A markerless deletion method for genetic manipulation of burkholderia cenocepacia and other multidrug-resistant gram-negative bacteria

Methods in Molecular Biology

Volumn 1197, Issue , 2014, Pages 311-327

  Aubert, Daniel F ^a   Hamad, Mohamad A ^a   Valvano, Miguel A ^a  

^a NONE

Author keywords

Burkholderia cepacia complex; Chromosomal complementation; Gene deletion; Genetics; I SceI homing endonuclease

Indexed keywords

AMPICILLIN; BACTERIAL DNA; CHLORAMPHENICOL; DNA POLYMERASE; GENOMIC DNA; GENTAMICIN; N,N DIMETHYLACETAMIDE; POLYMYXIN B; TRIMETHOPRIM; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN;

AGAR GEL ELECTROPHORESIS; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; BURKHOLDERIA CENOCEPACIA; BURKHOLDERIA CEPACIA COMPLEX; CONJUGATION; DNA BASE COMPOSITION; DNA REPLICATION ORIGIN; DNA SEQUENCE; DNA VECTOR; ESCHERICHIA COLI; GENE DELETION; GENE INSERTION; GENETIC COMPLEMENTATION; GENETIC MANIPULATION; GRAM NEGATIVE BACTERIUM; MOLECULAR CLONING; MULTIDRUG RESISTANT GRAM NEGATIVE BACTERIA; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REPORTER GENE; DRUG EFFECTS; GENETICS; METABOLISM; MULTIDRUG RESISTANCE;

BURKHOLDERIA CENOCEPACIA; BURKHOLDERIA CEPACIA COMPLEX; NEGIBACTERIA;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BURKHOLDERIA CENOCEPACIA; DRUG RESISTANCE, MULTIPLE, BACTERIAL; GRAM-NEGATIVE BACTERIA;

EID: 84927158554     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1261-2_18     Document Type: Article

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