High-yield production of manganese peroxidase, lignin peroxidase, and versatile peroxidase in Phanerochaete chrysosporium

Applied Microbiology and Biotechnology

Volumn 98, Issue 22, 2014, Pages 9283-9294

  Coconi Linares, Nancy ^a   Magaña Ortíz, Denis ^a   Guzmán Ortiz, Doralinda A ^a   Fernández, Francisco ^b   Loske, Achim M ^b   Gómez Lim, Miguel A ^a  

^a DEPARTAMENTO DE INGENIERÍA GENÉTICA   (Mexico)

^b CENTRO DE FÍSICA APLICADA Y TECNOLOGÍA AVANZADA   (Mexico)

Author keywords

Fungal transformation; Heterologous expression; Phanerochaete chrysosporium; Shock waves; Versatile peroxidase; White rot fungi

Indexed keywords

BIOTECHNOLOGY; ENZYME ACTIVITY; GENE EXPRESSION; LIGNIN; MANGANESE; METABOLISM; POLYMERASE CHAIN REACTION; SHOCK WAVES;

FUNGAL TRANSFORMATION; HETEROLOGOUS EXPRESSION; PHANEROCHAETE CHRYSOSPORIUM; VERSATILE PEROXIDASE; WHITE ROT FUNGI;

POLYSULFONES;

FUNGAL ENZYME; LIGNIN PEROXIDASE; MANGANESE PEROXIDASE; PEROXIDASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VERSATILE PEROXIDASE;

ARTICLE; CONTROLLED STUDY; DNA MODIFICATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; FUNGAL BIOMASS; GENE EXPRESSION; GENETIC MANIPULATION; NONHUMAN; PHANEROCHAETE; PLEUROTUS ERYNGII; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RECOMBINANT GENE; SOUTHERN BLOTTING; DNA SEQUENCE; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PLEUROTUS;

CLONING, MOLECULAR; GENE EXPRESSION; METABOLIC ENGINEERING; MOLECULAR SEQUENCE DATA; PEROXIDASES; PHANEROCHAETE; PLEUROTUS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC;

EID: 84925489180     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-6105-9     Document Type: Article

References (47)

