Quantitative analysis of chemotaxis towards toluene by Pseudomonas putida in a convection-free microfluidic device

Biotechnology and Bioengineering

Volumn 112, Issue 5, 2015, Pages 896-904

  Wang, Xiaopu ^a   Atencia, Javier ^b,c   Ford, Roseanne M ^a  

^a University of Virginia   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^c UNIVERSITY OF MARYLAND   (United States)

Author keywords

Bacterial motility; Chemotactic receptor constant; Chemotactic sensitivity coefficient; Fluorescein diffusion; FM 4 64 fluorescent marker; Image analysis

Indexed keywords

BIOCHEMISTRY; BIOREMEDIATION; CHEMICAL ANALYSIS; ESCHERICHIA COLI; FLUIDIC DEVICES; FLUORESCENCE; GROUNDWATER; GROUNDWATER POLLUTION; IMAGE ANALYSIS; MICROFLUIDICS; POLLUTION; TOLUENE;

BACTERIAL MOTILITY; CHEMOTACTIC RECEPTOR CONSTANT; FLUORESCENCE INTENSITIES; FLUORESCENT MARKERS; GROUNDWATER CONTAMINATION; MICRO-FLUIDIC DEVICES; PSEUDOMONAS PUTIDA; SENSITIVITY COEFFICIENT;

BACTERIA;

GROUND WATER; TOLUENE; CHEMOTACTIC FACTOR; FLUORESCEIN; FLUORESCENT DYE; WATER POLLUTANT;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CHEMOTAXIS; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; MATHEMATICAL MODEL; MICROFLUIDICS; NONHUMAN; PSEUDOMONAS PUTIDA; QUANTITATIVE ANALYSIS; STATISTICAL PARAMETERS; THERMODYNAMICS; WATER CONTAMINATION; WATER POLLUTANT; BACTERIAL PHENOMENA AND FUNCTIONS; CYTOLOGY; DIFFUSION; EQUIPMENT DESIGN; LAB ON A CHIP; METABOLISM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS PUTIDA;

BACTERIAL PROCESSES; CHEMOTACTIC FACTORS; DIFFUSION; EQUIPMENT DESIGN; FLUORESCEIN; FLUORESCENT DYES; LAB-ON-A-CHIP DEVICES; PSEUDOMONAS PUTIDA; TOLUENE; WATER POLLUTANTS, CHEMICAL;

EID: 84925610299     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25497     Document Type: Article

References (32)

