Dual-tubed multiplex-PCR for molecular characterization of carbapenemases isolated among Acinetobacter spp. and Pseudomonas spp.

Journal of Applied Microbiology

Volumn 118, Issue 5, 2015, Pages 1096-1102

  Kazi, M ^a   Nikam, C ^a   Shetty, A ^a   Rodrigues, C ^a  

^a P D HINDUJA NATIONAL HOSPITAL   (India)

Author keywords

Carbapenem hydrolyzing class D lactamases; Metallo lactamases; Multiple carbapenemases; Multiplex PCR; New Delhi Metallo B lactamases; Non fermenters Gram negative bacilli

Indexed keywords

HYDROLYSIS;

CARBAPENEM HYDROLYZING CLASS D Β-LACTAMASE; CARBAPENEMS; CLASS-D; LACTAMASES; METALLO-Β-LACTAMASE; MULTIPLE CARBAPENEMASIS; MULTIPLEX-PCR; NEW-DELHI METALLO-B-LACTAMASE; NON FERMENTER GRAM-NEGATIVE BACILLUS; Β-LACTAMASE;

BACILLI;

AMIKACIN; AZTREONAM; BETA LACTAMASE IMP; BETA LACTAMASE KPC; BETA LACTAMASE NDM 1; BETA LACTAMASE OXA 23; BETA LACTAMASE OXA 24; BETA LACTAMASE OXA 48; BETA LACTAMASE OXA 51; BETA LACTAMASE OXA 58; BETA LACTAMASE VIM; CARBAPENEMASE; CEFEPIME; CEFTAZIDIME; CEFUROXIME; CIPROFLOXACIN; COLISTIN; EDETIC ACID; GENTAMICIN; IMIPENEM; LEVOFLOXACIN; MEROPENEM; METALLO BETA LACTAMASE; NETILMICIN; OFLOXACIN; PHENYL BORONIC ACID; PIPERACILLIN; PIPERACILLIN PLUS TAZOBACTAM; POLYMYXIN B; TOBRAMYCIN; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; BETA LACTAMASE; CARBAPENEM DERIVATIVE;

ANTIBIOTIC RESISTANCE; BACTERIUM; DETECTION METHOD; EDTA; ENZYME ACTIVITY; GENOTYPE; MOLECULAR ANALYSIS; NEW RECORD; ORGANIC ACID; POLYMERASE CHAIN REACTION; TEMPORAL ANALYSIS; TESTING METHOD;

ACINETOBACTER; ANTIMICROBIAL THERAPY; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; DUAL TUBED MULTIPLEX POLYMERASE CHAIN REACTION; ENZYME ISOLATION; GENETIC VARIABILITY; KLEBSIELLA PNEUMONIAE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PSEUDOMONAS; TURNAROUND TIME; ACINETOBACTER INFECTION; DRUG EFFECTS; ENZYMOLOGY; EVALUATION STUDY; GENETICS; HUMAN; INDIA; ISOLATION AND PURIFICATION; METABOLISM; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; PROCEDURES; PSEUDOMONAS INFECTION;

INDIA;

ACINETOBACTER; BACILLI (CLASS); KLEBSIELLA PNEUMONIAE; NEGIBACTERIA; PSEUDOMONAS;

ACINETOBACTER; ACINETOBACTER INFECTIONS; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAMASES; CARBAPENEMS; HUMANS; INDIA; KLEBSIELLA PNEUMONIAE; MICROBIAL SENSITIVITY TESTS; MULTIPLEX POLYMERASE CHAIN REACTION; PSEUDOMONAS; PSEUDOMONAS INFECTIONS;

EID: 84926528956     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12770     Document Type: Article

References (33)

