Chemical methods for the simultaneous quantitation of metabolites and proteins from single cells

Journal of the American Chemical Society

Volumn 137, Issue 12, 2015, Pages 4066-4069

  Xue, Min ^a   Wei, Wei ^b   Su, Yapeng ^a   Kim, Jungwoo ^a   Shin, Young Shik ^b   Mai, Wilson X ^b   Nathanson, David A ^b   Heath, James R ^a,b  

^a California Institute of Technology   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMOLECULES; METABOLISM; PROTEINS;

CHEMICAL METHOD; COMPETITIVE BINDING ASSAY; CORRELATION NETWORK; EPIDERMAL GROWTH FACTOR RECEPTORS; FUNCTIONAL PROTEINS; INTRACELLULAR METABOLITES; PROTEOMIC ASSAY; QUANTITATIVE ASSAY;

METABOLITES;

CELL PROTEIN; EPIDERMAL GROWTH FACTOR RECEPTOR; ERLOTINIB; GLUCOSE; PHOSPHOPROTEIN; ANTINEOPLASTIC AGENT;

ANTIBODY COMBINING SITE; ARTICLE; COMPETITIVE BINDING ASSAY; CONTROLLED STUDY; CYTOLYSIS; ENZYME KINETICS; GLIOBLASTOMA CELL LINE; GLUCOSE TRANSPORT; GLYCOLYSIS; HUMAN; HUMAN CELL; IMMUNOASSAY; INTRACELLULAR SIGNALING; INTRACELLULAR SPACE; METABOLITE; PROTEIN PHOSPHORYLATION; QUANTITATIVE ASSAY; SINGLE CELL ANALYSIS; ANTAGONISTS AND INHIBITORS; DEVICES; DRUG SCREENING; EQUIPMENT DESIGN; FLUORESCENT ANTIBODY TECHNIQUE; GLIOBLASTOMA; METABOLISM; METABOLOMICS; MICROARRAY ANALYSIS; PROCEDURES; PROTEOMICS; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; CELL LINE, TUMOR; DRUG SCREENING ASSAYS, ANTITUMOR; EQUIPMENT DESIGN; ERLOTINIB HYDROCHLORIDE; FLUORESCENT ANTIBODY TECHNIQUE; GLIOBLASTOMA; HUMANS; METABOLOMICS; MICROARRAY ANALYSIS; PROTEOMICS; RECEPTOR, EPIDERMAL GROWTH FACTOR;

EID: 84926357740     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/jacs.5b00944     Document Type: Article

References (18)

1. Shapiro, E.; Biezuner, T.; Linnarsson, S. Nature Rev. Genet. 2013, 14, 618-630
2. Tang, F.; Lao, K.; Surani, M. A. Nat. Methods 2011, 8, S6-S11
3. Yu, J.; Zhou, J.; Sutherland, A.; Wei, W.; Shin, Y. S.; Xue, M.; Heath, J. R. Annu. Rev. Anal. Chem. 2014, 275-295
4. Zenobi, R. Science 2013, 342, 1243259
5. Ibáñez, A. J.; Fagerer, S. R.; Schmidt, A. M.; Urban, P. L.; Jefimovs, K.; Geiger, P.; Dechant, R.; Heinemann, M.; Zenobi, R. Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 8790-8794
6. Rubakhin, S. S.; Romanova, E. V.; Nemes, P.; Sweedler, J. V. Nat. Methods 2011, 8, S20-S29
7. Quake, S. R.; Scherer, A. Science 2000, 290, 1536-1540
8. Shi, Q.; Qin, L.; Wei, W.; Geng, F.; Fan, R.; Shin, Y. S.; Guo, D.; Hood, L.; Mischel, P. S.; Heath, J. R. Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 419-424
9. Bailey, R. C.; Kwong, G. A.; Radu, C. G.; Witte, O. N.; Heath, J. R. J. Am. Chem. Soc. 2007, 129, 1959-1967
10. Nathanson, D. A.; Gini, B.; Mottahedeh, J.; Visnyei, K.; Koga, T.; Gomez, G.; Eskin, A.; Hwang, K.; Wang, J.; Masui, K.; Paucar, A.; Yang, H.; Ohashi, M.; Zhu, S.; Wykosky, J.; Reed, R.; Nelson, S. F.; Cloughesy, T. F.; James, C. D.; Rao, P. N.; Kornblum, H. I.; Heath, J. R.; Cavenee, W. K.; Furnari, F. B.; Mischel, P. S. Science 2014, 343, 72-76
11. Wei, W.; Shi, Q.; Remacle, F.; Qin, L.; Shackelford, D. B.; Shin, Y. S.; Mischel, P. S.; Levine, R. D.; Heath, J. R. Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 1352-1360
12. DeBerardinis, R. J.; Thompson, C. B. Cell 2012, 148, 1132-1144
13. Matsuzawa, H.; Nirenberg, M. Proc. Natl. Acad. Sci. U. S. A. 1975, 72, 3472-3476
14. Wolf, A.; Agnihotri, S.; Micallef, J.; Mukherjee, J.; Sabha, N.; Cairns, R.; Hawkins, C.; Cuha, A. J. Exp. Med. 2011, 208, 313-326
15. Tanaka, Y.; Tran, P. O. T.; Harmon, J.; Robertson, R. P. Proc. Natl. Acad. Sci. U. S. A. 2002, 99, 12363-12368
16. Orcutt, K. P.; Parsons, A. D.; Sibenaller, Z. A.; Scarbrough, P. M.; Zhu, Y.; Sobhakumari, A.; Wilke, W. W.; Kalen, A. L.; Goswami, P.; Miller, F. J., Jr; Spitz, D. R.; Simons, A. L. Cancer Res. 2011, 71, 3932-3940
17. Riley, J. K.; Carayannopoulos, M. O.; Wyman, A. H.; Chi, M.; Moley, K. H. J. Biol. Chem. 2006, 281, 6010-6019
18. Miyamoto, S.; Murphy, A. N.; Brown, J. H. Cell Death Differ. 2008, 15, 521-529