1. Ahmed T, Stocker R. 2008. Experimental verification of the behavioral foundation of bacterial transport parameters using microfluidics. Biophys J 95:4481-4493.
2. Ahmed T, Shimizu TS, Stocker R. 2010. Bacterial chemotaxis in linear and nonlinear steady microfluidic gradients. Nano Lett 10(9):3379-3385.
3. Atencia J, Cooksey GA, Locascio LE. 2012. A robust diffusion-based gradient generator for dynamic cell assays. Lab Chip 12:309-316.
4. Bhushan B, Samanta SK, Chauhan A, Chakraborti AK, Jain RK. 2000. Chemotaxis and biodegradation of 3-methyl-4-nitrophenol by ralstonia sp sj98 Biochem. Biophys Res Commun 275:129-133.
5. Chen KC, Ford RM, Cummings PT. 1998. Mathematical Models for Motile Bacterial Transport in Cylindrical Tubes. J Theor Biol 195(4):481-504.
6. Cheng SY, Heilman SM, Wasserman M, Archer S, Shuler ML, Wu M. 2007. A hydrogel-based microfluidic device for the studies of directed cell migration. Lab Chip 7:763-769.
7. Diao J, Young L, Kim S, Fogarty EA, Heilman SM, Zhou P, Shuler ML, Wu M, DeLisa MP. 2006. A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis. Lab Chip 6:381-388.
8. Ford RM, Harvey RW. 2007. Role of chemotaxis in the transport of bacteria through saturated porous media. Adv Water Resour 30:1608-1617.
9. Ford RM, Lauffenburger DA. 1991. Measurement of bacterial random motility and chemotaxis coefficients: II. Application of single-cell-based mathematical model. Biotechnol Bioeng 37:661-672.
10. Grimm AC, Harwood CS. 1997. Chemotaxis of pseudomonas spp. to the polyaromatic hydrocarbon naphthalene Appl. Environ Microbiol 63:4111-4115.
11. Hart S. 1996. In situ bioremediation: Defining the limits. Environ Sci Technol 30:398-401.
12. Harwood CS, Rivelli M, Ornston LN. 1984. Aromatic-acids are chemoattractants for pseudomonas-putida J. Bacteriol 160:622-628.
13. Harwood CS, Parales RE, Dispensa M. 1990. Chemotaxis of pseudomonas-putida toward chlorinated benzoates Appl. Environ Microbiol 56:1501-1503.
14. Hawkins AC, Harwood CS. 2002. Chemotaxis of ralstonia eutropha jmp134(pjp4) to the herbicide 2, 4-dichlorophenoxyacetate Appl. Environ Microbiol 68:968-972.
15. Kalinin YV, Jiang L, Tu Y, Wu M. 2009. Logarithmic Sensing in Escherichia coli Bacterial Chemotaxis. Biophys J 96(6):2439-2448.
16. Lanning LM, Ford RM, Long T. 2008. Bacterial chemotaxis transverse to axial flow in a microfluidic channel. Biotechnol Bioeng 100:653-663.
17. Law AMJ, Aitken MD. 2003. Bacterial chemotaxis to naphthalene desorbing from a nonaqueous liquid. Appl Environ Microb 69:5968-5973.
18. Law AMJ, Aitken MD. 2005. Continuous-flow capillary assay for measuring bacterial chemotaxis. Appl Environ Microb 71:3137-3143.
19. Lewus P, Ford RM. 2001. Quantification of random motility and chemotaxis bacterial transport coefficients using individual-cell and population-scale assays. Biotechnol Bioeng 75:292-304.
20. Liu X, Wood PL, Parales JV, Parales RE. 2009. Chemotaxis to pyrimidines and identification of a cytosine chemoreceptor in Pseudomonas putida. J Bacteriol 191:2909-2916.
21. Long T, Ford RM. 2009. Enhanced transverse migration of bacteria by chemotaxis in a porous T-sensor. Environ Sci Technol 43:1546-1552.
22. Lopez-de-Victoria G, Lovell CR. 1993. Chemotaxis of azospirillum species to aromatic-compounds Appl. Environ Microbiol 59:2951-2955.
23. Mesibov R, Ordal G, Adler J. 1973. The range of attractant concentrations for bacterial chemotaxis and the threshold and size of response over this range. J Gen Physiol 23:203.
24. Molaei M, Barry M, Stocker R, Sheng J. 2014. Failed Escape: Solid Surfaces Prevent Tumbling of Escherichia coli. Physical review letters 113(6):068103.
25. Olson MS, Ford RM, Smith JA, Fernandez EJ. 2004. Quantification of bacterial chemotaxis in porous media using magnetic resonance imaging. Environ Sci Technol 38:3864-3870.
26. Parales RE, Ditty JL, Harwood CS. 2000. Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene. Appl Environ Microbiol 66:4098-4104.
27. Rani SA, Pitts B, Stewart PS. 2005. Rapid diffusion of fluorescent tracers into staphylococcus epidermidis biofilms visualized by time lapse microscopy. Antimicrob Agents Chemother 49(2):728-732.
28. Roper MG, Shackman JG, Dahlgren GM, Kennedy RT. 2003. Microfluidic chip for continuous monitoring of hormone secretion from live cells using an electrophoresis-based immunoassay. Anal Chem 75:4711-4717.
29. Samanta SK, Bhushan B, Chauhan A, Jain RK. 2000. Chemotaxis of a ralstonia sp sj98 toward different nitroaromatic compounds and their degradation. Biochem Biophys Res Commun 269:117-123.
30. Scow KM, Hicks KA. 2005. Natural attenuation and enhanced bioremediation of organic contaminants in groundwater. Curr Opin Biotechnol 16(3):246-253.
31. Wang X, Long T, Ford RM. 2012. Bacterial chemotaxis toward a NAPL source within a pore-scale microfluidic chamber. Biotechnol Bioeng 109(7):1622-1628.
32. Wolfe AJ, Conley MP, Kramer TJ, Berg HC. 1987. Reconstitution of signaling in bacterial chemotaxis. J Bacteriol 169:1878-1885.