Printing 2-Dimentional Droplet Array for Single-Cell Reverse Transcription Quantitative PCR Assay with a Microfluidic Robot

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Zhu, Ying ^a   Zhang, Yun Xia ^a   Liu, Wen Wen ^a   Ma, Yan ^a   Fang, Qun ^a   Yao, Bo ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

MICRORNA;

CELL LINE; DEVICES; EQUIPMENT DESIGN; GENETICS; HUMAN; MICROFLUIDICS; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; ROBOTICS; SINGLE CELL ANALYSIS;

CELL LINE; EQUIPMENT DESIGN; HUMANS; MICROFLUIDICS; MICRORNAS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; ROBOTICS; SINGLE-CELL ANALYSIS;

EID: 84926383372     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep09551     Document Type: Article

References (40)

1. Taniguchi, K., Kajiyama, T. & Kambara, H. Quantitative analysis of gene expression in a single cell by qPCR. Nat. Methods 6, 503-550 (2009).
2. Tang, F. C., Lao, K. Q. & Surani, M. A. Development and applications of single-cell transcriptome analysis. Nat. Methods 8S, S6-S11 (2011).
3. Lecault, V., White, A. K., Singhal, A. & Hansen, C. L. Microfluidic single cell analysis: from promise to practice. Curr. Opin. Chem. Biol. 16, 381-390 (2012).
4. Kalisky, T., Blainey, P. & Quake, S. R. Genomic analysis at the single-cell level. Annu. Rev. Genet. 45, 431-445 (2011).
5. Sanchez-Freire, V., Ebert, A. D., Kalisky, T., Quake, S. R. & Wu, J. C. Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns. Nat. Protoc. 7, 829-838 (2012).
6. Streets, A. M. et al. Microfluidic single-cell whole-transcriptome sequencing. P. Natl. Acad. Sci. U.S.A. 111, 7048-7053 (2014).
7. Guo, G. J. et al. Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev. Cell 18, 675-685 (2010).
8. Dalerba, P. et al. Single-cell dissection of transcriptional heterogeneity in human colon tumors. Nat. Biotechnol. 29, 1111-1120 (2011).
9. Marcus, J. S., Anderson, W. F. & Quake, S. R. Microfluidic single-cell mRNA isolation and analysis. Anal. Chem. 78, 3084-3089 (2006).
10. Gong, Y. A., Ogunniyi, A. O. & Love, J. C. Massively parallel detection of gene expression in single cells using subnanolitre wells. Lab Chip 10, 2334-2337 (2010).
11. Zhu, Z. et al. Single-molecule emulsion PCR in microfluidic droplets. Anal. Bioanal. Chem. 403, 2127-2143 (2012).
12. Ottesen, E. A., Hong, J. W., Quake, S. R. & Leadbetter, J. R. Microfluidic digital PCR enables multigene analysis of individual environmental bacteria. Science 314, 1464-1467 (2006).
13. Zhong, J. F. et al. A microfluidic processor for gene expression profiling of single human embryonic stem cells. Lab Chip 8, 68-74 (2008).
14. Toriello, N. M. et al. Integrated microfluidic bioprocessor for single-cell gene expression analysis. P. Natl. Acad. Sci. U.S.A. 105, 20173-20178 (2008).
15. White, A. K. et al. High-throughput microfluidic single-cell RT-qPCR. P. Natl. Acad. Sci. U.S.A. 108, 13999-14004 (2011).
16. White, A. K., Heyries, K. A., Doolin, C., VanInsberghe, M. & Hansen, C. L. Hight-hroughput microfluidic single-cell digital polymerase chain reaction. Anal. Chem. 85, 7182-7190 (2013).
17. Chiu, D. T., Lorenz, R. M. & Jeffries, G. Droplets for ultrasmall-volume analysis. Anal. Chem. 81, 5111-5118 (2009).
18. Teh, S. Y., Lin, R., Hung, L. H. & Lee, A. P. Droplet microfluidics. Lab Chip 8, 198-220 (2008).
