Paraffin Treasures: Do They Last Forever?

Biopreservation and Biobanking

Volumn 12, Issue 4, 2014, Pages 281-283

  Ademà, Vera ^a,b   Torres, Erica ^a   Solé, Francesc ^a,b   Serrano, Sergi ^a   Bellosillo, Beatriz ^a  

^a HOSPITAL DEL MAR   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS DNA; PARAFFIN; T LYMPHOCYTE RECEPTOR; DNA;

ARTICLE; CONTROLLED STUDY; DNA DEGRADATION; DNA EXTRACTION; GENE MUTATION; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPH NODE BIOPSY; MOLECULAR WEIGHT; MULTIPLEX POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITY CONTROL; BASE PAIRING; PROCEDURES; TIME;

BASE PAIRING; DNA; HUMANS; PARAFFIN EMBEDDING; TIME FACTORS;

EID: 84927615574     PISSN: 19475535     EISSN: 19475543     Source Type: Journal    
DOI: 10.1089/bio.2014.0010     Document Type: Article

References (7)

1. Gilbert MT, Haselkorn T, Bunce M, et al. The isolation of nucleic acids from fixed, paraffin-embedded tissues: Which methods are useful when? Plos One 2007;2:e537
2. Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 2002;161:1961-1971
3. van Dongen JJ, Langerak AW, Bruggemann M, et al. Design and standarization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report from the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia 2003;17:2257-2317
4. Hewitt SM, Lewis FA, Cao Y, et al. Tissue handling and specimen preparation in surgical pathology: Issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue. Arch Pathol Lab Med 2008;132: 1929-1935
5. Ludyga N, Grunwald B, Azimzadeh O, Englert S, Hofler H, Tapio S, Aubele M. Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses. Virchows Arch 2012;460:131-140
6. von Ahlfen S, Missel A, Bendrat K, Schlumpberger M. Determinants of RNA quality from FFPE samples. PLoS One 2007;2:e1261
7. Ribeiro-Silva A, Zhang H, Jeffrey SS. RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: A comparison of column purification and magnetic bead-based technologies. BMC Mol Biol 2007; 8:118