Chloroplast molecular farming: Efficient production of a thermostable xylanase by Nicotiana tabacum plants and long-term conservation of the recombinant enzyme

Protoplasma

Volumn 251, Issue 3, 2014, Pages 639-648

  Pantaleoni, Laura ^a   Longoni, Paolo ^a,d   Ferroni, Lorenzo ^b   Baldisserotto, Costanza ^b   Leelavathi, Sadhu ^c   Siva Reddy, Vanga ^c   Pancaldi, Simonetta ^b   Cella, Rino ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF FERRARA   (Italy)

^c INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

^d UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

Chloroplast transformation; Enzyme long term storage; Photosynthetic performance of tobacco transplastomic plants; Plant biofactory; Recombinant thermostable xylanase

Indexed keywords

NICOTIANA TABACUM;

RECOMBINANT PROTEIN; XYLAN 1,4 BETA XYLOSIDASE;

BIOSYNTHESIS; CHLOROPLAST; ENZYMOLOGY; GENETICS; MOLECULAR FARMING; PHOTOSYNTHESIS; TOBACCO; TRANSGENIC PLANT;

CHLOROPLASTS; MOLECULAR FARMING; PHOTOSYNTHESIS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TOBACCO; XYLOSIDASES;

EID: 84926637048     PISSN: 0033183X     EISSN: 16156102     Source Type: Journal    
DOI: 10.1007/s00709-013-0564-1     Document Type: Article

References (35)

