Selection of DNA aptamers for ovarian cancer biomarker HE4 using CE-SELEX and high-throughput sequencing

Analytical and Bioanalytical Chemistry

Volumn 407, Issue 23, 2015, Pages 6965-6973

  Eaton, Rachel M ^a   Shallcross, Jamie A ^a   Mael, Liora E ^a   Mears, Kepler S ^a   Minkoff, Lisa ^a   Scoville, Delia J ^a   Whelan, Rebecca J ^a  

^a OBERLIN COLLEGE   (United States)

Author keywords

Aptamer; Biomarker; CE SELEX; HE4; High throughput sequencing; Ovarian cancer

Indexed keywords

BINDING ENERGY; BIOINFORMATICS; CAPILLARY ELECTROPHORESIS; CLONE CELLS; DISEASES; GENE ENCODING; HELIUM; PROBES;

APTAMERS; BINDING AFFINITIES; CLONING AND SEQUENCING; COMPUTATIONAL TOOLS; FLUORESCENCE ANISOTROPY; HIGH-THROUGHPUT SEQUENCING; OVARIAN CANCERS; SEPARATION MECHANISM;

BIOMARKERS;

APTAMER; GENETIC MARKER; PROTEIN; WFDC2 PROTEIN, HUMAN;

AFFINITY CHROMATOGRAPHY; BATCH CELL CULTURE; CAPILLARY ELECTROPHORESIS; FEMALE; GENETIC MARKER; GENETICS; HIGH THROUGHPUT SEQUENCING; HUMAN; ISOLATION AND PURIFICATION; OVARIAN NEOPLASMS; PROCEDURES; SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT APTAMER TECHNIQUE;

APTAMERS, NUCLEOTIDE; BATCH CELL CULTURE TECHNIQUES; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, CAPILLARY; FEMALE; GENETIC MARKERS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; OVARIAN NEOPLASMS; PROTEINS; SELEX APTAMER TECHNIQUE;

EID: 84927534316     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-015-8665-7     Document Type: Article

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