Chloroplast-expressed MSI-99 in tobacco improves disease resistance and displays inhibitory effect against rice blast fungus

International Journal of Molecular Sciences

Volumn 16, Issue 3, 2015, Pages 4628-4641

  Wang, Yun Peng ^a,b   Wei, Zheng Yi ^b   Zhang, Yu Ying ^b,c   Lin, Chun Jing ^b   Zhong, Xiao Fang ^b   Wang, Yue Lin ^b   Ma, Jing Yong ^a   Ma, Jian ^a   Xing, Shao Chen ^b  

^a JILIN AGRICULTURAL UNIVERSITY   (China)

^b JILIN ACADEMY OF AGRICULTURAL SCIENCES   (China)

^c CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Antimicrobial peptide; Chloroplast transformation; Rice blast; Tobacco

Indexed keywords

GREEN FLUORESCENT PROTEIN; MSI 99; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG; ANTIMICROBIAL CATIONIC PEPTIDE; MSI-99 PEPTIDE; PEPTIDE; RECOMBINANT PROTEIN;

ALTERNARIA ALTERNATA; ANTIFUNGAL ACTIVITY; ARTICLE; BIOLISTIC TRANSFORMATION; CHLOROPLAST; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DISEASE RESISTANCE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FUNGAL STRAIN; FUNGUS ISOLATION; MINIMUM INHIBITORY CONCENTRATION; MYCELIAL GROWTH; NONHUMAN; PLANT GROWTH; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; RICE BLAST; SOUTHERN BLOTTING; TOBACCO; WESTERN BLOTTING; ALTERNARIA; DRUG EFFECTS; GENETICS; HOST PATHOGEN INTERACTION; MAGNAPORTHE; METABOLISM; MICROBIOLOGY; ORYZA; PHYSIOLOGY; PLANT DISEASE; PLANT LEAF; PROTOPLAST; TRANSGENIC PLANT;

BACTERIA (MICROORGANISMS); MAGNAPORTHE GRISEA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

ALTERNARIA; ANTIMICROBIAL CATIONIC PEPTIDES; BLOTTING, WESTERN; CHLOROPLASTS; DISEASE RESISTANCE; ESCHERICHIA COLI; HOST-PATHOGEN INTERACTIONS; MAGNAPORTHE; MICROSCOPY, CONFOCAL; ORYZA; PEPTIDES; PLANT DISEASES; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PROTOPLASTS; RECOMBINANT PROTEINS; TOBACCO;

EID: 84925873279     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms16034628     Document Type: Article

References (33)