1. Allahverdiyeva T, Aro E-M (2012) Photosynthetic responses of plants to excess light: mechanisms and conditions for photoinhibition, excess energy dissipation and repair. In: Eaton JJ, Tripathy BC, Sharkey TD (eds) Photosynthesis: plastid biology, energy conversion and carbon assimilation. Advances in photosynthesis and respiration. Springer, Dordrecht, pp 275–297
2. Anderson JM (1986) Photoregulation of the composition, function, and structure of thylakoid membranes. Annu Rev Plant Physiol Plant Mol Biol 37:93–136
3. Baldisserotto C, Ferroni L, Anfuso E, Pagnoni A, Fasulo MP, Pancaldi S (2007) Responses of Trapa natans L. floating laminae to high concentrations of manganese. Protoplasma 231:65–82
4. Baldisserotto C, Ferroni L, Zanzi C, Marchesini R, Pagnoni A, Pancaldi S (2010) Morpho-physiological and biochemical responses in the floating lamina of Trapa natans exposed to molybdenum. Protoplasma 240:83–97
5. Bally J, Nadai M, Vitel M, Rolland A, Dumain R, DubaldM(2009) Plant physiological adaptations to the massive foreign protein synthesis occurring in recombinant chloroplasts. Plant Physiol 150:1474–1481
6. Bhardwaj A, Leelavathi S, Mazumdar-Leighton S, Ghosh A, Ramakumar S, Reddy VS (2010) The critical role of N- and C-terminal contact in protein stability and folding of a family 10 xylanase under extreme conditions. Plos One 5:e11347
7. Borisjuk NV, Borjsiuk LG, Logendra S, Petersen F, Gleba Y, Raskin I (1999) Production of recombinant proteins in plant root exudates. Nat Biotechnol 17:466–469
8. Borkhardt B, Harholt J, Ulvskov P, Ahring BK, Jorgensen B, Brinch- Pedersen H (2010) Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo-xylanases. Plant Biotechnol J 8:363–374
9. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgramquantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
10. Conley AJ, Joensuu JJ, Richman A, Manassa R (2011) Protein bodyinducing fusions for high-level production and purification of recombinant proteins in plants. Plant Biotechnol J 9:419–433
11. Doran PM (2006) Foreign protein degradation and instability in plants and plant tissue cultures. Trends Biotechnol 24:426–432
12. Ghose TK (1987) Measurements of cellulase activities. Pure Appl Chem 59:257–268
13. Gupta N, Reddy VS, Maiti S, Ghosh A (2000) Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. strain NG-27. Appl Environ Microbiol 66:2631–2635
14. Hendrickson L, Furbank RT, Chow WS (2004) A simple alternative approach to assessing the fate of absorbed light energy using chlorophyll fluorescence. Photosynth Res 82:73–81
15. Horton P, Ruban AV, Walters RG (1996) Regulation of light harvesting in green plants. Annu Rev Plant Physiol Plant Mol Biol 47:655–684
16. Jahns P, Holzwarth AR (2012) The role of the xanthophyll cycle and of lutein in photoprotection of photosystem. Biochim Biophys Acta 1817:182–193
17. Juturu V, Wu JC (2012) Microbial xylanases: engineering, production and industrial applications. Biotechnol Adv 30:1219–1227
18. Kim JY, Kavas M, Fouad WM, Nong G, Preston JF, Altpeter F (2011) Production of hyperthermostable GH10 xylanase Xyl10B from Thermotoga maritima in transplastomic plants enables complete hydrolysis of methylglucuronoxylan to fermentable sugars for biofuel production. Plant Mol Biol 76:357–369
19. Klughammer C, Schreiber U (2008) Complementary PSII quantum yields calculated from simple fluorescence parameters measured byPAM fluorometry and the saturation pulse method. PAM Appl Notes 1: 27–35
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
21. Leelavathi S, Gupta N, Maiti S, Ghosh A, Reddy VS (2003) Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme. Mol Breed 11:59–67
22. Lichtenthaler HK, Buschmann C, Knapp M (2005) How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio R-Fd of leaves with the PAM fluorometer. Photosynthetica 43:379–393
23. Magee AM, Coyne S, Murphy D, Horvath EM, Medgyesy P, Kavanagh TA (2004) T7 RNA polymerase-directed expression of an antibody fragment transgene in plastids causes a semi-lethal pale-green seedling phenotype. Transgenic Res 13:325–337
24. Matsubara S, Chow WS (2004) Populations of photo inactivated photosystem II reaction centers characterized by chlorophyll a fluorescence lifetime in vivo. PNAS 101:18234–18239
25. Meyers B, Zaltsman A, Lacroix B, Kozlovsky SV, Krichevsky A (2010) Nuclear and plastid genetic engineering of plants: comparison of opportunities and challenges. Biotechnol Adv 28:747–756
26. Murashige T, Skoog F (1962) A revised mediumfor rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
27. Obembe OO, Popoola JO, Leelavathi S, Reddy SV (2011) Advances in plant molecular farming. Biotechnol Adv 29:210–222
28. Pauly M, Keegstra K (2010) Plant cell wall polymers as precursors for biofuels. Curr Opin Plant Biol 13:305–312
29. Pogorelko G, Fursova O, Lin M, Pyle E, Jass J, Zabotina OA (2011) Postsynthetic modification of plant cell walls by expression of microbial hydrolases in the apoplast. Plant Mol Biol 77:433–445
30. Ruban AV, Johnson MP, Duffy CDP (2012) The photoprotective molecular switch in the photosystem II antenna. BBA Bioenergetics 1817: 167–181
31. Wagner R, Dietzel L, Braeutigam K, Fischer W, Pfannschmidt T (2008) The long-term response to fluctuating light quality is an important and distinct light acclimation mechanism that supports survival of Arabidopsis thaliana under low light conditions. Planta 228:573–587
32. Wellburn AR (1994) The spectral determination of chlorophyll-a and chlorophyll-b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144: 307–313
33. Xu J, Dolan MC, Medrano G, Cramer CL, Weathers PJ (2012) Green factory: plants as bioproduction platforms for recombinant proteins. Biotechnol Adv 30:1171–1184
34. Zhang Z, Donaldson AA, Ma X (2012) Advancements and future directions in enzyme technology for biomass conversion. Biotechnol Adv 30:913–919
35. Zieminski K, Romanowska I, Kowalska M (2012) Enzymatic pretreatment of lignocellulosic wastes to improve biogas production. Waste Manag 32:1131–1137