Simultaneous detection and differentiation of highly virulent and classical Chinese-type isolation of PRRSV by real-time RT-PCR

Journal of Immunology Research

Volumn 2014, Issue , 2014, Pages

  Xiao, Shuqi ^a   Chen, Yaosheng ^a   Wang, Liangliang ^a   Gao, Jintao ^a   Mo, Delin ^a   He, Zuyong ^a   Liu, Xiaohong ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR GEL ELECTROPHORESIS; ARTICLE; BLOOD SAMPLING; CHINA; CONTROLLED STUDY; HERD; HOST CELL; NONHUMAN; PIG; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS GENOME; VIRUS ISOLATION; VIRUS VIRULENCE; ANIMAL; CLASSIFICATION; GENETICS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VIROLOGY;

VIRUS PROTEIN;

ANIMALS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SWINE; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84925346513     PISSN: 23148861     EISSN: 23147156     Source Type: Journal    
DOI: 10.1155/2014/809656     Document Type: Article

References (16)

1. S. Xiao, J. Jia, D. Mo, et al., "Understanding PRRSV infection in porcine lung based on genome-wide transcriptome response identified by deep sequencing, " PLoS ONE, vol. 5, no. 6, Article ID e11377, 2010.
2. K. Tian, X. Yu, T. Zhao, et al., "Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark, " PLoS ONE, vol. 2, no. 6, article e526, 2007.
3. Y. Li, X. Wang, K. Bo, et al., "Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China, " Veterinary Journal, vol. 174, no. 3, pp. 577-584, 2007.
4. G.-Z. Tong, Y.-J. Zhou, X.-F. Hao, Z.-J. Tian, T.-Q. An, and H.-J. Qiu, "Highly pathogenic porcine reproductive and respiratory syndrome, China, " Emerging Infectious Diseases, vol. 13, no. 9, pp. 1434-1436, 2007.
5. Y. Feng, T. Zhao, T. Nguyen, et al., "Porcine respiratory and reproductive syndrome virus variants, Vietnam and China, 2007, " Emerging Infectious Diseases, vol. 14, no. 11, pp. 1774-1776, 2008.
6. M. Ying, Y. Feng, D. Liu, and G. F. Gao, "Avian influenza virus, streptococcus suis serotype 2, severe acute respiratory syndrome-coronavirus and beyond: molecular epidemiology, ecology and the situation in China, " Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 364, no. 1530, pp. 2725-2737, 2009.
7. D. Normile, " China, Vietnam grapple with âAIJrapidly evolvingâAI pig virus, " Science, vol. 317, no. 5841, p. 1017, 2007.
8. Y.-J. Zhou, X.-F. Hao, Z.-J. Tian, et al., "Highly virulent porcine reproductive and respiratory syndrome virus emerged in China, " Transboundary and Emerging Diseases, vol. 55, no. 3-4, pp. 152-164, 2008.
9. W. Lurchachaiwong, S. Payungporn, U. Srisatidnarakul, C. Mungkundar, A. Theamboonlers, and Y. Poovorawan, "Rapid detection and strain identification of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR, " Letters in Applied Microbiology, vol. 46, no. 1, pp. 55-60, 2008.
10. E. Martnez, P. Riera, M. Sitjə, Y. Fang, S. Oliveira, and J. Maldonado, "Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green, " Research in Veterinary Science, vol. 85, no. 1, pp. 184-193, 2008.
11. A. Wasilk, J. D. Callahan, J. Christopher-Hennings, et al., "Detection of U. S., lelystad, and european-like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR, " Journal of Clinical Microbiology, vol. 42, no. 10, pp. 4453-4461, 2004.
12. X.-L. Xiao, H. Wu, Y.-G. Yu, et al., "Rapid detection of a highly virulent Chinese-type isolate of Porcine Reproductive and Respiratory Syndrome virus by real-time reverse transcriptase PCR, " Journal of Virological Methods, vol. 149, no. 1, pp. 49-55, 2008.
13. S. Xiao, Q. Wang, J. Jia, et al., "Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by twodimensional fluorescence difference gel electrophoresis, " Virology Journal, vol. 7, article 107, 2010.
14. Z.-Z. Yang, W.-H. Fang, and M. Habib, "First results of detection of PRRSV and CSFV RNA by SYBR green I-based quantitative PCR, " Journal of Veterinary Medicine B: Infectious Diseases and Veterinary Public Health, vol. 53, no. 10, pp. 461-467, 2006.
15. H. Tian, J. Wu, Y. Shang, Y. Chen, and X. Liu, "The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus, " Virology Journal, vol. 7, article 90, 2010.
16. S. B. Kleiboeker, S. K. Schommer, S.-M. Lee, S. Watkins, W. Chittick, and D. Polson, "Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative reverse transcriptase-PCR, " Journal of Veterinary Diagnostic Investigation, vol. 17, no. 2, pp. 165-170, 2005.