19. Pompano, R. R., Liu, W. S., Du, W. B. & Ismagilov, R. F. Microfluidics using spatially defined arrays of droplets in one, two, and three dimensions. Annu. Rev. Anal. Chem. 4, 59-81 (2010).
20. Mohr, S. et al. Numerical and experimental study of a droplet-based PCR chip. Microfluid. Nanofluid. 3, 611-621 (2007).
21. Beer, N. R. et al. On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets. Anal. Chem. 79, 8471-8475 (2007).
22. Kiss, M. M. et al. High-throughput quantitative polymerase chain reaction in picoliter droplets. Anal. Chem. 80, 8975-8981 (2008).
23. Schaerli, Y. et al. Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Anal. Chem. 81, 302-306 (2009).
24. Eastburn, D. J., Sciambi, A. & Abate, A. R. Ultrahigh-throughput mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops. Anal. Chem. 85, 8016-8021 (2013).
25. Zhang, H. F., Jenkins, G., Zou, Y., Zhu, Z. & Yang, C. J. Massively parallel single-molecule and single-cell emulsion reverse transcription polymerase chain reaction using agarose droplet microfluidics. Anal. Chem. 84, 3599-3606 (2012).
26. Mary, P. et al. Analysis of gene expression at the single-cell level using microdroplet-based microfluidic technology. Biomicrofluidics 5, 24109 (2011).
27. Zhang, Y. X., Zhu, Y., Yao, B. & Fang, Q. Nanolitre droplet array for real time reverse transcription polymerase chain reaction. Lab Chip 11, 1545-1549 (2011).
28. Zhu, Y., Zhang, Y. X., Cai, L. & Fang, Q. Sequential operation droplet array: an automated microfluidic platform for picoliter-scale liquid handling, analysis, and screening. Anal. Chem. 85, 6723-6731 (2013).
29. Du, G. S. et al. Cell-based drug combination screening with a microfluidic droplet array system. Anal. Chem. 85, 6740-6747 (2013).
30. Zhu, Y. et al. Nanoliter-scale protein crystallization and screening with a microfluidic droplet robot. Sci. Rep. 4, 5046 (2014).
31. Chen, K. & Rajewsky, N. The evolution of gene regulation by transcription factors and microRNAs. Nat. Rev. Genet. 8, 93-103 (2007).
32. Jiang, Q. H. et al. miR2Disease: a manually curated database for microRNA deregulation in human disease. Nucleic Acids Res. 37, D98-D104 (2009).
33. Curcio, M. & Roeraade, J. Continuous segmented-flow polymerase chain reaction for high-throughput miniaturized DNA amplification. Anal. Chem. 75, 1-7 (2003).
34. Jopling, C. L. Liver-specific microRNA-122 Biogenesis and function. RNA Biol. 9, 137-142 (2012).
35. Chang, J. et al.miR-122, a mammalian liver-specific microRNA, is processed from hcr mRNA and may downregulate the high affinity cationic amino acid transporter CAT-1. RNA Biol. 1, 106-113 (2004).
36. Liu, C. S., Liu, J. J., Gao, D., Ding, M. Y. & Lin, J.M. Fabrication of microwell arrays based on two-dimensional ordered polystyrene microspheres for highthroughput single-cell analysis. Anal. Chem. 82, 9418-9424 (2010).
37. Qiao, Y. & Ma, L. Y. Predicting efficacy of cancer cell killing under hypoxic conditions with single cell DNA damage assay. Anal. Chem. 85, 6953-6957 (2013).
38. Zhang, Y. et al. Plasma microRNA-122 as a biomarker for viral-, alcohol-, and chemical-related hepatic diseases. Clin. Chem. 56, 1830-1838 (2010).
39. Gu, S. Q. et al. Multifunctional picoliter droplet manipulation platform and its application in single cell analysis. Anal. Chem. 83, 7570-7576 (2011).
40. Yu, Z. Q. et al. Nanoliter droplet array for microRNA detection based on enzymatic stem-loop probes ligation and SYBR Green real-time PCR. Talanta 85, 1760-1765 (2011).