RT-qPCR-based quantification of small non-coding RNAs

Methods in Molecular Biology

Volumn 1296, Issue , 2015, Pages 85-102

  Zeka, Fjoralba ^a   Mestdagh, Pieter ^a   Vandesompele, Jo ^a  

^a NONE

Author keywords

MicroRNA; miScript; Preamplification; qPCR; Quality control; Reverse transcription reaction

Indexed keywords

MICRORNA; RIBONUCLEASE; SMALL UNTRANSLATED RNA; OLIGONUCLEOTIDE;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; PRIORITY JOURNAL; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA ISOLATION; RNA SEQUENCE; WORKFLOW; GENETICS; HUMAN; PROCEDURES;

HUMANS; MICRORNAS; OLIGONUCLEOTIDES; QUALITY CONTROL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84925339241     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2547-6_9     Document Type: Article

References (13)

1. Krol J, Loedige I, Filipowicz W (2010) The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Genet 11:597-610
2. Kozomara A, Griffiths-Jones S (2013) miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res 42:D68-D73
3. Berezikov E (2011) Evolution of microRNA diversity and regulation in animals. Nat Rev Genet 12:846-860
4. Soifer HS, Rossi JJ, Sætrom P (2007) MicroRNAs in disease and potential therapeutic applications. Mol Ther 15:2070-2079
5. Lesko LJ (2007) Personalized medicine: elusive dream or imminent reality? Clin Pharmacol Ther 81:807-816
6. Schwarzenbach H, Hoon DSB, Pantel K (2011) Cell-free nucleic acids as biomarkers in cancer patients. Nat Cell Biol 11:426-437
7. Mestdagh P, Hartmann N, Baeriswyl L, Andreasen D, Bernard N, Chen C, Cheo D, D'Andrade P, DeMayo M, Dennis L, Derveaux S, Feng Y, Fulmer SS, Gerstmayer B, Gouffon J, Grimley C, Lader E, Lee KY, Luo S, Mouritzen P, Narayanan A, Patel S, Peiffer S, Rüberg S, Schroth G, Schuster D, Shaffer JM, Shelton EJ, Silveria S, Ulmanella U, Veeramachaneni V, Staedtler F, Peters T, Guettouche T, Wong L, Vandesompele J (2014) Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study. Nat Methods 11:809-815
8. Mestdagh P, Van Vlierberghe P, De Weer A, Muth D, Westermann F, Speleman F, Vandesompele J (2009) A novel and universal method for microRNA RT-qPCR data normalization. Genome Biol 10:R64
9. D'haene B, Mestdagh P, Hellemans J, Vandesompele J (2012) MiRNA expression profiling: from reference genes to global mean normalization. Methods Mol Biol 822: 261-272
10. Ståhlberg A, Håkansson J, Xian X, Semb H, Kubista M (2004) Properties of the reverse transcription reaction in mRNA quantification. Clin Chem 50:509-515
11. Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8:R19
12. Aranda R, Dineen SM, Craig RL, Guerrieri RA, Robertson JM (2009) Comparison and evaluation of RNA quantification methods. Anal Biochem 387:122-127
13. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3 research0034.1-1333