1. Abdel-Hamid AM, Solbiati JO, Cann IK (2012) Insights into lignin degradation and its potential industrial applications. Adv Appl Microbiol 82:1–28
2. Alic M, Letzring C, Gold MH (1987) Mating system and basidiospore formation in the lignin-degrading basidiomycete Phanerochaete chrysosporium. Appl Environ Microbiol 53:1464–1469
3. 2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli. Biotechnol Lett 34:1537–1543
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
5. Camarero S, Sarkar S, Ruiz-Dueñas FJ, Martínez MJ, Martínez ÁT (1999) Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites. J Biol Chem 274:10324–10330
6. Conesa A, van den Hondel CA, Punt PJ (2000) Studies on the production of fungal peroxidases in Aspergillus niger. Appl Environ Microbiol 66:3016–3023
7. Dashtban M, Schraft H, Syed TA, Qin W (2010) Fungal biodegradation and enzymatic modification of lignin. Int J Biochem Mol Biol 1:36–50
8. Davila-Vazquez G, Tinoco R, Pickard MA, Vazquez-Duhalt R (2005) Transformation of halogenated pesticides by versatile peroxidase from Bjerkandera adusta. Enzym Microb Technol 36:223–231
9. Eibes GM, Lu-Chau TA, Ruiz-Dueñas FJ, Feijoo G, Martínez MJ, Martínez AT, Lema JM (2009) Effect of culture temperature on the heterologous expression of Pleurotus eryngii versatile peroxidase in Aspergillus hosts. Bioprocess Biosyst Eng 32:129–134
10. Elisashvili V, Kachlishvili E (2009) Physiological regulation of laccase and manganese peroxidase production by white-rot Basidiomycetes. J Biotechnol 144:37–42
11. Fischer CR, Klein-Marcuschamer D, Stephanopoulos G (2008) Selection and optimization of microbial hosts for biofuels production. Metab Eng 10:295–304
12. Garcia-Ruiz E, Gonzalez-Perez D, Ruiz-Dueñas FJ, Martínez AT, Alcalde M (2012) Directed evolution of a temperature-, peroxide-and alkaline pH-tolerant versatile peroxidase. Biochem J 441:487–498
13. Gettemy JM, Ma B, Alic M, Gold MH (1998) Reverse transcription-PCR analysis of the regulation of the manganese peroxidase gene family. Appl Environ Microbiol 64:569–574
14. Harmsen MC, Schuren FHJ, Moukha SM, van Zuilen CM, Punt PJ, Wessels JGH (1992) Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetes Schizophyllum commune, Phanerochaete chrysosporium and Agaricus bisporus. Curr Genet 22:447–454
15. Heinfling A, Ruiz-Dueñas FJ, Martínez MJ, Bergbauer M, Szewzyk U, Martínez AT (1998) A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta. FEBS Lett 428:141–146
16. Irie T, Honda Y, Watanabe T, Kuwahara M (2001) Homologous expression of recombinant manganese peroxidase genes in ligninolytic fungus Pleurotus ostreatus. Appl Microbiol Biotechnol 55:566–570
17. Janusz G, Kucharzyk KH, Pawlik A, Staszczak M, Paszczynski AJ (2013) Fungal laccase, manganese peroxidase and lignin peroxidase: gene expression and regulation. Enzym Microb Technol 52:1–12
18. Jiang F, Kongsaeree P, Charron R, Lajoie C, Xu H, Scott G, Kelly C (2008) Production and separation of manganese peroxidase from heme amended yeast cultures. Biotechnol Bioeng 99:540–549
19. Johnson TM, Li JKK (1991) Heterologous expression and characterization of an active lignin peroxidase from Phanerochaete chrysosporium using recombinant baculovirus. Arch Biochem Biophys 291:371–378
20. Kaminskyj SGW (2001) Fundamentals of growth, storage, genetics and microscopy of Aspergillus nidulans. Fungal Genet Newsl 48:25–31
21. Kamitsuji H, Watanabe T, Honda Y, Kuwahara M (2005) Direct oxidation of polymeric substrates by multifunctional manganese peroxidase isoenzyme from Pleurotus ostreatus without redox mediators. Biochem J 386:387–93
22. Knežević A, Milovanović I, Stajić M, Lončar N, Brčeski I, Vukojević J, Cilerdžić J (2013) Lignin degradation by selected fungal species. Bioresour Technol 138:117–123
23. 2+-deficiency reveals a key role for the Pleurotus ostreatus versatile peroxidase (VP4) in oxidation of aromatic compounds. Appl Microbiol Biotechnol 98:6795–6804
24. Laemmli UK (1970) Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature 227:680–685
25. Ma B, Mayfield MB, Gold MH (2003) Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker. Curr Genet 43:407–414
26. Magaña-Ortíz D, Coconi-Linares N, Ortiz-Vazquez E, Fernández F, Loske AM, Gómez-Lim MA (2013) A novel and highly efficient method for genetic transformation of fungi employing shock waves. Fungal Genet Biol 56:9–16
27. Martínez MJ, Ruiz-Dueñas FJ, Guillén F, Martínez ÁT (1996) Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii. Eur J Biochem 237:424–432
28. Martínez AT, Speranza M, Ruiz-Dueñas FJ, Ferreira P, Camarero S, Guillén F, Martínez MJ, Gutiérrez A, del Río JC (2005) Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin. Int Microbiol 8:195–204
29. Mayfield MB, Kishi K, Alic M, Gold MH (1994) Homologous expression of recombinant manganese peroxidase in Phanerochaete chrysosporium. Appl Environ Microbiol 60:4303–4309
30. Meyer V (2008) Genetic engineering of filamentous fungi—progress, obstacles and future trends. Biotechnol Adv 26:177–185
31. Moreira PR, Almeida-Vara E, Sena-Martins G, Polonia I, Malcata FX, Duarte JC (2001) Decolourisation of Remazol Brilliant Blue R via a novel Bjerkandera sp. strain. J Biotechnol 89:107–111
32. Moreira PR, Almeida-Vara E, Malcata FX, Duarte JC (2007) Lignin transformation by a versatile peroxidase from a novel Bjerkandera sp. strain. Int Biodeter Biodegr 59:234–238
33. Nicolaou SA, Gaida SM, Papoutsakis ET (2010) A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: from biofuels and chemicals, to biocatalysis and bioremediation. Metab Eng 12:307–331
34. Palma C, Martínez AT, Lema JM, Martínez MJ (2000) Different fungal manganese-oxidizing peroxidases: a comparison between Bjerkandera sp. and Phanerochaete chrysosporium. J Biotechnol 77:235–245
35. Pérez-Boada M, Doyle WA, Ruiz-Dueñas FJ, Martínez MJ, Martínez AT, Smith AT (2002) Expression of Pleurotus eryngii versatile peroxidase in Escherichia coli and optimisation of in vitro folding. Enzym Microb Technol 30:518–524
36. Punekar NS, Suresh-Kumar SV, Jayashri TN (2003) Isolation of genomic DNA from acetone-dried Aspergillus mycelia. Fungal Genet Newsl 50:15–16
37. 2+ and different aromatic substrates. Appl Environ Microbiol 65:4705–4707
38. Ruiz-Dueñas FJ, Morales M, Garcia E, Miki Y, Martínez MJ, Martínez AT (2009) Substrate oxidation sites in versatile peroxidase and other basidiomycete peroxidases. J Exp Bot 60:441–452
39. Salame TM, Knop D, Levinson D, Mabjeesh SJ, Yarden O, Hadar Y (2014) Inactivation of a Pleurotus ostreatus versatile peroxidase-encoding gene (mnp2) results in reduced lignin degradation. Environ Microbiol 16:265–77
40. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Harbor Press, Cold Spring Harbor, NY
41. Singh D, Chen S (2008) The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes. Appl Microbiol Biotechnol 81:399–417
42. Sollewijn Gelpke MD, Mayfield-Gambill M, Lin Cereghino GP, Gold MH (1999) Homologous expression of recombinant lignin peroxidase in Phanerochaete chrysosporium. Appl Environ Microbiol 65:1670–1674
43. Stewart P, Kersten P, Vanden Wymelenberg A, Gaskell J, Cullen D (1992) Lignin peroxidase gene family of Phanerochaete chrysosporium: complex regulation by carbon and nitrogen limitation and identification of a second dimorphic chromosome. J Bacteriol 174:5036–5042
44. Tien M, Kirk T (1988) Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol 161:238–249
45. Tokuoka M, Tanaka M, Ono K, Takagi S, Shintani T, Gomi K (2008) Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression. Appl Environ Microbiol 74:6538–6546
46. Tsukihara T, Honda Y, Sakai R, Watanabe T, Watanabe T (2006) Exclusive overproduction of recombinant versatile peroxidase MnP2 by genetically modified white rot fungus, Pleurotus ostreatus. J Biotechnol 126:431–439
47. Wariishi H, Valli K, Gold MH (1992) Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators. J Biol Chem 267:23688–23695