1. Keymanesh, K.; Soltani, S.; Sardari, S. Application of antimicrobial peptides in agriculture and food industry. World J. Microbiol. Biotechnol. 2009, 25, 933–944.
2. Kasetsomboon, T.; Kate-Ngam, S.; Sriwongchai, T.; Zhou, B.; Jantasuriyarat, C. Sequence variation of avirulence gene AVR-Pita1 in rice blast fungus, Magnaporthe oryzae. Mycol. Prog. 2013, 12, 617–628.
3. Xiang, K.; Li, W.T.; Chen, X.W.; Yuan, G.S.; Chen, W.L.; Zhang, Z.M.; Shen, Y.O.; Lin, H.J.; Pan, G.T. Clustering and expression analysis of chitinases in maize and rice. J. Life Sci. 2013, 7, 244–251.
4. Kouzai, Y.; Mochizuki, S.; Saito, A.; Ando, A.; Minami, E.; Nishizawa, Y. Expression of a bacterial chitosanase in rice plants improves disease resistance to the rice blast fungus Magnaporthe oryzae. Plant Cell Rep. 2012, 31, 629–636.
5. Zhu, X.; Chen, S.; Yang, J.; Zhou, S.; Zeng, L.; Han, J.; Wang, L.; Pan, Q. The identification of Pi50 (t), a new member of the rice blast resistance Pi2/Pi9 multigene family. Theor. Appl. Genet. 2012, 124, 1295–1304.
6. Reddy, K.V.; Yedery, R.D.; Aranha, C. Antimicrobial peptides: Premises and promises. Int. J. Antimicrob. Agents 2004, 24, 536–547.
7. Hayes, B.M.; Anderson, M.A.; Traven, A.; van der Weerden, N.L.; Bleackley, M.R. Activation of stress signalling pathways enhances tolerance of fungi to chemical fungicides and antifungal proteins. Cell. Mol. Life Sci. 2014, 71, 2651–2666.
8. Li, Q.; Lawrence, C.B.; Xing, H.Y.; Babbitt, R.A.; Bass, W.T.; Maiti, I.B.; Everett, N.P. Enhanced disease resistance conferred by expression of an antimicrobial magainin analog in transgenic tobacco. Planta 2001, 212, 635–639.
9. DeGray, G.; Rajasekaran, K.; Smith, F.; Sanford, J.; Daniell, H. Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi. Plant Physiol. 2001, 127, 852–862.
10. Chakrabarti, A.; Ganapathi, T.R.; Mukherjee, P.K.; Bapat, V.A. MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana. Planta 2003, 216, 587–596.
11. Alan, A.R.; Blowers, A.; Earle, E.D. Expression of a magainin-type antimicrobial peptide gene (MSI-99) in tomato enhances resistance to bacterial speck disease. Plant Cell Rep. 2004, 22, 388–396.
12. Hong, Y.B.; Liu, S.P.; Zhu, Y.P.; Xie, C.; Jue, D.W.; Chen, M.; Kaleri, A.H.; Yang, Q. Expression of the MSI-99m gene in transgenic potato plants confers resistance to Phytophthora infestans and Ralstonia solanacearum. Plant Mol. Biol. Rep. 2013, 31, 418–424.
13. Sang, X.; Jue, D.; Yang, L.; Bai, X.; Chen, M.; Yang, Q. Genetic transformation of Brassica napus with MSI-99m gene increases resistance in transgenic plants to Sclerotinia sclerotiorum. Mol. Plant Breed. 2013, 4, 247–253.
14. Clarke, J.L.; Daniell, H. Plastid biotechnology for crop production: Present status and future perspectives. Plant Mol. Biol. 2011, 76, 211–220.
15. Daniell, H. Production of biopharmaceuticals and vaccines in plants via the chloroplast genome. Biotechnol. J. 2006, 1, 1071–1079.
16. Grevich, J.J.; Daniell, H. Chloroplast genetic engineering: recent advances and future perspectives. Crit. Rev. Plant Sci. 2005, 24, 83–107.
17. Maliga, P. Plastid transformation in higher plants. Annu. Rev. Plant Biol. 2004, 55, 289–313.
18. Bock, R. Engineering chloroplasts for high-level foreign protein expression. In Chloroplast Biotechnology: Methods in Molecular Biology; Maliga, P., Ed.; Humana Press: New York, NY, USA, 2014; Volume 1132, pp. 93–106.
19. Day, A.; Goldschmidt-Clermont, M. The chloroplast transformation toolbox: Selectable markers and marker removal. Plant Biotechnol. J. 2011, 9, 540–553.
20. Scotti, N.; Cardi, T. Transgene-induced pleiotropic effects in transplastomic plants. Biotechnol. Lett. 2014, 36, 229–239.
21. Lee, J.Y.; Boman, A.; Sun, C.X.; Andersson, M.; Jörnvall, H.; Mutt, V.; Boman, H.G. Antibacterial peptides from pig intestine: Isolation of a mammalian cecropin. Proc. Natl. Acad. Sci. USA 1989, 86, 9159–9162.
22. Papo, N.; Shai, Y. Host defense peptides as new weapons in cancer treatment. Cell. Mol. Life Sci. 2005, 62, 784–790.
23. Hajji, M.; Jellouli, K.; Hmidet, N.; Balti, R.; Sellami-Kamoun, A.; Nasri, M. A highly thermostable antimicrobial peptide from Aspergillus clavatus ES1: Biochemical and molecular characterization. J. Ind. Microbiol. Biotechnol. 2010, 37, 805–813.
24. Seo, J.K.; Lee, M.J.; Jung, H.G.; Go, H.J.; Kim, Y.J.; Park, N.G. Antimicrobial function of SHβAP, a novel hemoglobin β chain-related antimicrobial peptide, isolated from the liver of skipjack tuna, Katsuwonus pelamis. Fish Shellfish Immun. 2014, 37, 173–183.
25. Hou, B.K.; Zhou, Y.H.; Wan, L.H.; Zhang, Z.L.; Shen, G.F.; Chen, Z.H.; Hu, Z.M. Chloroplast transformation in oilseed rape. Transgenic Res. 2003, 12, 111–114.
26. Lössl, A.G.; Waheed, M.T. Chloroplast-derived vaccines against human diseases: Achievements, challenges and scopes. Plant Biotechnol. J. 2011, 9, 527–539.
27. Oey, M.; Lohse, M.; Kreikemeyer, B.; Bock, R. Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic. Plant J. 2009, 57, 436–445.
28. Petersen, K.; Bock, R. High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome. Plant Mol. Biol. 2011, 76, 311–321.
29. Bock, R. Genetic engineering of the chloroplast: Novel tools and new applications. Curr. Opin. Biotechnol. 2014, 26, 7–13.
30. Wei, Z.Y.; Liu, Y.Z.; Lin, C.J.; Wang, Y.P.; Cai, Q.A.; Dong, Y.S.; Xing, S.C. Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop. Biotechnol. Lett. 2011, 33, 2487–2494.
31. Liu, C.W.; Lin, C.C.; Chen, J.J.; Tseng, M.J. Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment. Plant Cell Rep. 2007, 26, 1733–1744.
32. Cary, J.; Rajasekaran, K.; Jaynes, J.; Cleveland, T. Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta. Plant Sci. 2000, 154, 171–181.
33. Vasudevan, K.; Vera Cruz, C.M.; Gruissem, W; Bhullar, N.K. Large scale germplasm screening for identification of novel rice blast resistance sources. Front. Plant Sci. 2014, 5, 505.