A system for the construction of targeted unmarked gene deletions in the genus Burkholderia

Environmental Microbiology

Volumn 10, Issue 6, 2008, Pages 1652-1660

  Flannagan, Ronald S ^a   Linn, Thomas ^a   Valvano, Miguel A ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; ENDODEOXYRIBONUCLEASE SCEI; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; BURKHOLDERIA; GENE DELETION; GENE EXPRESSION REGULATION; GENE TARGETING; GENETICS; HUMAN; METABOLISM; METHODOLOGY;

BURKHOLDERIA; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, BACTERIAL; GENE DELETION; GENE TARGETING; HUMANS; MUTAGENESIS, INSERTIONAL;

ANIMALIA; BACTERIA (MICROORGANISMS); BURKHOLDERIA; BURKHOLDERIA CENOCEPACIA; NEGIBACTERIA;

EID: 43449100659     PISSN: 14622912     EISSN: 14622920     Source Type: Journal    
DOI: 10.1111/j.1462-2920.2008.01576.x     Document Type: Article

References (36)

1. Antoine, R. Locht, C. (1992) Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms. Mol Microbiol 6: 1785 1799.
2. Burt, A. Koufopanou, V. (2004) Homing endonuclease genes: the rise and fall and rise again of a selfish element. Curr Opin Genet Dev 14: 609 615.
3. Cardona, S.T. Valvano, M.A. (2005) An expression vector containing a rhamnose-inducible promoter provides tightly regulated gene expression in Burkholderia cenocepacia. Plasmid 54: 219 228.
4. Cardona, S.T., Mueller, C.L. Valvano, M.A. (2006) Identification of essential operons with a rhamnose-inducible promoter in Burkholderia cenocepacia. Appl Environ Microbiol 72: 2547 2555.
5. Choi, K.H., Gaynor, J.B., White, K.G., Lopez, C., Bosio, C.M., Karkhoff-Schweizer, R.R. Schweizer, H.P. (2005) A Tn7-based broad-range bacterial cloning and expression system. Nat Methods 2: 443 448.
6. Coenye, T. Vandamme, P. (2003) Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5: 719 729.
7. Craig, F.F., Coote, J.G., Parton, R., Freer, J.H. Gilmour, N.J. (1989) A plasmid which can be transferred between Escherichia coli and Pasteurella haemolytica by electroporation and conjugation. J Gen Microbiol 135: 2885 2890.
8. DeShazer, D. Woods, D.E. (1996) Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene. Biotechniques 20: 762 764.
9. Elzer, P.H., Kovach, M.E., Phillips, R.W., Robertson, G.T., Peterson, K.M. Roop, R.M., 2nd (1995) In vivo and in vitro stability of the broad-host-range cloning vector pBBR1MCS in six Brucella species. Plasmid 33: 51 57.
10. Figurski, D.H. Helinski, D.R. (1979) Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 76: 1648 1652.
11. Filutowicz, M. Rakowski, S.A. (1998) Regulatory implications of protein assemblies at the gamma origin of plasmid R6K - a review. Gene 223: 195 204.
12. Flannagan, R.S., Aubert, D., Kooi, C., Sokol, P.A. Valvano, M.A. (2007) Burkholderia cenocepacia requires a periplasmic HtrA protease for growth under thermal and osmotic stress and for survival in vivo. Infect Immun 75: 1679 1689.
13. Glendinning, K.J., Parsons, Y.N., Duangsonk, K., Hales, B.A., Humphreys, D., Hart, C.A. Winstanley, C. (2004) Sequence divergence in type III secretion gene clusters of the Burkholderia cepacia complex. FEMS Microbiol Lett 235: 229 235.
14. Hunt, T.A., Kooi, C., Sokol, P.A. Valvano, M.A. (2004) Identification of Burkholderia cenocepacia (formerly Burkholderia cepacia genomovar III) genes required for bacterial survival in vivo. Infect Immun 72: 4010 4022.
15. Janes, B.K. Stibitz, S. (2006) Routine markerless gene replacement in Bacillus anthracis. Infect Immun 74: 1949 1953.
