Performance of a real-time PCR assay in routine bovine mastitis diagnostics compared with in-depth conventional culture

Journal of Dairy Research

Volumn 82, Issue 2, 2015, Pages 200-208

  Hiitiö, Heidi ^a   Riva, Rauna ^b   Autio, Tiina ^b   Pohjanvirta, Tarja ^b   Holopainen, Jani ^c   Pyörälä, Satu ^a   Pelkonen, Sinikka ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b NATL VET AND FOOD RES INSTITUTE   (Finland)

^c Thermo Fisher Scientific Oy   (Finland)

Author keywords

bacteriological culture; Bovine mastitis; diagnostics; real time PCR

Indexed keywords

BACTERIA (MICROORGANISMS); BOVINAE; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

ANIMAL; BACTERIUM; BOVINE; CLASSIFICATION; FEMALE; ISOLATION AND PURIFICATION; MASTITIS, BOVINE; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MILK; REAL TIME POLYMERASE CHAIN REACTION; VETERINARY;

ANIMALS; BACTERIA; BACTERIOLOGICAL TECHNIQUES; CATTLE; FEMALE; MASTITIS, BOVINE; MILK; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84926419104     PISSN: 00220299     EISSN: 14697629     Source Type: Journal    
DOI: 10.1017/S0022029915000084     Document Type: Article

References (31)

