A single-vector EYFP reporter gene assay for G protein-coupled receptors

Analytical Biochemistry

Volumn 476, Issue , 2015, Pages 40-44

  Hald, Helle ^a   Wu, Boqian ^b   Bouakaz, Lamine ^c   Meldal, Morten ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b CARLSBERG LABORATORY   (Denmark)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Cell Image Analyzer software; EYFP; G protein coupled receptors; GPCR assay cells; Monoclonal; Reporter gene assay

Indexed keywords

CELL CULTURE; CLONE CELLS; GENES; MOLECULAR BIOLOGY; PEPTIDES; SIGNAL TRANSDUCTION;

CELL IMAGES; EYFP; G PROTEIN COUPLED RECEPTORS; MONOCLONAL; REPORTER GENE ASSAYS;

SIGNAL TO NOISE RATIO;

CYCLIC AMP; G PROTEIN COUPLED RECEPTOR; HYPOPHYSIS ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE RECEPTOR; MELANOCORTIN 4 RECEPTOR; TRICHOSTATIN A; VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1; VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2; YELLOW FLUORESCENT PROTEIN; BACTERIAL PROTEIN; PHOTOPROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE EXPRESSION ASSAY; GENETIC TRANSFECTION; HEK293 CELL LINE; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; SENSITIVITY ANALYSIS; SIGNAL NOISE RATIO; SIGNAL TRANSDUCTION; YELLOW FLUORESCENT PROTEIN REPORTER GENE ASSAY; COMPUTER PROGRAM; GENETICS; METABOLISM;

BACTERIAL PROTEINS; GENES, REPORTER; HEK293 CELLS; HUMANS; LUMINESCENT PROTEINS; RECEPTORS, G-PROTEIN-COUPLED; SOFTWARE;

EID: 84925597905     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.02.003     Document Type: Article

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