Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction

Journal of Dairy Science

Volumn 96, Issue 11, 2013, Pages 6955-6964

  Botaro, B G ^a   Cortinhas, C S ^a   Marco L V ^a   Moreno, J F G ^a   Silva, L F P ^a   Benites, N R ^a   Santos, M V ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Bacteria; Dairy cow; Intramammary infection

Indexed keywords

BACTERIA (MICROORGANISMS); BOS; BOVINAE; STAPHYLOCOCCUS AUREUS;

ANTIINFECTIVE AGENT; BRONOPOL; MICROCOCCAL NUCLEASE; PROPANEDIOL DERIVATIVE;

ANIMAL; BOVINE; BOVINE MASTITIS; ENZYMOLOGY; EVALUATION STUDY; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MILK; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INFECTION; VETERINARY;

ANIMALS; ANTI-INFECTIVE AGENTS; CATTLE; FEMALE; MASTITIS, BOVINE; MICROCOCCAL NUCLEASE; MILK; PROPYLENE GLYCOLS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 84886314337     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2013-6559     Document Type: Article

References (32)

1. Altman D.G. Practical Statistics for Medical Research 1991, Chapman and Hall, London, UK.
2. Barkema H.W., Schukken Y.H., Lam T.J.G.M., Galligan D.T., Beiboer M.L., Brand A. Estimation of interdependence among quarters of the bovine udder with subclinical mastitis and implications for analysis. J. Dairy Sci 1997, 80:1592-1599.
3. Barrio M.B., Rainard P., Poutrel B. Milk complement and the opsonophagocytosis and killing of Staphylococcus aureus mastitis isolates by bovine neutrophils. Microb. Pathog 2003, 34:1-9.
4. Bland J.M., Altman D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Int. J. Nurs. Stud 2010, 47:931-936.
5. Blowey R., Edmondson P. Mastitis Control in Dairy Herds 2010, CABI Publisher, London, UK. 2nd.
6. Boss R., Naskova J., Steiner A., Graber H.U. Mastitis diagnostics: Quantitative PCR for Staphylococcus aureus genotype B in bulk tank milk. J. Dairy Sci 2011, 94:128-137.
7. Bustin S.A. A-Z of Quantitative PCR 2004, International University Line, La Jolla, CA.
8. Cameotra S.S. Preservation of microorganisms as deposits for patent application. Biochem. Biophys. Res. Commun 2007, 353:849-850.
9. Cassoli L.D., Francischetti G., Machado P.F., Mourao G.B. The relationship of flow cytometry results with classical measures of bacterial counts in raw refrigerated milk. Int. J. Dairy Technol 2010, 2:297-300.
10. Fournier C., Kuhnert P., Frey J., Miserez R., Kirchhofer M., Kaufmann T., Steiner A., Graber H.U. Bovine Staphylococcus aureus: Association of virulence genes, genotypes and clinical outcome. Res. Vet. Sci 2008, 85:439-448.
11. Graber H.U., Casey M.G., Naskova J., Steiner A., Schaeren W. Development of a highly sensitive and specific assay to detect Staphylococcus aureus in bovine mastitic milk. J. Dairy Sci 2007, 90:4661-4669.
12. Graber H.U., Naskova J., Studer E., Kaufmann T., Kirchhofer M., Brechbühl M., Schaeren W., Steiner A., Fournier C. Mastitis-related subtypes of bovine Staphylococcus aureus are characterized by different clinical properties. J. Dairy Sci 2009, 92:1442-1451.
13. Hassanin A., Douzery E.J.P. The tribal radiation of the family Bovidae (Artiodactyla) and the evolution of the mitochondrial cytochrome b gene. Mol. Phylogenet. Evol 1999, 13:227-243.
14. Hein I., Jorgensen H.J., Loncarevic S., Wagner M. Quantification of Staphylococcus aureus in unpasteurised bovine and caprine milk by real-time PCR. Res. Microbiol 2005, 156:554-563.