1. Abbott, I., Cerqueira, G.M., Bhuiyan, S. and Peleg, A.Y. (2013) Carbapenem resistance in Acinetobacter baumannii: laboratory challenges, mechanistic insights and therapeutic strategies. Expert Rev Anti Infect Ther 11, 395-409.
2. Amudhan, S.M., Sekar, U., Arunagiri, K. and Sekar, B. (2011) OXA beta-lactamase-mediated carbapenem resistance in Acinetobacter baumannii. Indian J Med Microbiol 29, 269-274.
3. Bogaerts, P., Rezende de Castro, R., Roisin, S., Deplano, A., Huang, T.D., Hallin, M., Denis, O. and Glupczynski, Y. (2012) Emergence of NDM-1-producing Acinetobacter baumannii in Belgium. J Antimicrob Chemother 67, 1552-1553.
4. Bonomo, R.A. and Szabo, D. (2006) Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa. Clin Infect Dis 43(Suppl 2), S49-S56.
5. Chen, Z., Qlu, S., Wang, Y., Liu, S., Wang, Z., Du, X., Wang, L., Guo, J. et al. (2011) Coexistence of blaNDM-1 with the prevalent blaOXA23 and blaIMP in pan-drug resistant Acinetobacter baumannii isolates in China. Clin Infect Dis 52, 692-693.
6. Clinical and Laboratory Standards Institute (CLSI) (2012) Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement. CLSI document M100-S22.
7. El-Ageery, S.M. and Al-Hazmi, S.S. (2014) Microbiological and molecular detection of VIM-1 metallo beta lactamase-producing Acinetobacter baumannii. Eur Rev Med Pharmacol Sci 18, 965-970.
8. Feizabadi, M.M., Fathollahzadeh, B., Taherikalani, M., Rasoolinejad, M., Sadeghifard, N., Aligholi, M., Soroush, S. and Mohammadi-Yegane, S. (2008) Antimicrobial susceptibility patterns and distribution of blaOXA genes among Acinetobacter spp. Isolated from patients at Tehran hospitals. Jpn J Infect Dis 61, 274-278.
9. Galicia, M., Bermejo, K., Limansky, A., Cerón, C. and Quinones, F. (2009) Comparison of phenotypic techniques for the detection of metallo-ß-lactamases in Pseudomonas aeruginosa from respiratory isolates. Eur J Clin Microbiol Infect Dis, Abstract P2068
10. Gupta, V., Sidhu, S. and Chander, J. (2012) Metallo-β-lactamase producing nonfermentative gram-negative bacteria: an increasing clinical threat among hospitalized patients. Asian Pac J Trop Med 5, 718-721.
11. Kamolvit, W., Higgins, P.G., Paterson, D.L. and Seifert, H. (2014) Multiplex PCR to detect the genes encoding naturally occurring oxacillinases in Acinetobacter spp. J Antimicrob Chemother 69, 959-963.
12. Kaur, A., Gupta, V. and Chhina, D. (2013) Prevalence of metallo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital. Iran J Microbiol 6, 22-25.
13. Kazi, M., Drego, L., Nikam, C., Ajbani, K., Soman, R., Shetty, A. and Rodrigues, C. (2014) Molecular characterization of carbapenem-resistant Enterobacteriaceae at a tertiary care laboratory in Mumbai. Eur J Clin Microbiol Infect Dis, In Press.
14. Lee, K., Lim, Y.S., Yong, D., Yum, J.H. and Chong, Y. (2003) Evaluation of the Hodge test and the imipenem-EDTA double-disk synergy test for differentiating metallo-beta-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol 41, 4623-4629.
15. Lee, S., Park, Y.J., Kim, M., Lee, H.K., Han, K., Kang, C.S. and Kang, M.W. (2005) Prevalence of Ambler class A and D beta-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea. J Antimicrob Chemother 56, 122-127.
16. Lucena, A., Dalla Costa, L.M., Nogueira, K.S., Matos, A.P., Gales, A.C., Paganini, M.C., Castro, M.E. and Raboni, S.M. (2014) Nosocomial infections with metallo-beta-lactamase-producing Pseudomonas aeruginosa: molecular epidemiology, risk factors, clinical features and outcomes. J Hosp Infect 87, 234-240.
17. Maragakis, L.L. and Perl, T.M. (2008) Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clin Infect Dis 46, 1254-1263.