1. Andersen S, Dohoo IR, Olde Riekerink R & Stryhn H 2010 Diagnosing intramammary infections: evaluating expert opinions on the definition of intramammary infection using conjoint analysis. Journal of Dairy Science 93 2966-2975
2. Bexiga R, Koskinen MT, Holopainen J, Carneiro C, Pereira H, Ellis KA & Vilela CL 2011 Diagnosis of intramammary infection in samples yielding negative results or minor pathogens in conventional bacterial culturing. Journal of Dairy Research 78 49-55
3. Botaro BG, Cortinhas CS, Março LV, Moreno JFG, Silva LFP, Benites NR & Santos MV 2013 Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction. Journal of Dairy Science 96 6955-6964
4. Bradley AJ, Leach KA, Breen JE, Green LE & Green MJ 2007 Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales. Veterinary Record 160 253-258
5. Cameron M, McKenna SL, MacDonald KA, Dohoo IR, Roy JP & Keefe GP 2014 Evaluation of selective dry cow treatment following on-farm culture: risk of postcalving intramammary infection and clinical mastitis in the subsequent lactation. Journal of Dairy Science 97 270-284
6. Cederlöf SE, Toft N, Aalbaek B & Klaas IC 2012 Latent class analysis of the diagnostic characteristics of PCR and conventional bacteriological culture in diagnosing intramammary infections caused by Staphylococcus aureus in dairy cows at dry off. Acta veterinaria Scandinavica 54 65-71
7. Dohoo IR, Smith J, Andersen S, Kelton DF & Godden S 2011 Diagnosing intramammary infections: evaluation of definitions based on a single milk sample. Journal of Dairy Science 94 250-261
8. Gillespie BE & Oliver SP 2005 Simultaneous detection of mastitis pathogens, Staphylococcus aureus, Streptococcus uberis, and Streptococcus agalactiae by multiplex real-time polymerase chain reaction. Journal of Dairy Science 88 3510-3518
9. Halasa T, Huijps K, Osteras O & Hogeveen H 2007 Economic effects of bovine mastitis and mastitis management: a review. Veterinary Quarterly 29 18-31
10. Hein I, Lehner A, Rieck P, Klein K, Brandl E & Wagner M 2001 Comparison of different approaches to quantify Staphylococcus aureus cells by realtime quantitative PCR and application of this technique for examination of cheese. Applied and Environmental Microbiology 67 3122-3126
11. Hogan J, González R, Harmon R, Nickerson S, Oliver S, Pankey J & Smith K 1999 Laboratory Handbook on Bovine Mastitis. Madison, WI: National Mastitis Council
12. Honkanen-Buzalski T & Seuna E 1995 Isolation and identification of pathogens from milk. In Sandholm M, Honkanen-Buzalski T, Kaartinen L & Pyörälä S, (Eds.), The Bovine Udder and Mastitis. University of Helsinki, Faculty of Veterinary Medicine, pp. 121-141. Place of publication Gummerus, Jyväsklä.
13. Hovinen MH, Simojoki H, Pösö R, Suolaniemi J & Pyörälä S 2014 N-acetylbeta- D-glucosaminidase activity in normal bovine milk. In NMC 53rd Annual Meeting Proceedings
14. Keane OM, Budd KE, Flynn J & McCoy F 2013 Increased detection of mastitis pathogens by real-time PCR compared to bacterial culture. Veterinary Record 173 268-273
15. Koivula M, Pitkälä A, Pyörälä S & Mäntysaari E 2007 Distribution of bacteria and seasonal and regional effects in a new database for mastitis pathogens in Finland. Acta Agriculturae Scandinavica A 57 89-96
16. Koskinen MT, Wellenberg GJ, Sampimon OC, Holopainen J, Rothkamp A, Salmikivi L, van Haeringen WA, Lam TJGM & Pyörälä S 2010 Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. Journal of Dairy Science 93 5707-5715
17. Lago A, Godden SM, Bey R, Ruegg PL & Leslie K 2011 The selective treatment of clinical mastitis based on on-farm culture results: I. Effects on antibiotic use, milk withholding time, and short-term clinical and bacteriological outcomes. Journal of Dairy Science 94 4441-4456
18. Makovec JA & Ruegg PL 2003 Results of milk samples submitted for microbiological examination in Wisconsin from 1994 to 2001. Journal of Dairy Science 86 3466-3472
19. Mattila T & SandholmM1985 Antitrypsin and N-acetyl-β-d-glucosaminidase as markers of mastitis in herd of Ayrshire cows. American Journal of Veterinary Research 46 2453-2456
20. National Mastitis Council 2004 Microbiological Procedures for the Diagnosis of Bovine Udder Infection and Determination of Milk Quality, 4th edition. Madison, WI: NMC
21. Olde Riekerink RGM, Barkema HW, Kelton DF & Scholl DT 2008 Incidence rate of clinical mastitis on Canadian dairy farms. Journal of Dairy Science 91 1366-1377
22. Oliveira L, Hulland C & Ruegg PL 2013 Characterization of clinical mastitis occurring in cows on 50 large dairy herds in Wisconsin. Journal of Dairy Science 96 7538-7549
23. Paradis M-E, Haine D, Gillespie B, Oliver SP, Messier S, Comeau J & Scholl DT 2012 Bayesian estimation of the diagnostic accuracy of a multiplex real-time PCR assay and bacteriological culture for 4 common bovine intramammary pathogens. Journal of Dairy Science 95 6436-6448
24. Persson Waller K, Aspán A, Nyman A, Persson Y & Grönlund Andersson U 2011 CNS species and antimicrobial resistance in clinical and subclinical bovine mastitis. Veterinary Microbiology 152 112-116
25. Phuektes P, Mansell PD & Browning GF 2001 Multiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and streptococcal causes of bovine mastitis. Journal of Dairy Science 84 1140-1148
26. Ruegg PL 2014 Risks, realities and responsibilities associated with mastitis treatments. In NMC Regional Meeting, Ghent, Proceedings, pp. 29-35
27. Sears PM & McCarthy KK 2003 Diagnosis of mastitis for therapy decisions. The Veterinary Clinics of North America, Food animal Practice 19 93-108
28. Spittel S & Hoedemaker M 2012 Mastitis diagnosis in dairy cows using PathoProof real-time polymerase chain reaction assay in comparison with conventional bacterial culture in a Northern German field study. Berliner und Munchener tierarztliche Wochenschrift 125 494-502
29. Steeneveld W, van Werven T, Barkema HW & Hogeveen H 2014 Cowspecific treatment of clinical mastitis: an economic approach. Journal of Dairy Science 94 174-188
30. Studer E, Schaeren W, Naskova J, Pfaeffli H, Kaufmann T, Kirchhofer M, Steiner A & Graber HU 2008 A Longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Journal of Dairy Science 91 1893-1902
31. Taponen S, Salmikivi L, Simojoki H, Koskinen MT & Pyörälä S 2009 Realtime polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. Journal of Dairy Science 92 2610-2617