16. Keith, K.E. Valvano, M.A. (2007) Characterization of SodC, a periplasmic superoxide dismutase from Burkholderia cenocepacia. Infect Immun 75: 2451 2460.
17. Lamothe, J., Huynh, K.K., Grinstein, S. Valvano, M.A. (2007) Intracellular survival of Burkholderia cenocepacia in macrophages is associated with a delay in the maturation of bacteria-containing vacuoles. Cell Microbiol 9: 40 53.
18. Lefebre, M.D. Valvano, M.A. (2002) Construction and evaluation of plasmid vectors optimized for constitutive and regulated gene expression in Burkholderia cepacia complex isolates. Appl Environ Microbiol 68: 5956 5964.
19. Lefebre, M.D., Flannagan, R.S. Valvano, M.A. (2005) A minor catalase/peroxidase from Burkholderia cenocepacia is required for normal aconitase activity. Microbiology 151: 1975 1985.
20. Lévesque, C., Brassard, S., Lapointe, J. Roy, P.H. (1994) Diversity and relative strength of tandem promoters for the antibiotic- resistance genes of several integrons. Gene 142: 49 54.
21. Loutet, S.A., Flannagan, R.S., Kooi, C., Sokol, P.A. Valvano, M.A. (2006) A complete lipopolysaccharide inner core oligosaccharide is required for resistance of Burkholderia cenocepacia to polymyxin B and bacterial survival in vivo. J Bacteriol 188: 2073 2080.
22. Mahenthiralingam, E., Coenye, T., Chung, J.W., Speert, D.P., Govan, J.R., Taylor, P. Vandamme, P. (2000) Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex. J Clin Microbiol 38: 910 913.
23. Mahenthiralingam, E., Urban, T.A. Goldberg, J.B. (2005) The multifarious, multireplicon Burkholderia cepacia complex. Nat Rev Microbiol 3: 144 156.
24. Maloney, K.E. Valvano, M.A. (2006) The mgtC gene of Burkholderia cenocepacia is required for growth under magnesium limitation conditions and intracellular survival in macrophages. Infect Immun 74: 5477 5486.
25. Marolda, C.L., Lahiry, P., Vinés, E., Saldías, S. Valvano, M.A. (2006) Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide. Methods Mol Biol 347: 237 252.
26. Miller, V.L. Mekalanos, J.J. (1988) A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 170: 2575 2583.
27. Parke, J.L. Gurian-Sherman, D. (2001) Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains. Annu Rev Phytopathol 39: 225 258.
28. Posfai, G., Kolisnychenko, V., Bereczki, Z. Blattner, F.R. (1999) Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome. Nucleic Acids Res 27: 4409 4415.
29. Prentki, P. Krisch, H.M. (1984) In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29: 303 313.
30. Sambrook, J., Fritsch, E.F. Maniatis, T. (1990) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory.
31. Sukchawalit, R., Vattanaviboon, P., Sallabhan, R. Mongkolsuk, S. (1999) Construction and characterization of regulated l-arabinose-inducible broad host range expression vectors in Xanthomonas. FEMS Microbiol Lett 181: 217 223.
32. Suzuki, N., Nonaka, H., Tsuge, Y., Okayama, S., Inui, M. Yukawa, H. (2005) Multiple large segment deletion method for Corynebacterium glutamicum. Appl Microbiol Biotechnol 69: 151 161.
33. Tomich, M., Griffith, A., Herfst, C.A., Burns, J.L. Mohr, C.D. (2003) Attenuated virulence of a Burkholderia cepacia Type III secretion mutant in a murine model of infection. Infect Immun 71: 1405 1415.
34. Valvano, M.A., Messner, P. Kosma, P. (2002) Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Microbiology 148: 1979 1989.
35. Valvano, M.A., Keith, K.E. Cardona, S.T. (2005) Survival and persistence of opportunistic Burkholderia species in host cells. Curr Op Microbiol 8: 99 105.
36. Wong, S.M. Mekalanos, J.J. (2000) Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa. Proc Natl Acad Sci USA 97: 10191 10196.