Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity to accommodate foreign proteins

PLoS ONE

Volumn 10, Issue 4, 2015, Pages

  Peyret, Hadrien ^a   Gehin, Annick ^b   Thuenemann, Eva C ^a   Blond, Donatienne ^b   El Turabi, Aadil ^b,c   Beales, Lucy ^b,c   Clarke, Dean ^b   Gilbert, Robert J C ^d   Fry, Elizabeth E ^d   Stuart, David I ^d   Holmes, Kris ^b   Stonehouse, Nicola J ^b   Whelan, Mike ^c   Rosenberg, William ^c   Lomonossoff, George P ^a   Rowlands, David J ^b  

^a JOHN INNES CENTRE   (United Kingdom)

^b UNIVERSITY OF LEEDS   (United Kingdom)

^c IQUR LTD   (United Kingdom)

^d UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CORE PROTEIN; DIMER; GREEN FLUORESCENT PROTEIN; HEPATITIS B CORE ANTIGEN; POLYPEPTIDE; TANDEM CORE PROTEIN; UNCLASSIFIED DRUG; NANOBODY;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIGEN BINDING; ARTICLE; BACTERIAL EXPRESSION SYSTEM; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; COVALENT BOND; GENE EXPRESSION SYSTEM; NONHUMAN; OPEN READING FRAME; PLANT EXPRESSION SYSTEM; PROTEIN ASSEMBLY; PROTEIN STRUCTURE; VIRUS LIKE AGENT; CHEMICAL STRUCTURE; CHEMISTRY; ESCHERICHIA COLI; GENE FUSION; GENETICS; PROCEDURES; PROTEIN MULTIMERIZATION; PROTEIN QUATERNARY STRUCTURE; TOBACCO; VIRUS;

BACTERIA (MICROORGANISMS); CAMELIDAE; HEPATITIS B VIRUS;

ARTIFICIAL GENE FUSION; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HEPATITIS B CORE ANTIGENS; MODELS, MOLECULAR; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; SINGLE-DOMAIN ANTIBODIES; TOBACCO; VIRUSES;

EID: 84926629433     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0120751     Document Type: Article

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