human intestinal epithelial cell line Caco-2. Cytokine 64: 181–187, 2013.
5. Chauhan NB, Lee JM, Siegel GJ. Na,K-ATPase mRNA levels and plaque load in Alzheimer’s disease. J Mol Neurosci 9: 151–166, 1997.
6. Coon S, Kekuda R, Saha P, Sundaram U. Glucocorticoids differentially regulate Na-bile acid cotransport in normal and chronically inflamed rabbit ileal villus cells. Am J Physiol Gastrointest Liver Physiol 298: G675– G682, 2010.
7. Coon S, Kekuda R, Saha P, Sundaram U. Reciprocal regulation of the primary sodium absorptive pathways in rat intestinal epithelial cells. Am J Physiol Cell Physiol 300: C496–C505, 2011.
8. Ejderhamn J, Finkel Y, Strandvik B. Na,K-ATPase activity in rectal mucosa of children with ulcerative colitis and Crohn’s disease. Scand J Gastroenterol 24: 1121–1125, 1989.
9. Forbush B 3rd. Assay of Na,K-ATPase in plasma membrane preparations: increasing the permeability of membrane vesicles using sodium dodecyl sulfate buffered with bovine serum albumin. Anal Biochem 128: 159–163, 1983.
10. Jordan C, Puschel B, Koob R, Drenckhahn D. Identification of a binding motif for ankyrin on the α-subunit of Na+,K+-ATPase. J Biol Chem 270: 29971–29975, 1995.
11. Kaunitz JD. Membrane transport proteins: not just for transport anymore. Am J Physiol Renal Physiol 290: F995–F996, 2006.
12. Lingrel JB, Orlowski J, Shull MM, Price EM. Molecular genetics of Na,K-ATPase. Prog Nucleic Acid Res Mol Biol 38: 37–89, 1990.
13. Oweis S, Wu L, Kiela PR, Zhao H, Malhotra D, Ghishan FK, Xie Z, Shapiro JI, Liu J. Cardiac glycoside downregulates NHE3 activity and expression in LLC-PK1 cells. Am J Physiol Renal Physiol 290: F997– F1008, 2006.
14. Pavlov KV, Sokolov VS. Electrogenic ion transport by Na+,K+-ATPase. Membr Cell Biol 13: 745–788, 2000.
15. Pressley TA, Allen JC, Clarke CH, Odebunmi T, Higham SC. Aminoterminal processing of the catalytic subunit from Na+-K+-ATPase. Am J Physiol Cell Physiol 271: C825–C832, 1996.
16. Rachmilewitz D, Karmeli F, Sharon P. Decreased colonic Na-KATPase activity in active ulcerative colitis. Isr J Med Sci 20: 681–684, 1984.
17. Rexhepaj R, Dermaku-Sopjani M, Gehring EM, Sopjani M, Kempe DS, Foller M, Lang F. Stimulation of electrogenic glucose transport by glycogen synthase kinase 3. Cell Physiol Biochem 26: 641–646, 2010.
18. Robinson JD, Flashner MS. The (Na++ K+)-activated ATPase. Enzymatic and transport properties. Biochim Biophys Acta 549: 145–176, 1979.
19. Rose AM, Valdes R Jr. Understanding the sodium pump and its relevance to disease. Clin Chem 40: 1674–1685, 1994.
20. Rubtsov AM, Lopina OD. Ankyrins. FEBS Lett 482: 1–5, 2000.
21. Saha P, Arthur S, Kekuda R, Sundaram U. Na-glutamine co-transporters B(0)AT1 in villus and SN2 in crypts are differentially altered in chronically inflamed rabbit intestine. Biochim Biophys Acta 1818: 434– 442, 2012.
22. Sundaram U, Coon S, Wisel S, West AB. Corticosteroids reverse the inhibition of Na-glucose cotransport in the chronically inflamed rabbit ileum. Am J Physiol Gastrointest Liver Physiol 276: G211–G218, 1999.
23. Sundaram U, West AB. Effect of chronic inflammation on electrolyte transport in rabbit ileal villus and crypt cells. Am J Physiol Gastrointest Liver Physiol 272: G732–G741, 1997.
24. Sundaram U, Wisel S, Coon S. Neutral Na-amino acid cotransport is differentially regulated by glucocorticoids in the normal and chronically inflamed rabbit small intestine. Am J Physiol Gastrointest Liver Physiol 292: G467–G474, 2007.
25. Sundaram U, Wisel S, Stengelin S, Kramer W, Rajendran V. Mechanism of Na+-bile cotransport during chronic ileal inflammation in rabbits. Am J Physiol Gastrointest Liver Physiol 275: G1259–G1265, 1998.
26. Sundaram U, Wisel S, Fromkes JJ. Unique mechanism of inhibition of Na+-amino acid cotransport during chronic ileal inflammation. Am J Physiol Gastrointest Liver Physiol 276: G483–G489, 1999.
27. Therien AG, Blostein R. Mechanisms of sodium pump regulation. Am J Physiol Cell Physiol 279: C541–C566, 2000.
28. Tripp JH, Muller DP, Harries JT. Mucosal (Na+-K+)-ATPase and adenylate cyclase activities in children with toddler diarrhea and the postenteritis syndrome. Pediatr Res 14: 1382–1386, 1980.
29. Wright EM, Hirayama BA, Loo DF. Active sugar transport in health and disease. J Intern Med 261: 32–43, 2007.
30. Ziegelhoffer A, Kjeldsen K, Bundgaard H, Breier A, Vrbjar N, Dzurba A. Na,K-ATPase in the myocardium: molecular principles, functional and clinical aspects. Gen Physiol Biophys 19: 9–47, 2000.