Expression of Pleurotus eryngii versatile peroxidase in Escherichia coli and optimisation of in vitro folding

Enzyme and Microbial Technology

Volumn 30, Issue 4, 2002, Pages 518-524

  Perez Boada M ^a   Doyle, W A ^b   Ruiz Duenas F J ^a   Martinez M J ^a   Martinez A T ^a   Smith, A T ^b  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b UNIVERSITY OF SUSSEX   (United Kingdom)

Author keywords

Escherichia coli; Heterologous expression; In vitro folding; Ligninolytic peroxidase; Mn2+ oxidation; Pleurotus eryngii; Recombinant peroxidase; Versatile peroxidase

Indexed keywords

CLONING; CONCENTRATION (PROCESS); DNA; ENZYMES; FUNGI; OPTIMIZATION; PH EFFECTS; POLYPEPTIDES;

HETEROLOGOUS EXPRESSION;

ESCHERICHIA COLI;

CALCIUM ION; FUNGAL ENZYME; GLUTATHIONE; GLUTATHIONE DISULFIDE; HEMIN; LIGNIN PEROXIDASE; MANGANESE; PEROXIDASE; POLYPEPTIDE; PYRANOSIDE; RECOMBINANT PROTEIN; UREA;

ALLELE; ARTICLE; CELL INCLUSION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME INDUCTION; ENZYME KINETICS; ESCHERICHIA COLI; EXPRESSION VECTOR; MOLECULAR CLONING; NONHUMAN; PH; PHANEROCHAETE; PLEUROTUS; PLEUROTUS ERYNGII; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN FOLDING;

CHRYSOSPORIUM; ESCHERICHIA COLI; PHANEROCHAETE; PHANEROCHAETE CHRYSOSPORIUM; PLEUROTUS; PLEUROTUS ERYNGII; VECTORS;

EID: 0037117794     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00008-X     Document Type: Article

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