15. Hein I., Lehner A., Rieck P., Klein K., Brandl E., Wagner M. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of cheese. Appl. Environ. Microbiol 2001, 67:3122-3126.
16. IDF (International Dairy Federation). 1995. Enumeration of somatic cells. FIL-IDF Standard no. 148A. IDF, Brussels, Belgium.
17. ISO/IDF (International Organization for Standardization/International Dairy Federation). 2006. Milk-Enumeration of somatic cells. Part 2: Guidance on the operation of fluoro-opto-electronic counters (ISO 13366-2/IDF 148-2:2006). ISO, Geneva, Switzerland.
18. Kim C.H., Khan M., Morin D.E., Hurley W.L., Tripathy D.N., Kehrli M., Oluoch A.O., Kakoma I. Optimization of the PCR for detection of Staphylococcus aureus nuc gene in bovine milk. J. Dairy Sci 2001, 84:74-83.
19. Koskinen M.T., Holopainen J., Pyörälä S., Bredbacka P., Pitkälä A., Barkema H.W., Bexiga R., Roberson J., Sølverød L., Piccinini R., Kelton D., Lehmusto H., Niskala S., Salmikivi L. Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. J. Dairy Sci 2009, 92:952-959.
20. Koskinen M.T., Wellenberg G.J., Sampimon O.C., Holopainen J., Rothkamp A., Salmikivi L., van Haeringen W.A., Lam T.J.G.M., Pyörälä S. Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. J. Dairy Sci 2010, 93:5707-5715.
21. Ludwig W., Schleifer K. How quantitative is quantitative PCR with respect to cell counts?. Syst. Appl. Microbiol 2000, 23:556-562.
22. Monardes H.G., Moore R.K., Corrigan B., Rioux Y. Preservation and storage mechanisms for raw milk samples for use in milk-recording schemes. J. Food Prot 1996, 59:151-154.
23. Office International des Épizooties. 2010. The Animal Health and Production Compendium: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Accessed Jan. 4, 2012. http://www.cabi.org/ahpc/default.aspx?site=160&page=3323.
24. Oliver S.P., Gonzalez R.N., Hogan J.S., Jayarao B.M., Owens W.N. Microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality. Natl 2004, Mastitis Counc, Verona, WI.
25. Phuektes P., Mansell P.D., Browning C.F. Multiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and streptococcal causes of bovine mastitis. J. Dairy Sci 2001, 84:1140-1148.
26. Radostits O.M., Gay C.C., Hinchcliff K.W., Constable P.D. Diseases of the mammary gland. Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pig, and Goats 2007, W. B. Saunders, Philadelphia, PA, Pages 699-702. 10th.
27. Schukken Y.H., Wilson D.J., Welcome F., Garrison-Tikofsky L., Gonzalez R.N. Monitoring udder health and milk quality using somatic cell counts. Vet. Res 2003, 34:579-596.
28. Sears P.M., Smith B.S., English P.B., Herer P.S., Gonzalez R.N. Shedding pattern of Staphylococcus aureus from bovine intramammary infections. J. Dairy Sci 1990, 73:2785-2789.
29. Sešķena R., Jankevica L. Influence of chemical preservatives on the quality and composition indices of raw milk samples. Acta Universitatis Latviensis 2007, 723:171-180.
30. Shepherd J.A., Waigh R.D., Gilbert P. Antibacterial action of 2-bromo-2-nitropropane-1,3-diol (bronopol). Antimicrob. Agents Chemother 1988, 32:1693-1698.
31. Taponen S., Salmikivi L., Simojoki H., Koskinen M.T., Pyörälä S. Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. J. Dairy Sci 2009, 92:2610-2617.
32. Wellenberg G.J., Sampimon O.C., Rothkamp A., van Haeringen W.A., Lam T.J.G.M. Detection of mastitis pathogens by real-time PCR in clinical and subclinical mastitis samples. 5th IDF Mastitis Conf 2010, Proc. Mastitis Research Into Practice, Christchurch, New Zealand, Pages 539-544.