Identifying nuclear protein-protein interactions using GFP affinity purification and SILAC-based quantitative mass spectrometry

Methods in Molecular Biology

Volumn 1188, Issue , 2014, Pages 207-226

  Baymaz, H Irem ^a   Spruijt, Cornelia G ^a   Vermeulen, Michiel ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

GFP affinity purification; Protein protein interactions; Quantitative mass spectrometry; SILAC

Indexed keywords

GREEN FLUORESCENT PROTEIN; NUCLEAR PROTEIN; AMINO ACID; PEPTIDE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LIQUID CHROMATOGRAPHY; MAMMAL CELL; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; RAT; TRANSGENE; AFFINITY CHROMATOGRAPHY; CELL LINE; CELL NUCLEUS; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; ISOTOPE LABELING; METABOLISM; PROCEDURES; PROTEIN DEGRADATION;

AMINO ACIDS; CELL LINE; CELL NUCLEUS; CHROMATOGRAPHY, AFFINITY; GREEN FLUORESCENT PROTEINS; ISOTOPE LABELING; MASS SPECTROMETRY; NUCLEAR PROTEINS; PEPTIDES; PROTEIN INTERACTION MAPPING; PROTEOLYSIS; TRANSFECTION;

EID: 84925883476     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1142-4_15     Document Type: Article

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