18. Molton, J.S., Tambyah, P.A., Ang, B.S., Ling, M.L. and Fisher, D.A. (2013) The global spread of healthcare-associated multidrug-resistant bacteria: a perspective from Asia. Clin Infect Dis 56, 1310-1318.
19. Mostachio, A.K., van der Heidjen, I., Rossi, F., Levin, A.S. and Costa, S.F. (2009) Multiplex PCR for rapid detection of genes encoding oxacillinases and metallo-beta-lactamases in carbapenem-resistant Acinetobacter spp. J Med Microbiol 58, 1522-1524.
20. Mugnier, P.D., Poirel, L., Naas, T. and Nordmann, P. (2010) Worldwide dissemination of the blaOXA-23 carbapenemase gene of Acinetobacter baumannii. Emerg Infect Dis 16, 35-40.
21. Niranjan, D.K., Singh, N.P., Manchanda, V., Rai, S. and Kaur, I.R. (2013) Multiple carbapenem hydrolyzing genes in clinical isolates of Acinetobacter baumannii. Indian J Med Microbiol 31, 237-241.
22. Nishio, H., Komatsu, M., Shibata, N., Shimakawa, K., Sueyoshi, N., Ura, T., Satoh, K., Toyokawa, M. et al. (2004) Metallo-beta-lactamase-producing gram-negative bacilli: laboratory-based surveillance in cooperation with 13 clinical laboratories in the Kinki region of Japan. J Clin Microbiol 42, 5256-5263.
23. Noyal, M.J., Menezes, G.A., Harish, B.N., Sujatha, S. and Parija, S.C. (2009) Simple screening tests for detection of carbapenemases in clinical isolates of nonfermentative Gram-negative bacteria. Indian J Med Res 129, 707-712.
24. Paterson, D.L. (2006) Serious infections in the intensive care unit: Pseudomonas aeruginosa and Acinetobacter baumannii. Clin Infect Dis 43, S41-S42.
25. Pitout, J.D., Revathi, G., Chow, B.L., Kabera, B., Kariuki, S., Nordmann, P. and Poirel, L. (2008) Metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from a large tertiary centre in Kenya. Clin Microbiol Infect 14, 755-759.
26. Poirel, L. and Nordmann, P. (2006) Carbapenem resistance in Acinetobacter baumannii: mechanisms and epidemiology. Clin Microbiol Infect 12, 826-836.
27. Queenan, A.M. and Bush, K. (2007) Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 20, 440-458, table of contents.
28. Rezaee, M.A., Pajand, O., Nahaei, M.R., Mahdian, R., Aghazadeh, M., Ghojazadeh, M. and Hojabri, Z. (2013) Prevalence of Ambler class A β-lactamases and ampC expression in cephalosporin-resistant isolates of Acinetobacter baumannii. Diagn Microbiol Infect Dis 76, 330-334.
29. Senkyrikova, M., Husickova, V., Chroma, M., Sauer, P., Bardon, J. and Kolar, M. (2013) Acinetobacter baumannii producing OXA-23 detected in the Czech Republic. Springerplus 2, 296.
30. Tsakris, A., Ikonomidis, A., Pournaras, S., Spanakis, N. and Markogiannakis, A. (2006) Carriage of OXA-58 but not of OXA-51 beta-lactamase gene correlates with carbapenem resistance in Acinetobacter baumannii. J Antimicrob Chemother 58, 1097-1099.
31. Van der Bij, A.K., Van der Zwan, D., Peirano, G., Severin, J.A., Pitout, J.D., Van Westreenen, M. and Goessens, W.H., Group M-PSS (2012) Metallo-ß-lactamase-producing Pseudomonas aeruginosa in the Netherlands: the nationwide emergence of a single sequence type. Clin Microbiol Infect 18, E369-E372.
32. Watanabe, A., Yanagihara, K., Matsumoto, T., Kohno, S., Aoki, N., Oguri, T., Sato, J., Muratani, T. et al. (2012) Nationwide surveillance of bacterial respiratory pathogens conducted by the Surveillance Committee of Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Clinical Microbiology in 2009: general view of the pathogens' antibacterial susceptibility. J Infect Chemother 18, 609-620.
33. Woodford, N., Ellington, M.J., Coelho, J.M., Turton, J.F., Ward, M.E., Brown, S., Amyes, S.G. and Livermore, D.M. (2006) Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents 27, 351-353.