Ca2+ and Mg2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca

Biotechnology Journal

Volumn 10, Issue 4, 2015, Pages 592-598

  Then, Johannes ^a   Wei, Ren ^a   Oeser, Thorsten ^a   Barth, Markus ^a   Belisário Ferrari, Matheus R ^a   Schmidt, Juliane ^a   Zimmermann, Wolfgang ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Calcium; Hydrolase; Polyethylene terephthalate; Polymers; Protein stability

Indexed keywords

AMINO ACIDS; CALCIUM; CIRCULAR DICHROISM SPECTROSCOPY; DICHROISM; GLASS TRANSITION; HYDROLASES; HYDROLYSIS; MOLECULAR DYNAMICS; POLYETHYLENE TEREPHTHALATES; POLYMERS; POSITIVE IONS; REACTION KINETICS; THERMODYNAMIC STABILITY;

BACTERIAL POLYESTERS; ENZYMATIC DEGRADATION; HYDROLASE; MOLECULAR DYNAMICS SIMULATIONS; POLYETHYLENE TEREPHTHALATES (PET); POLYETHYLENE-TEREPHTHALATE FILMS; PROTEIN STABILITY; REACTION TEMPERATURE;

PLASTIC BOTTLES;

ARGININE; BACTERIAL ENZYME; CALCIUM ION; ENZYME VARIANT; HYDROLASE; MAGNESIUM ION; POLYESTER; POLYETHYLENE TEREPHTHALATE; BACTERIAL PROTEIN; CALCIUM; MAGNESIUM;

ARTICLE; BINDING SITE; CATALYST; CIRCULAR DICHROISM; CONTROLLED STUDY; DEGRADATION; ENZYME DENATURATION; FILM; HYDROLYSIS; MELTING POINT; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; REACTION TIME; THERMOBIFIDA FUSCA; THERMOSTABILITY; ACTINOMYCETALES; BIOTECHNOLOGY; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; PROTEIN DENATURATION; PROTEIN ENGINEERING;

BACTERIA (MICROORGANISMS); THERMOBIFIDA FUSCA;

ACTINOMYCETALES; BACTERIAL PROTEINS; BINDING SITES; BIOTECHNOLOGY; CALCIUM; ENZYME STABILITY; HYDROLASES; MAGNESIUM; POLYETHYLENE TEREPHTHALATES; PROTEIN DENATURATION; PROTEIN ENGINEERING;

EID: 84926150760     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400620     Document Type: Article

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